|本期目录/Table of Contents|

[1]郎刚华,顾立众,李西腾.大米α-球蛋白的原核表达、抗体制备及检测[J].江苏农业科学,2016,44(11):34-37,113.
　Lang Ganghua,et al.Prokaryotic expression，antibody preparation and detection of α-globulin in rice[J].Jiangsu Agricultural Sciences,2016,44(11):34-37,113.
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大米α-球蛋白的原核表达、抗体制备及检测(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第44卷
期数:
2016年11期
页码:
34-37,113
栏目:
生物技术
出版日期:
2016-11-25

文章信息/Info

Title:
Prokaryotic expression，antibody preparation and detection of α-globulin in rice
作者:
郎刚华;  顾立众;  李西腾
江苏食品药品职业技术学院，江苏淮安 223003
Author(s):
Lang Ganghua; et al
关键词:
大米; α-球蛋白; 原核表达; 抗体; 检测分析; Western Blotting; ELISA
Keywords:
-
分类号:
TS201.2+1
DOI:
-
文献标志码:
A
摘要:
大米α-球蛋白是大米胚乳中主要的盐溶蛋白，也是大米的主要致敏原之一。为了建立起大米α-球蛋白的检测分析方法，将大米α-球蛋白的编码基因亚克隆到表达载体pQE30中，并在大肠杆菌中进行了重组大米α-球蛋白的表达。通过金属亲和层析法对重组大米α-球蛋白进行纯化，以纯化的重组大米α-球蛋白为抗原免疫白兔，制备出了抗大米α-球蛋白多克隆抗体。Western Blotting 检测结果显示，该抗体能够特异性地与大米α-球蛋白进行免疫结合反应，抗大米α-球蛋白抗体与目标蛋白的结合呈现出一定的定量关系。并且，利用该抗体构建起了大米α-球蛋白的 ELISA检测分析方法，并对3种大米样品中α-球蛋白的含量进行了检测分析。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2015-10-11
基金项目：江苏省自然科学基金面上项目(编号：BK20151286)。
作者简介：郎刚华(1969—)，山东青岛人，博士，讲师，主要从事食品致敏原及转基因食品安全性研究。E-mail：dichen123451@foxmail.com。
更新日期/Last Update: 2016-11-25