|本期目录/Table of Contents|

[1]刘爽.2个不同番茄品系fasciated基因克隆及表达载体构建[J].江苏农业科学,2018,46(20):21-24.
 Liu Shuang,et al.Cloning and vector construction of fascinated Gene from two different tomato cultivars[J].Jiangsu Agricultural Sciences,2018,46(20):21-24.
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2个不同番茄品系fasciated基因克隆及表达载体构建(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第46卷
期数:
2018年第20期
页码:
21-24
栏目:
生物技术
出版日期:
2018-10-20

文章信息/Info

Title:
Cloning and vector construction of fascinated Gene from two different tomato cultivars
作者:
刘爽
福建农林大学园艺学院,福建福州 350002
Author(s):
Liu Shuanget al
关键词:
番茄心室fasciated载体构建Gateway
Keywords:
-
分类号:
S641.201
DOI:
-
文献标志码:
A
摘要:
为了比较由笔者所在实验室通过多代自交筛选得到的性状稳定的2个番茄品系FL1、MLK1中fasciated基因的编码序列,并验证该基因的功能,通过特异性引物PCR,获得该基因在2个供试材料中的序列,并通过Gateway技术分别构建了该基因的超量表达载体和RNAi载体。序列比对结果表明,2个心室差异明显的番茄材料中该基因的编码序列完全一致,同时也构建了完整的质粒表达载体系统。供试番茄材料中心室数的不同并不是由fasciated编码序列的差异引起的。Gateway技术是一种快速高效的植物表达载体构建方法。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2018-01-10
基金项目:福建省自然科学基金面上项目(编号:2015J01609)。
作者简介:刘爽(1983—),女,辽宁桓仁人,博士,讲师,从事设施蔬菜栽培生理与生物技术研究。E-mail:liushuangsyau@aliyun.com。
更新日期/Last Update: 2018-10-20