|本期目录/Table of Contents|

[1]金伟,陈文静,缪礼鸿,等.1株耐热纤维素酶产生菌的筛选及其酶学特性[J].江苏农业科学,2017,45(20):272-274.
 Jin Wei,et al.Isolation of a thermotolent cellulase-producing aspergillus fumigatus strain and its cellulase characterization[J].Jiangsu Agricultural Sciences,2017,45(20):272-274.
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第45卷
期数:
2017年20期
页码:
272-274
栏目:
资源与环境
出版日期:
2017-10-20

文章信息/Info

Title:
Isolation of a thermotolent cellulase-producing aspergillus fumigatus strain and its cellulase characterization
作者:
金伟 陈文静 缪礼鸿 刘蒲临
武汉轻工大学生物与制药工程学院,湖北武汉 430023
Author(s):
Jin Weiet al
关键词:
纤维素酶烟曲霉耐高温真菌筛选木薯酒糟培养液
Keywords:
-
分类号:
Q939.96;S182
DOI:
-
文献标志码:
A
摘要:
采用富集培养并结合滤纸条降解法进行初筛,从土壤中分离到1株产纤维素酶能力较强的耐热真菌XT3。通过菌株的形态特征和18S rDNA序列同源性比较,该菌株被鉴定为烟曲霉(Aspergillus fumigatus)。发酵试验表明,XT3菌株产酶培养的最适起始pH值为4.0,最适产酶发酵温度为45 ℃;该菌株产生的羧甲基纤维素(CMC)酶和滤纸酶的最适作用温度分别为80、70 ℃,具有较好的耐热性、耐酸性。此外,研究还表明,XT3菌株能够较好地利用木薯酒糟液发酵产纤维素酶,在木薯酒糟液培养基中添加2%麸皮可使发酵液的纤维素酶活性提高2.1倍,表明该菌株具有潜在的应用价值。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2016-04-18
基金项目:国家“863”计划(编号:2013AA102805-04)。
作者简介:金伟(1986—),男,湖北武汉人,硕士,主要从事资源与环境微生物学研究。E-mail:93332945@qq.com。
通信作者:缪礼鸿,博士,教授,主要从事资源与环境微生物学研究。E-mail:miaowhpu@126.com。
更新日期/Last Update: 2017-10-20