[1]薛涛涛,边金,伍陆,等.酿酒酵母组蛋白H3K56A突变菌株的构建及其对细胞生长的影响[J].江苏农业科学,2016,44(12):77-81.
　Xue Taotao,et al.Construction of histone H3K56A mutant of Saccharomyces cerevisiae and its effects on cell growth[J].Jiangsu Agricultural Sciences,2016,44(12):77-81.
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酿酒酵母组蛋白H3K56A突变菌株的构建
及其对细胞生长的影响()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第44卷
期数:
2016年12期
页码:
77-81
栏目:
生物技术
出版日期:
2016-12-25

文章信息/Info

Title:
Construction of histone H3K56A mutant of Saccharomyces cerevisiae and its effects on cell growth
作者:
薛涛涛; 边金; 伍陆; 赵秀娟; 崔向军; 蔡禄
内蒙古科技大学数理与生物工程学院，内蒙古包头 014010
Author(s):
Xue Taotao; et al
关键词:
酿酒酵母; H3K56A突变菌; 组蛋白修饰; 两步替换法; 同源重组; 高盐环境; 高温环境; 抗逆机制
Keywords:
-
分类号:
S188
DOI:
-
文献标志码:
A
摘要:
组蛋白修饰，例如甲基化、乙酰化等修饰对基因表达和细胞生长至关重要。为揭示组蛋白H3第56位赖氨酸(K)修饰对酵母细胞生长的重要性，构建H3K56定点突变为丙氨酸(A)的组蛋白突变株H3K56A，并对其在高温、高盐等条件下的生长状况进行初步检测。根据酵母同源重组的原理，采用两步替换法构建H3K56A突变菌株，用分光光度法测定突变菌株的生长曲线，并分别在高盐、高温条件下检测突变体的表型。结果表明，酿酒酵母组蛋白H3K56A突变菌株构建成功，其生长曲线与野生型无明显差异；H3K56A突变株对高温、高盐条件均较敏感，且在高盐条件下，菌体生长缓慢、菌落偏小，表明组蛋白H3K56位点的修饰可能与酿酒酵母抗高盐、高温机制有关。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2015-11-02
基金项目：国家自然科学基金(编号：61361014、31260274)；内蒙古自然科学基金(编号：2015MS0334)。
作者简介：薛涛涛(1990—)，女，山西临县人，硕士研究生，主要从事表观遗传学研究。E-mail：xuetaotao196485@163.com。
通信作者：赵秀娟，博士，教授，主要从事表观遗传学研究。E-mail：zhaoxiujuan@imust.cn。
更新日期/Last Update: 2016-12-25