|本期目录/Table of Contents|

[1]郜卫华,田罗,黄廷华,等.凡纳滨对虾儿茶酚-氧位-甲基转移酶cDNA序列克隆及组织表达分析[J].江苏农业科学,2016,44(02):26-30.
　Gao Weihua,et al.Cloning，characterization and expression of full length cDNA encoding catechol-O-methyltransferase in Litopenaeus vannamei[J].Jiangsu Agricultural Sciences,2016,44(02):26-30.
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凡纳滨对虾儿茶酚-氧位-甲基转移酶
cDNA序列克隆及组织表达分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第44卷
期数:
2016年02期
页码:
26-30
栏目:
生物技术
出版日期:
2016-02-25

文章信息/Info

Title:
Cloning，characterization and expression of full length cDNA encoding catechol-O-methyltransferase in Litopenaeus vannamei
作者:
郜卫华1; 2;  田罗3;  黄廷华1;  姚敏1;  许巧情1; 2
1.长江大学动物科学学院水产系，湖北荆州 434025；2.汕头大学广东省海洋生物技术重点实验室，广东汕头 515063；
3. 荆州职业技术学院，湖北荆州 434023
Author(s):
Gao Weihua; et al
关键词:
凡纳滨对虾; 儿茶酚-氧位-甲基转移酶; 基因克隆; 表达
Keywords:
-
分类号:
S917；Q786
DOI:
-
文献标志码:
A
摘要:
利用RACE (rapid amplification of cDNA ends)技术，克隆了凡纳滨对虾儿茶酚-氧位-甲基转移酶(catechol-O-methyltransferase，COMT)基因cDNA序列。该基因(LvCOMT)cDNA全长910 bp(GenBank登录号JQ345478)，含有666 bp的开放阅读框(ORF)，编码221个氨基酸，理论分子量74.9 ku，等电点4.98。经软件分析，该成熟肽有12个磷酸化的氨基酸位点，不具信号肽，推测凡纳滨对虾COMT基因可能是依赖12个可能的氨基酸发生可逆的磷酸化反应来进行调控作用。LvCOMT基因与斑节对虾、中国明对虾的氧位-甲基转移酶具有高度相似性，系统进化分析表明，LvCOMT在亲缘关系上更接近于哺乳动物的儿茶酚-氧位-甲基转移酶。应用RQ-PCR分析COMT在凡纳滨对虾中的组织特异性分布，结果显示COMT在肝胰腺组织中表达量最高，在鳃中的表达量最低。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2015-03-06
基金项目：长江大学博士启动经费(编号：8012000101122)；广东省海洋生物技术重点实验室开放基金(编号：GPKLMB201403)。
作者简介：郜卫华(1977—)，女，湖北襄阳人，博士，讲师，主要从事水产分子营养学研究。E-mail：gwh@126.com。
通信作者：许巧情，博士，教授，主要从事水产分子生物学研究。E-mail：35507883@qq.com。
更新日期/Last Update: 2016-02-25