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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第46卷

期数:

2018年08期

页码:

192-194

栏目:

贮藏加工与检测分析

出版日期:

2018-04-20

文章信息/Info

Title:

Purification and application of β-glucosidse from Agrocybe aegerita

作者:

陈杏;  杨成丽;  鲍炳鑫;  张璇; 蒲 姝瑾;  张煦彤;  李大力;  石若夫

南京理工大学生物工程系，江苏南京 210094

Author(s):

Chen Xing; et al

关键词:

茶树菇; β-葡萄糖苷酶; 纤维素酶; 喜树残渣; 糖化; 纯化

Keywords:

-

分类号:

R284.2

DOI:

-

文献标志码:

A

摘要:

从茶树菇中分离纯化一种β-葡萄糖苷酶，并对其进行酶学性质的研究。该β-葡萄糖苷酶的分子量为 26 ku，最适pH值为6.0，最适温度为50 ℃。乙醇浸提喜树碱后的喜树果渣经β-葡萄糖苷酶和纤维素酶协同水解后，还原糖和葡萄糖的生成量分别为0.90、0.69 mg/mL。研究表明，茶树菇β-葡萄糖苷酶可应用于中草药提取残渣的生物水解领域。

Abstract:

-

参考文献/References:

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备注/Memo

备注/Memo:

收稿日期：2016-11-29
作者简介：陈杏(1992—)，女，湖北石首人，硕士研究生，主要从事生物催化研究。E-mail：13770319302@139.com。
通信作者：杨成丽，博士，副教授，主要从事生物催化研究。 E-mail：bioedu@sina.com。

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更新日期/Last Update: 2018-04-20