|本期目录/Table of Contents|

[1]聂金梅,李阳源,刘金山,等.黑曲霉葡萄糖氧化酶基因改造及其在毕赤酵母中的表达[J].江苏农业科学,2018,46(20):17-21.
　Nie Jinmei,et al.Expression of modified GOD gene from Aspergillus niger in Pichia pastoris[J].Jiangsu Agricultural Sciences,2018,46(20):17-21.
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第46卷
期数:
2018年第20期
页码:
17-21
栏目:
生物技术
出版日期:
2018-10-20

文章信息/Info

Title:
Expression of modified GOD gene from Aspergillus niger in Pichia pastoris
作者:
聂金梅;  李阳源;  刘金山;  王勇;  唐业
广东溢多利生物科技股份有限公司，广东珠海 519060
Author(s):
Nie Jinmei; et al
关键词:
葡萄糖氧化酶; 黑曲霉; 毕赤酵母表达系统; 信号肽
Keywords:
-
分类号:
Q789
DOI:
-
文献标志码:
A
摘要:
为构建并筛选高效表达的葡萄糖氧化酶基因毕赤酵母工程菌株，采用PCR法从黑曲霉基因组中获得葡萄糖氧化酶全长基因(GOD)，采用定点突变法和重叠PCR法获得GODmut基因，将其插入到pPICZαA中，获得重组质粒pPIC-GODmut，经PmeI线性化后，用电击法转化毕赤酵母菌株X33，经大量筛选，获得1株高效分泌表达的工程菌株X33-pPIC-GODmut，在此基础上，去除GOD基因的信号肽，用同样的试验方法构建并筛选高效分泌表达的工程菌株X33-pPIC-GODnew，结果表明：X33-pPIC-GODmut的产酶水平为460.3 U/mL，X33-pPIC-GODnew的产酶水平为714.9 U/mL，是前者的1.52倍，该酶的最适作用温度和最适作用pH值分别为40 ℃和5.0，成功获得1株高效稳定表达的葡萄糖氧化酶基因毕赤酵母工程菌株。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2017-04-27
作者简介：聂金梅(1986—)，女，江西上饶人，硕士，工程师，主要从事分子生物学方面的研究。E-mail：njm1030@163.com。
更新日期/Last Update: 2018-10-20