|本期目录/Table of Contents|

[1]李晓君,周文吉,周启武,等.ShSAP1的原核表达及多克隆抗体的制备[J].江苏农业科学,2015,43(03):43-45.
　Li Xiaojun,et al.Prokaryotic expression of ShSAP1 and preparation of its polyclonal antibodies[J].Jiangsu Agricultural Sciences,2015,43(03):43-45.
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ShSAP1的原核表达及多克隆抗体的制备(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第43卷
期数:
2015年03期
页码:
43-45
栏目:
生物技术
出版日期:
2015-03-25

文章信息/Info

Title:
Prokaryotic expression of ShSAP1 and preparation of its polyclonal antibodies
作者:
李晓君1;  周文吉1;  周启武1;  王文荣2
1.临沧师范高等专科学校农学系，云南临沧 677000； 2.云南省临沧市甘蔗技术推广站，云南临沧 677000
Author(s):
Li Xiaojun; et al
关键词:
甘蔗; ShSAP1; 锌指蛋白; 原核表达; 多克隆抗体
Keywords:
-
分类号:
Q331
DOI:
-
文献标志码:
A
摘要:
将ShSAP1的阅读框连接到原核表达载体pET32a(+)中，构建成ShSAP1原核表达载体PET-ShSAP1，然后将其转入宿主菌BL21，经IPTG诱导后，宿主菌表达出与预期分子量大小相符的35 ku的融合蛋白；将纯化后的融合蛋白免疫家兔，获得了ShSAP1的特异性抗血清；以融合蛋白作抗原，用间接ELISA法测定其抗血清效价为1 ∶25 000。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2014-05-06
基金项目：现代农业产业技术体系(甘蔗)建设专项资金(编号：CARs-20-6-14)；临沧师范高等专科学校校级课题(编号：LCSZL2013001)。
作者简介：李晓君(1985—)，女，云南临沧人，博士，讲师，主要从事植物生物技术与种质创新方面研究。E-mail：lxj-148@163.com。
通信作者：王文荣。E-mail：lcgks@163.com。
更新日期/Last Update: 2015-03-25