|本期目录/Table of Contents|

[1]袁锐,方苹,倪金俤,等.江苏地区1株锦鲤疱疹病毒的鉴定[J].江苏农业科学,2019,47(12):200-203.
　Yuan Rui,et al.Isolation and identification of a koi herpesvirus from Jiangsu Province[J].Jiangsu Agricultural Sciences,2019,47(12):200-203.
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江苏地区1株锦鲤疱疹病毒的鉴定(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第47卷
期数:
2019年第12期
页码:
200-203
栏目:
畜牧兽医与水产蚕桑
出版日期:
2019-07-10

文章信息/Info

Title:
Isolation and identification of a koi herpesvirus from Jiangsu Province
作者:
袁锐;  方苹;  倪金俤;  刘训猛;  陈静;  吴亚锋;  王晶晶;  陈辉
江苏省水生动物疫病预防控制中心，江苏南京 210036
Author(s):
Yuan Rui; et al
关键词:
锦鲤; 锦鲤疱疹病毒; 病毒鉴定; 系统进化分析
Keywords:
-
分类号:
S941.41+2
DOI:
-
文献标志码:
A
摘要:
对我国江苏地区养殖锦鲤暴发性鲤疱疹病毒病(koiherpes virus disease，简称KHVD)的病原进行鉴定。2018年5月，江苏省一锦鲤养殖场暴发不明原因传染疾病，病鱼肝、脾、肾脏中未分离到细菌。透射电镜观察发现大量球状病毒粒子。提取自然发病鱼的鳃、肾、脾、肝组织DNA作为模板进行PCR检测，结果显示为阳性。Blast比对结果显示，扩增序列与锦鲤疮疹病毒(koi herpesvirus，简称KHV)胸苷激酶(thymidine kinase，简称TK)基因和KHV DNA聚合酶(SPH)基因核苷酸序列同源性为99%。对病毒株的TK基因全长序列进行系统发育分析，证实该毒株属于KHV亚洲型毒株。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2018-11-06
基金项目：2018年农业部水生动物重大疫病监测与防治项目。
作者简介：袁锐(1986—)，男，江苏南京人，硕士研究生，工程师，研究方向为水生动物病害。E-mail：yr8624@163.com。
通信作者：陈辉，研究员，研究方向为水生动物病害。E-mail：Chenhuijsbf@163.com。
更新日期/Last Update: 2019-06-20