|本期目录/Table of Contents|

[1]麻锐,冯凯,姜锐,等.不同生长年限园参根的双向电泳分析[J].江苏农业科学,2016,44(10):84-88.
　Ma Rui,et al.Study on ginseng roots with different growth years by two-dimensional electrophoresis[J].Jiangsu Agricultural Sciences,2016,44(10):84-88.
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不同生长年限园参根的双向电泳分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第44卷
期数:
2016年10期
页码:
84-88
栏目:
生物技术
出版日期:
2016-10-25

文章信息/Info

Title:
Study on ginseng roots with different growth years by two-dimensional electrophoresis
作者:
麻锐;  冯凯;  姜锐;  陈伟;  靳雯棋;  徐晓浩;  毕英飞;  孙立伟
北华大学化学与生物学院，吉林吉林 132013
Author(s):
Ma Rui; et al
关键词:
园参; 生长年限; 双向电泳; 蛋白质组学
Keywords:
-
分类号:
S567.5+10.1
DOI:
-
文献标志码:
A
摘要:
为了解园参根在生长过程中的蛋白质组学变化，利用双向电泳技术分离1~5年园参根蛋白，重点分析园参根生长(3年)、成熟(5年)之间的蛋白质组学差异，并利用MALDI-TOF/TOF鉴定差异显著的81个蛋白点。结果表明：有19个差异蛋白点在成熟期表达量下调，62个蛋白点表达量上调，针对其与园参根生长发育的潜在关系进行分类与讨论；园参根在逐渐成熟的过程中与抗胁迫相关的过氧化氢酶、单脱氢抗坏血酸还原酶等，以及与能量代谢相关的ATP酶、甘油醛-3-磷酸脱氢酶等表达量增加，而与生长相关的果胶酯酶表达量减少，这些蛋白质的变化对于探讨人参根的生长发育规律有重要意义。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2015-09-02
基金项目：国家自然科学基金(编号：81373932)；吉林省教育厅科学技术研究项目［编号：吉教科合字(2014)第193号］；吉林省科技计划(编号：20150520138JH、20130522045JH、20140519016JH、YYZX201268)。
作者简介：麻锐(1982—)，女，吉林吉林人，博士，讲师，主要从事人参蛋白质组学研究。E-mail：maruijilin@163.com。
通信作者：冯凯，硕士，讲师，主要从事植物生物科学研究。E-mail：fengk544@163.com。
更新日期/Last Update: 2016-10-25