[1]宗桦,胡霞,陈学宏.侧枝萌发诱导基因(LIF)的原核表达与农杆菌介导转化麻风树幼叶[J].江苏农业科学,2015,43(09):14-19.
　Zong Hua,et al.Prokaryotic expression and agrobacterium-mediated transformation of Jatropha curcas young leaf explants with lateral shoot-inducing factor (LIF)[J].Jiangsu Agricultural Sciences,2015,43(09):14-19.
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侧枝萌发诱导基因(LIF)的原核表达
与农杆菌介导转化麻风树幼叶()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第43卷
期数:
2015年09期
页码:
14-19
栏目:
生物技术
出版日期:
2015-09-25

文章信息/Info

Title:
Prokaryotic expression and agrobacterium-mediated transformation of Jatropha curcas young leaf explants with lateral shoot-inducing factor (LIF)
作者:
宗桦1;  胡霞2;  陈学宏3
1.西南交通大学建筑学院，四川成都 610031；2.乐山师范学院生命科学学院，四川乐山 614004；
3.西南交通大学生命科学与工程学院，四川成都 610031
Author(s):
Zong Hua; et al
关键词:
麻风树; 侧枝萌发诱导基因(LIF); 幼叶; 农杆菌
Keywords:
-
分类号:
Q943.2
DOI:
-
文献标志码:
A
摘要:
麻风树(Jatropha curcas L.)的有限分枝是限制麻风树油产量的最主要因素之一，为了奠定基因调控麻风树分枝的基础，研究并优化了侧枝萌发诱导基因LIF(lateral shoot-inducing factor )的原核表达条件，并成功获得了重组蛋白。通过研究农杆菌浓度、抗生素浓度建立了稳固高效的麻风树农杆菌((LBA4404)高效转化体系；将LIF基因转入麻风树基因组中，通过GUS染色、PCR、Southern blot检测，确定了LIF基因已经成功转化入麻风树基因组中，获得了高达(23.91±5.78)%的转化率。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2014-08-28
基金项目：国家自然科学基金(编号：51308464)；西南交通大学科技创新项目(编号：A0920502051405-62)。
作者简介：宗桦(1981—)，女，江苏宜兴人，博士，讲师，主要从事植物学研究。E-mail：14744632@qq.com。
更新日期/Last Update: 2015-09-25