|本期目录/Table of Contents|

[1]陈晓燕,黄明珠,张妮,等.南美白对虾CuZnSOD原核表达及抗体制备[J].江苏农业科学,2017,45(20):193-195.
 Chen Xiaoyan,et al.Prokaryotic expression and polyclonal antibody of extracellular copper-zinc superoxide dismutase from white shrimp,Litopenaeus vannamei[J].Jiangsu Agricultural Sciences,2017,45(20):193-195.
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南美白对虾CuZnSOD原核表达及抗体制备(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第45卷
期数:
2017年20期
页码:
193-195
栏目:
畜牧兽医与水产蚕桑
出版日期:
2017-10-20

文章信息/Info

Title:
Prokaryotic expression and polyclonal antibody of extracellular copper-zinc superoxide dismutase from white shrimp,Litopenaeus vannamei
作者:
陈晓燕 黄明珠 张妮 刘欢 朱芳芳 熊志伟
江西科技学院护理学院,江西南昌 330098
Author(s):
Chen Xiaoyanet al
关键词:
胞外CuZnSOD原核表达纯化组织分布
Keywords:
-
分类号:
S917
DOI:
-
文献标志码:
A
摘要:
锌铜超氧化物歧化酶(cytoplasmic copper/zinc superoxide dismutase,CuZnSOD)是超氧化物歧化酶家族的一种,超氧化物歧化酶在动植物的抗氧化和免疫功能中扮演着重要的角色。本研究将南美白对虾胞外CuZnSOD基因与pet32a表达载体连接,转化大肠杆菌BL21,IPTG诱导融合表达,经SDS-PAGE分析表明,融合蛋白主要以包涵体形式存在,分子量约为18 ku。利用Ni柱亲和纯化发酵产物,得到较单一的蛋白,制备了多克隆抗体。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2016-05-19
基金项目:江西省教育厅科技研究项目(编号:GJJ151148);江西科技学院青年基金项目(编号:ZR15QN06)。
作者简介:陈晓燕(1984—),女,江西抚州人,硕士,讲师,主要从事药学研究。
通信作者:黄明珠,硕士研究生,主要从事动物分子免疫学研究。E-mail:491951061@qq.com。
更新日期/Last Update: 2017-10-20