|本期目录/Table of Contents|

[1]李霄,陈婷,鲍玲莉,等.甘蓝型油菜BnNPR1的序列及其环境信号响应分析[J].江苏农业科学,2018,46(14):19-24.
　Li Xiao,et al.Sequence analysis of BnNPR1 in Brassica napus and its responses to environmental signals[J].Jiangsu Agricultural Sciences,2018,46(14):19-24.
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甘蓝型油菜BnNPR1的序列及其环境信号响应分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第46卷
期数:
2018年第14期
页码:
19-24
栏目:
生物技术
出版日期:
2018-07-25

文章信息/Info

Title:
Sequence analysis of BnNPR1 in Brassica napus and its responses to environmental signals
作者:
李霄;  陈婷;  鲍玲莉;  谭小力;  张志燕;  王政
江苏大学生命科学研究院，江苏镇江 212013
Author(s):
Li Xiao; et al
关键词:
甘蓝型油菜; BnNPR1; 环境信号; 表达响应; 基因克隆; 序列分析
Keywords:
-
分类号:
S565.401
DOI:
-
文献标志码:
A
摘要:
为了研究油菜NPR1(nonexpressor of pathogenesis-related genes 1)基因对各种环境胁迫信号及植物防卫激素信号的表达响应，以甘蓝型油菜品种宁油12为材料，采用同源克隆法克隆了甘蓝型油菜(Brassica napus L.) NPR1基因BnNPR1的cDNA序列。序列分析表明，BnNPR1编码的蛋白序列由579个氨基酸残基组成，含有1个锚定蛋白重复序列结构和1个BTB/POZ结构域，与AtNPR1的序列相似性达到73%。烟草叶片瞬时表达分析显示，BnNPR1编码1个细胞核定位蛋白；实时荧光定量PCR分析显示，BnNPR1在根、茎和叶组织器官中特异性表达，其中在根中表达量极为显著，并且对4 ℃低温、PEG溶液以及水杨酸和甲基茉莉酸等的处理呈显著上调表达，而对K2Cr2O7和NaCl溶液处理则呈显著下调表达。这些结果表明，BnNPR1是低温、重金属、干旱、高盐以及水杨酸(SA)和茉莉酸(JA)等防卫信号分子的响应基因，本研究结果将为进一步分析BnNPR1在油菜抗逆中的功能及其机制提供线索。
Abstract:
-

参考文献/References:

［1］Cao H，Glazebrook J，Clarke J D，et al. The Arabidopsis NPR1 gene that controls systemic acquired resistance encodes a novel protein containing ankyrin repeats［J］. Cell，1997，88(1)：57-63.
［2］Spoel S H，Koornneef A，Claessens S M，et al. NPR1 modulates cross-talk between salicylate- and jasmonate-dependent defense pathways through a novel function in the cytosol［J］. Plant Cell，2003，15(3)：760-770.
［3］Cao H，Bowling S A，Gordon A S，et al. Characterization of an Arabidopsis mutant that is nonresponsive to inducers of systemic acquired resistance［J］. Plant Cell，1994，6(11)：1583-1592.
［4］Yu D，Chen C，Chen Z. Evidence for an important role of WRKY DNA binding proteins in the regulation of NPR1 gene expression［J］. Plant Cell，2001，13(7)：1527-1540.
［5］Ryals J，Weymann K，Lawton K，et al. The Arabidopsis NIM1 protein shows homology to the mammalian transcription factor inhibitor I kappa B［J］. Plant Cell，1997，9(3)：425-439.
［6］Mou Z，Fan W，Dong X. Inducers of plant systemic acquired resistance regulate NPR1 function through redox changes［J］. Cell，2003，113(7)：935-944.
［7］Sugano S，Jiang C J，Miyazawa S I，et al. Role of OsNPR1 in rice defense program as revealed by genome-wide expression analysis［J］. Plant Molecular Biology，2010，74(6)：549-562.
［8］Zhang J Y，Qu S C，Qiao Y S，et al. Overexpression of the Malus hupehensis MhNPR1 gene increased tolerance to salt and osmotic stress in transgenic tobacco［J］. Molecular Biology Reports，2014，41(3)：1553-1561.
［9］Jayakannan M，Bose J，Babourina O，et al. Salicylic acid in plant salinity stress signalling and tolerance［J］. Plant Growth Regulation，2015，76(1)：25-40.
［10］Jayakannan M，Bose J，Babourina O，et al. The NPR1-dependent salicylic acid signalling pathway is pivotal for enhanced salt and oxidative stress tolerance in Arabidopsis［J］. Journal of Experimental Botany，2015，66(7)：1865-1875.
［11］Rengasamy P. World salinization with emphasis on Australia［J］. Journal of Experimental Botany，2006，57(5)：1017-1023.
［12］Liu J Y，Zhu Y，Huang L L，et al. Combined effect of ethylene- and salicylic acid-signaling insensitive mutation on Arabidopsis response to low temperature［J］. Biologia Plantarum，2016，60(3)：523-531.
［13］Xia J C，Zhao H，Liu W Z，et al. Role of cytokinin and salicylic acid in plant growth at low temperatures［J］. Plant Growth Regulation，2008，57(3)：211-221.
［14］Hao L，Zhao Y，Jin D D，et al. Salicylic acid-altering Arabidopsis mutants response to salt stress［J］. Plant and Soil，2012，354(7)：81-95.
［15］Zhang J Y，Qu S C，Du X L，et al. Overexpression of the Malus hupehensis MhTGA2 gene，a novel bZIP transcription factor for increased tolerance to salt and osmotic stress in transgenic tobacco［J］. International Journal of Plant Sciences，2012，59(5)：441-453.
［16］Quilis J，Peas G，Messeguer J，et al. The Arabidopsis AtNPR1 inversely modulates defense responses against fungal，bacterial，or viral pathogens while conferring hypersensitivity to abiotic stresses in transgenic rice［J］. Molecular Plant-Microbe Interactions，2008，21(9)：1215-1231.
［17］陈宇. 油菜在多种逆境条件下qRT-PCR内参基因的稳定性评估及抗逆相关基因的表达分析［D］. 镇江：江苏大学，2014.
［18］Chou K C，Shen H B. Plant-mPLoc：a top-down strategy to augment the power for predicting plant protein subcellular localization［J］. PLoS One，2010，5(6)：e11335.
［19］Wang Z，Chen Y，Fang H，et al. Selection of reference genes for quantitative reverse-transcription polymerase chain reaction normalization in Brassica napus under various stress conditions［J］. Molecular Genetics and Genomics，2014，289(5)：1023-1035.
［20］汪雷，刘瑶，丁一娟，等. 油菜菌核病研究进展［J］. 西北农林科技大学学报(自然科学版)，2015，43(10)：85-93.
［21］Kallenbach M，Alagna F，Baldwin I T，et al. Nicotiana attenuata SIPK，WIPK，NPR1，and fatty acid-amino acid conjugates participate in the induction of jasmonic acid biosynthesis by affecting early enzymatic steps in the pathway［J］. Plant Signaling & Behavior，2010，152(1)：96-106.
［22］Yi S Y，Min S R，Kwon S Y. NPR1 is instrumental in priming for the enhanced flg22-induced MPK3 and MPK6 activation［J］. Plant Pathology Journal，2015，31(2)：192-194.

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备注/Memo

备注/Memo:
收稿日期：2017-01-22
基金项目：国家自然科学基金(编号：31071672)；江苏大学高级专业人才科研启动基金(编号：09JDG061)。
作者简介：李霄(1991—)，女，山东潍坊人，硕士，主要从事油菜功能基因组学方面的研究。E-mail：18252585951@163.com。
通信作者：王政，博士，副研究员，主要从事油菜功能基因组学方面的研究。E-mail：wangzheng4466@163.com。
更新日期/Last Update: 2018-07-20