|本期目录/Table of Contents|

[1]王晓醒,李淑娟,谢永丽,等.梭罗草AFLP体系优化及指纹图谱构建[J].江苏农业科学,2017,45(16):37-41.
 Wang Xiaoxing,et al.Optimization of AFLP system and construction of fingerprint of Kengyilia thoroldiana[J].Jiangsu Agricultural Sciences,2017,45(16):37-41.
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梭罗草AFLP体系优化及指纹图谱构建(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第45卷
期数:
2017年16期
页码:
37-41
栏目:
生物技术
出版日期:
2017-08-20

文章信息/Info

Title:
Optimization of AFLP system and construction of fingerprint of Kengyilia thoroldiana
作者:
王晓醒 李淑娟 谢永丽 李长慧
青海大学农牧学院,青海西宁 810003
Author(s):
Wang Xiaoxinget al
关键词:
梭罗草指纹图谱AFLP体系以礼草属预扩增选扩增引物种质鉴定
Keywords:
-
分类号:
Q755
DOI:
-
文献标志码:
A
摘要:
利用梭罗草(Kengyilia thoroldiana)基因组DNA优化并建立的梭罗草扩增片段长度多态性(amplified fragment length polymorphism,简称AFLP)反应体系。预扩增反应体系25 μL:连接产物1 μL,10×PCR Buffer 2.5 μL,dNTP 0.375 μL,Mg2+ 1 μL,Taq DNA聚合酶0.5 μL,EcoRⅠ预扩引物1 μL,MseⅠ预扩引物1.2 μL,ddH2O加至 25 μL;选扩增反应体系25 μL:预扩产物(稀释20倍)2.0 μL,10×PCR Buffer 2.5 μL,dNTP 0.60 μL,Mg2+2 μL,TaqDNA聚合酶0.1 μL,EcoR Ⅰ选扩引物1.5 μL,Mse I选扩引物2.5 μL,ddH2O加至25 μL。利用优化后的AFLP体系,从梭罗草、大颖草(K. grandiglumis)、疏花以礼草(K. laxiflora)共10份供试材料中筛选出216对选择性扩增引物,从中筛选出38对具有多态性的引物并从中选出6对条带清晰、鉴别效率高的引物,其中E16C4、E4C10的扩增条带中均有梭罗草的特征条带,为梭罗草的种质鉴定及利用提供科学依据。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期:2016-04-14
基金项目:国家自然科学基金(编号:31360578)。
作者简介:王晓醒(1991—),女,河北石家庄人,硕士研究生,研究方向为牧草饲料开发利用。E-mail:694840579@qq.com。
通信作者:李长慧,博士,教授,研究方向为牧草饲料开发利用。E-mail:746886595@qq.com。
更新日期/Last Update: 2017-08-20