|本期目录/Table of Contents|

[1]陈辉辉,金磊磊,喻镇东,等.1株侵染互花米草海洋真菌Buergenerula spartinae YDC07的分离鉴定[J].江苏农业科学,2015,43(01):342-346.
 Chen Huihui,et al.Isolation and identification of marine fungi Buergenerula spartinae YDC07 infecting Spartina alterniflora[J].Jiangsu Agricultural Sciences,2015,43(01):342-346.
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1株侵染互花米草海洋真菌Buergenerula spartinae YDC07
的分离鉴定
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第43卷
期数:
2015年01期
页码:
342-346
栏目:
资源与环境
出版日期:
2015-01-25

文章信息/Info

Title:
Isolation and identification of marine fungi Buergenerula spartinae YDC07 infecting Spartina alterniflora
作者:
陈辉辉12 金磊磊1 喻镇东12 姜晓龙12 绍荣2 许伟2 陈集双1
1.南京工业大学生物资源工程研究所,江苏南京 210009; 2.盐城工学院,江苏盐城 224005
Author(s):
Chen Huihuiet al
关键词:
互花米草海洋真菌Buergenerula spartinaerRNA-ITS进化分析
Keywords:
-
分类号:
Q949.32
DOI:
-
文献标志码:
A
摘要:
对侵染中国江苏沿海滩涂盐碱地的互花米草茎和叶上真菌进行分离纯化,得到1株与侵染互花米草海洋真菌形态极其相似的菌株YDC07。对菌株YDC07进行培养性状观察和形态学鉴定,发现该菌与中国新记录菌属互花米草比尔格纳菌十分相似;通过rRNA基因ITS序列分子生物学鉴定,发现该菌与比尔格纳菌属中互花米草比尔格纳菌相似度为96%~99%,在进化树中显示亲缘关系最近;该真菌可以侵染互花米草秸秆,使其生物量逐渐减少。菌株YDC07 rRNA-ITS序列相关信息提交NCBI数据库,获得GenBank登录号为KJ459363。菌株YDC07是在江苏沿海滩涂盐碱地互花米草上新发现的一种比尔格纳菌属海洋真菌。
Abstract:
-

参考文献/References:

[1]Wang G,Qin P,Wan S W,et al. Ecological control and integral utilization of Spartina alterniflora[J]. Ecological Engineering,2008,32(3):249-255.
[2]Simenstad C,Thom R. Spartina alterniflora(smooth cordgrass)as an invasive halophyte in Pacific northwest estuaries[J]. Hortus Northwest,1995,6(12):38-40.
[3]李富荣,陈俊勤,陈沐荣,等. 互花米草防治研究进展[J]. 生态环境,2007,16(6):1795-1800.
[4]Kohlmeyer J,Volkmann-Kohlmeyer B. Fungi on Juncus and Spartina:new marine species of Anthostomella,with a list of marine fungi known on Spartina[J]. Mycological Research,2002,106(3):365-374.
[5]Gessner R V. Seasonal occurrence and distribution of fungi associated with Spartina alterniflora from a Rhode Island estuary[J]. Mycologia,1977,69(3):477-491.
[6]Bergbauer M,Newell S Y. Contribution to lignocellulose degradation and DOC formation from a salt marsh macrophyte by the ascomycete Phaeosphaeria spartinicola[J]. FEMS Microbiology Ecology,1992,9(4):341-347.
[7]Newell S Y,Porter D. Microbial secondary production from salt marsh-grass shoots,and its known and potential fates[J]. Kluwer Academic,2000,72:159-185.
[8]Newell S Y,Porter D,Lingle W L. Lignocellulolysis by ascomycetes (fungi) of a saltmarsh grass (smooth cordgrass)[J]. Microscopy Res Techn,1996,33:32-46.
[9]匡治州,许杨. 核糖体rDNA ITS序列在真菌学研究中的应用[J]. 生命的化学,2004,24(2):120-122.
[10]Aanen D K,Kuyper T W,Hoekstra R F. A widely distributed ITS polymorphism within a biological species of the ectomycorrhizal fungus Hebeloma velutipes[J]. Mycological Research,2001,105(3):284-290.
[11]Hietala A M,Vahala J,Hantula J. Molecular evidence suggests that Ceratobasidium bicorne has an anamorph known as a conifer pathogen[J]. Mycological Research,2001,105(5):555-562.
[12]Okabe I,Arakawa M,Matsumoto N. ITS polymorphism within a single strain of Sclerotium rolfsii[J]. Mycoscience,2001,42(1):107-113.
[13]方中达. 植病研究方法[M]. 北京:中国农业出版社,1998.
[14]刘崇玉. 植物内生真菌HCCB05974和HCCB06030次级代谢产物的分离纯化[D]. 上海:上海交通大学,2012.
[15]杨宴霞. 放线菌SPRI-710055次级代谢产物中除草活性成分研究及菌种鉴定[D]. 上海:上海师范大学,2009.
[16]刘谦. 海洋放线菌TXC6-6次级代谢产物中活性组分的分离纯化、结构和菌种的鉴定[D]. 上海:上海师范大学,2012.
[17]刘岩岩,张鹭. 一株乳酸菌培养条件优化及菌种鉴定[J]. 齐齐哈尔大学学报,2008,24(2):21-24.
[18]Sambrook J,Russell D W. Molecular cloning:a laboratory manual[M]. New York:Cold Spring Harbor Laboratory Press,2001.
[19]刘春来,文景芝,杨明秀,等. rDNA-ITS在植物病原真菌分子检测中的应用[J]. 东北农业大学学报,2007,38(1):101-106.
[20]Thompson J D,Higgins D G,Gibson T J. CLUSTAL W:improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice[J]. Nucleic Acids Research,1994,22(22):4673-4680.
[21]Tamura K,Dudley J,Nei M,et al. MEGA4:molecular evolutionary genetics analysis(MEGA) software version 4.0[J]. Molecular Biology and Evolution,2007,24(8):1596-1599.
[22]Walker J. Taxonomy of take-all fungi and related genera and species[J]. Biology and Control of Take-all,1981,29:15-74.
[23]赵杰. ITS序列分析及其在植物真菌病害分子检测中的应用[J]. 陕西农业科学,2004(4):35-37.
[24]Newell S Y. Spore-expulsion rates and extents of blade occupation by ascomycetes of the smooth-cordgrass standing-decay system[J]. Botanica Marina,2001,44(3):277-285.
[25]王春兰,朱品,黄睿,等. 杭州地区一株侵染水稻的嗜水小核菌菌株的鉴定[J]. 科技通报,2013,29(9):55-60.
[26]Schroeder S,Kim S H,Cheung W T,et al. Phylogenetic relationship of ophiostoma piliferum to other sapstain fungi based on the nuclear rRNA gene[J]. FEMS Microbiology Letters,2001,195(2):163-167.
[27]O′donnell K. Ribosomal DNA internal transcribed spacers are highly divergent in the phytopathogenic ascomycete Fusarium sambucinum(Gibberella pulicaris)[J]. Current Genetics,1992,22(3):213-220.
[28]de Los Angeles Vineusa M,Sanches-Puelles J M,Tibell L. Intraspecific variation in Mycocalicium subtile(Mycocaliciaceae) elucidated by morphology and the sequences of the ITS1-5.8S-ITS2 region of rDNA[J]. Mycological Research,2001,105(3):323-330.
[29]Yan Z H,Rogers S O,Wang C J K. Assessment of Phialophora species based on ribosomal DNA internal transcribed spacers and morphology[J]. Mycologia,1995,87(1):72-83.

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备注/Memo

备注/Memo:
收稿日期:2014-03-31
基金项目:国家自然科学基金(编号:30971898)。
作者简介:陈辉辉(1988—),男,江苏南京人,硕士,主要从事植物真菌及病毒研究。E-mail:huihui_chen2012@163.com。
通信作者:陈集双,博士,教授,主要从事植物真菌、病毒、植物反应器、秸秆资源化和产业化利用等研究。E-mail:biochenjs@njut.edu.cn。
更新日期/Last Update: 2015-01-25