|本期目录/Table of Contents|

[1]齐帆,范兴,陈义,等.基于RNA-seq数据发掘响应干旱的谷子C2H2型锌指蛋白基因[J].江苏农业科学,2019,47(02):41-45.
　Qi Fan,et al.Identification of millet C2H2-type zinc finger protein genes in response to drought stress based on RNA-seq data[J].Jiangsu Agricultural Sciences,2019,47(02):41-45.
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基于RNA-seq数据发掘响应干旱的谷子
C2H2型锌指蛋白基因(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第47卷
期数:
2019年第02期
页码:
41-45
栏目:
生物技术
出版日期:
2019-01-20

文章信息/Info

Title:
Identification of millet C2H2-type zinc finger protein genes in response to drought stress based on RNA-seq data
作者:
齐帆;  范兴;  陈义;  王晶慧;  许慧晶;  马芳芳
山西农业大学农学院，山西太谷 030801
Author(s):
Qi Fan; et al
关键词:
谷子; C2H2型锌指蛋白; 干旱胁迫; 差异表达基因
Keywords:
-
分类号:
S515.01
DOI:
-
文献标志码:
A
摘要:
C2H2型锌指蛋白在植物响应逆境胁迫以及激素信号转导过程中起着重要作用，为了发掘响应干旱胁迫的谷子C2H2型锌指蛋白基因(SiC2H2)，利用转录组测序技术构建谷子聚乙二醇(PEG)处理前后转录组文库，根据表达谱数据鉴定出11个SiC2H2差异表达基因，对其进行理化性质、启动子元件等部分生物信息学分析和进化树及基因结构分析。实时荧光定量逆转录PCR(RT-PCR)验证结果表明，随机选取的4个基因在PEG胁迫前后的表达特点与表达谱数据基本一致。研究结果为后续进一步研究C2H2型锌指蛋白在谷子抗旱性中的作用以及谷子抗旱分子育种提供了理论依据。
Abstract:
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参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2018-02-11
基金项目：国家自然科学基金(编号：31401396)；地方高校国家级大学生创新创业训练计划(编号：201610113023)；山西省青年科技研究基金(编号：201601D021125、2015021142)；山西农大校基金(编号：2013YJ04、2014022、2014YZ2-5)。
作者简介：齐帆(1996—)，女，陕西宝鸡人，研究方向为制药工程。E-mail：1910511457@qq.com。
通信作者：马芳芳，博士，副教授，主要从事植物逆境生理与分子生物学研究。Tel：(0354)6288344；E-mail：mff1984abc@163.com。
更新日期/Last Update: 2019-01-20