|本期目录/Table of Contents|

[1]王超,陈刘军,田伟,等.高通量测序解析微生物肥料对红壤有机农田土壤微生物群落的影响[J].江苏农业科学,2019,47(02):272-277.
 Wang Chao,et al.Study on effect of microbial fertilizer on microbial community in red soil of organic farm via high-throughput sequencing technology[J].Jiangsu Agricultural Sciences,2019,47(02):272-277.
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高通量测序解析微生物肥料对红壤有机农田
土壤微生物群落的影响
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第47卷
期数:
2019年第02期
页码:
272-277
栏目:
资源与环境
出版日期:
2019-01-20

文章信息/Info

Title:
Study on effect of microbial fertilizer on microbial community in red soil of organic farm via high-throughput sequencing technology
作者:
王超1 陈刘军2 田伟1 黄思杰1 王磊1 席运官1
1.环境保护部南京环境科学研究所,江苏南京 210042; 2.南京本源生态农业科技有限公司,江苏南京 211808
Author(s):
Wang Chaoet al
关键词:
微生物肥料根区土壤Illumina Miseq高通量测序微生物群落种群多样性相对丰度
Keywords:
-
分类号:
S144
DOI:
-
文献标志码:
A
摘要:
高通量测序技术可用于精确分析土壤微生物群落,从微生物群落结构和多样性的角度阐释微生物肥料对有机农田根区土壤微生物群落的影响。在红壤有机农田轮作种植条件下,施用微生物肥料后利用Illumina MiSeq高通量测序技术结合相关生物信息学分析土壤细菌和真菌的多样性指数及群落结构。结果表明,从6个有机农田根区土壤样本中获得7 729个细菌分类操作单元(operational taxonomic units,简称OTU)和3 271个真菌OTU,细菌和真菌文库测序覆盖率均在99%以上。微生物肥料会显著降低土壤细菌和真菌种群多样性,且可在一定程度上降低细菌群落丰富度,显著降低真菌群落丰富度;并减少根区土壤特有细菌和真菌物种数量。放线菌门、变形菌门和酸杆菌门是优势细菌,子囊菌门是优势真菌;微生物肥料会提高放线菌门、变形菌门、厚壁菌门和担子菌门的相对丰度,分别比对照组提高29.46%、9.17%、129.33%、165.73%;但会降低酸杆菌门、绿弯菌门、子囊菌门、接合菌门的相对丰度,分别比对照组降低30.14%、33.50%、17.27%、86.33%。因此,施用微生物肥料可改变红壤有机农田根区土壤细菌和真菌的丰富度和多样性,有助于控制作物病害发生。
Abstract:
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备注/Memo

备注/Memo:
收稿日期:2018-04-28
基金项目:云南省环保公益专项(编号:45014);中央级公益性科研院所基本科研业务专项(编号:GYZX170307)。
作者简介:王超(1989—),男,山东临沂人,博士,主要从事有机种植病虫草害防治、土壤健康及相关机理研究。Tel:(025)85287062;E-mail:wcofrcc@126.com。
通信作者:席运官,博士,研究员,主要从事生态农业等研究。E-mail:xygofrcc@126.com。
更新日期/Last Update: 2019-01-20