|本期目录/Table of Contents|

[1]钱兰华,钱玮,沈雪林,等.耐盐促生菌的筛选、鉴定及其对黄瓜的促生作用[J].江苏农业科学,2019,47(18):160-163.
 Qian Lanhua,et al.Screening and identification of salt-tolerant bacteria and its promoting effect on cucumber[J].Jiangsu Agricultural Sciences,2019,47(18):160-163.
点击复制

耐盐促生菌的筛选、鉴定及其对黄瓜的促生作用(PDF)
分享到:

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第47卷
期数:
2019年第18期
页码:
160-163
栏目:
园艺与林学
出版日期:
2019-10-15

文章信息/Info

Title:
Screening and identification of salt-tolerant bacteria and its promoting effect on cucumber
作者:
钱兰华1 钱玮2 沈雪林3 胡翠英2 刘乾2 蒋晨威2 朱昫飏2 王桃云2
1.苏州农业职业技术学院,江苏苏州 215008; 2.苏州科技学院化学生物与材料工程学院,江苏苏州 215009;
3.苏州市种子管理站,江苏苏州 215011
Author(s):
Qian Lanhuaet al
关键词:
耐盐分离筛选鉴定巨大芽孢杆菌黄瓜促生作用
Keywords:
-
分类号:
S154. 39
DOI:
-
文献标志码:
A
摘要:
为获得耐盐促生菌株并明确其促生特性,通过耐盐试验和ACC脱氨酶活性对沿海滩涂盐碱地土壤中的微生物进行分离筛选,得到5株耐盐菌株,其中B7的耐盐活性最强。接着采用平皿法和盆栽法研究了盐胁迫下黄瓜种子发芽及盆栽试验中B7对黄瓜的耐盐促生作用。同时对B7的多种促生能力进行测定,最后通过菌株形态、生理生化特征测定及其16S rDNA序列鉴定出B7菌株为巨大芽孢杆菌(Bacillus megaterium)。研究结果表明,该菌株能明显提高黄瓜耐盐促生能力,同时具有产吲哚乙酸(IAA)、产氨、固氮及解磷等多种促生能力,因而具有广泛的应用前景。
Abstract:
-

参考文献/References:

[1]钮旭光,韩梅,宋立超,等. 翅碱蓬内生细菌鉴定及耐盐促生作用研究[J]. 沈阳农业大学学报,2011,42(6):698-702.
[2]张兆林. 浅谈曹妃甸区盐碱地的开发与利用[J]. 中国科技博览,2014(32):325-325.
[3]马晨,马履一,刘太祥,等. 盐碱地改良利用技术研究进展[J]. 世界林业研究,2010,23(2):28-32.
[4]Singh J S. Plant growth promoting rhizobacteria[J]. Resonance,2013,18(3):275-281.
[5]康贻军,程洁,梅丽娟,等. 植物根际促生菌的筛选及鉴定[J]. 微生物学报,2010,50(7):853-861.
[6]曾庆飞,王茜,陆瑞霞,等. 大豆根际促生菌的分离筛选及其对大豆和百脉根生长与品质的影响[J]. 草业学报,2017,26(1):99-111.
[7]郑文波,申飞,闫小梅,等. 红壤中产吲哚乙酸并具解磷作用的促生菌筛选鉴定及促生效果研究[J]. 土壤,2015,47(2):361-368.
[8]Patel V R,Bhatt N. Isolation,development and identification of salt-tolerant bacterial consortium from crude-oil-contaminated soil for degradation of di-azo dye Reactive Blue 220[J]. Water Science and Technology,2015,72(2):311-321.
[9]赵龙飞,徐亚军,常佳丽,等. 具ACC脱氨酶活性大豆根瘤内生菌的筛选、抗性及促生作用[J]. 微生物学报,2016,56(6):1009-1021.
[10]Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemistry,1976,72(1/2):248-254.
[11]Saleh S S,Glick B R. Involvement of gacS and rpoS in enhancement of the plant growth-promoting capabilities of Enterobacter cloacae CAL2 and UW4[J]. Canadian Journal of Microbiology,2001,47(8):698-705.
[12]杨敬辉,文平兰,庄义庆. 颉颃细菌的筛选及生防潜能评估[J]. 西南农业学报,2013,26(2):565-571.
[13]Ribeiro C M,Cardoso E J. Isolation,selection and characterization of root-associated growth promoting bacteria in Brazil Pine (Araucaria angustifolia)[J]. Microbiological Research,2012,167(2):69-78.
[14]Piromyou P,Buranabanyat B,Tantasawat P A,et al. Effect of plant growth promoting rhizobacteria (PGPR) inoculation on microbial community structure in rhizosphere of forage corn cultivated in Thailand[J]. European Journal of Soil Biology,2011,47(1):44-54.
[15]Machuca A,Milagres A M. Use of CAS-agar plate modified to study the effect of different variables on the siderophore production by Aspergillus[J]. Letters in Applied Microbiology,2003,36(3):177-181.
[16]Wichner S,Libbert E. Interactions between plants and epiphytic bacteria regarding their auxin metabolism. Ⅰ. Detection of IAA-producing epiphytic bacteria and their role in long duration experiments on tryptophan metabolism in plant homogenates[J]. Physiologia Plantarum,2010,21(1):227-241.
[17]吴翔,甘炳成,黄忠乾,等. 一株产IAA菌株的筛选、鉴定及培养条件优化[J]. 四川农业大学学报,2014,32(4):432-435,461.
[18]东秀珠,蔡妙英. 常见细菌系统鉴定手册[M]. 北京:科学出版社,2001:353-412.
[19]布坎南R E,吉本斯N E. 伯杰细菌鉴定手册[M]. 北京:科学出版社,1984:195-196.

相似文献/References:

[1]冯 艳,周 蓉,付 蓉,等.玉米遗传多样性分析及耐盐种质筛选[J].江苏农业科学,2015,43(07):59.
 Feng Yan,et al.Analysis of genetic diversity and screening of salt-tolerant germplasm in maize[J].Jiangsu Agricultural Sciences,2015,43(18):59.
[2]李强,马代夫,李秀英,等.高淀粉甘薯品种徐薯29的选育与栽培技术[J].江苏农业科学,2015,43(01):96.
 Li Qiang,et al.Breeding and cultivation techniques of sweet potato cultivar “Xushu 29” with high starch[J].Jiangsu Agricultural Sciences,2015,43(18):96.
[3]李春宏,张培通,郭文琦,等.耐盐甜高粱新品种中科甜3号的选育及栽培技术[J].江苏农业科学,2015,43(03):95.
 Li Chunhong,et al.Breeding and cultivation techniques of new sweet sorghum cultivar “Zhongketian No.3”[J].Jiangsu Agricultural Sciences,2015,43(18):95.
[4]龙卫华,浦惠明,胡茂龙,等.甘蓝型杂交油菜新品种宁杂31的选育[J].江苏农业科学,2016,44(11):114.
 Long Weihua,et al.Breeding of new hybrid rapeseed cultivar “Ningza 31”[J].Jiangsu Agricultural Sciences,2016,44(18):114.
[5]常征,王蓉,李洪潮,等.菌株YS(r)-19分离鉴定及对三七根腐病菌的抑菌活性[J].江苏农业科学,2017,45(06):92.
 Chang Zheng,et al.Isolation and identification of YS(r)-19 strain and its antifungal activity of antagonistic bacteria against Panax notoginseng root rot disease[J].Jiangsu Agricultural Sciences,2017,45(18):92.
[6]张杰,张丽丽,李媛媛,等.1株Cr(Ⅵ)抗性菌株的筛选鉴定及去除Cr(Ⅵ)特性[J].江苏农业科学,2017,45(16):268.
 Zhang Lili,et al.Screening, identification and Cr (Ⅵ) removal characteristics of a Cr(Ⅵ)-resistant strain[J].Jiangsu Agricultural Sciences,2017,45(18):268.
[7]毛涵,陈婧,代飞,等.中华补血草内生真菌发酵物的抑菌和抗氧化活性[J].江苏农业科学,2018,46(08):166.
 Mao Han,et al.Antibacterial and antioxidant activities of endophytic fungi fermentation products of Limonium sinense[J].Jiangsu Agricultural Sciences,2018,46(18):166.
[8]王江英,赵统利,邵小斌,等.日光温室盆栽非洲菊栽培基质筛选及耐盐性分析[J].江苏农业科学,2018,46(23):147.
 Wang Jiangying,et al.Screening of pot cultivation substrate for Gerbera jamesonii in greenhouse and analysis of its salt tolerance[J].Jiangsu Agricultural Sciences,2018,46(18):147.
[9]谢海伟,冯嘉琪,付晓晴,等.药用植物内生真菌的研究进展[J].江苏农业科学,2020,48(14):1.
 Xie Haiwei,et al.Research progress of endophytic fungi in medicinal plants[J].Jiangsu Agricultural Sciences,2020,48(18):1.
[10]付丽,刘加珍,陶宝先,等.盐生植物对盐渍土壤环境的适应机制研究综述[J].江苏农业科学,2021,49(15):32.
 Fu Li,et al.Adaptive mechanism of halophytes to saline soil environment: a review[J].Jiangsu Agricultural Sciences,2021,49(18):32.

备注/Memo

备注/Memo:
收稿日期:2018-05-31
基金项目:江苏省苏州市科技计划项目-应用基础研究计划(编号:SYN201525)。
作者简介:钱兰华(1976—),女,江苏兴化人,副教授,主要从事植物病虫害综合防治研究。E-mail:lanhuaqian@126.com。
通信作者:王桃云,博士,副教授,主要从事植物、微生物资源研究。E-mail:wangtaoyun@usts.edu.cn。
更新日期/Last Update: 2019-09-20