|本期目录/Table of Contents|

[1]山溪,秦文斌,张振超,等.甘蓝蔗糖磷酸合酶家族的鉴定和表达分析[J].江苏农业科学,2021,49(16):53-60.
 Shan Xi,et al.Genome-wide identification and expression analysis of sucrose phosphate synthase family in cabbage[J].Jiangsu Agricultural Sciences,2021,49(16):53-60.
点击复制

甘蓝蔗糖磷酸合酶家族的鉴定和表达分析(PDF)
分享到:

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第49卷
期数:
2021年第16期
页码:
53-60
栏目:
生物技术
出版日期:
2021-08-20

文章信息/Info

Title:
Genome-wide identification and expression analysis of sucrose phosphate synthase family in cabbage
作者:
山溪秦文斌张振超姚悦梅戴忠良饶斌
江苏丘陵地区镇江农业科学研究所,江苏句容 212400
Author(s):
Shan Xiet al
关键词:
蔗糖磷酸合酶甘蓝低温胁迫BoSPSs基因基因表达分析
Keywords:
-
分类号:
S188+.3
DOI:
-
文献标志码:
A
摘要:
蔗糖磷酸合酶(SPS)是调节植物蔗糖生物合成的关键酶。SPS由不同的基因编码组成,具有不同的表达模式和功能差异。利用拟南芥SPS蛋白保守结构域在甘蓝全基因组共鉴定到6个甘蓝SPS家族成员。进化分析结果表明甘蓝SPS基因分为3个亚族。6个BoSPSs家族成员被定位在甘蓝的5条染色体上。启动子顺式作用元件分析结果表明,BoSPSs含有许多与激素、逆境和光响应相关的顺式作用元件。RNA-Seq结果表明,BoSPSA1a在各个组织中均具有较高的表达量,BoSPSB除了在芽中表达量较高,在其他各个器官/组织中表达量均较低;冷敏甘蓝(CS-D9)和耐冷甘蓝(CT-923)中BoSPSA1b低温处理后均上调表达,且不同时间点耐冷甘蓝中的表达量均明显高于冷敏甘蓝。本研究结果有助于了解甘蓝SPS家族的信息,增加了对这些基因在甘蓝生长发育过程中及低温胁迫中所起的作用的理解。
Abstract:
-

参考文献/References:

[1]白蓓蓓,陈业渊,王鹏. 植物蔗糖合酶家族基因鉴定及序列和进化分析[J]. 分子植物育种,2018,16(7):2175-2179.
[2]Baxter C J,Foyer C H,Turner J,et al. Elevated sucrose-phosphate synthase activity in transgenic tobacco sustains photosynthesis in older leaves and alters development[J]. Journal of Experimental Botany,2003,54(389):1813-1820.
[3]Lunn J E. Sucrose-phosphatase gene families in plants[J]. Gene,2003,303:187-196.
[4]Chen S,Hajirezaei M,Bornke F. Differential expression of sucrose-phosphate synthase isoenzymes in tobacco reflects their functional specialization during dark-governed starch mobilization in source leaves[J]. Plant Physiology,2005,139(3):1163-1174.
[5]Lutfiyya L L,Xu N,Dordine R L,et al. Phylogenetic and expression analysis of sucrose phosphate synthase isozymes in plants[J]. Journal of Plant Physiology,2007,164(7):923-933.
[6]Huber S C,Huber J L. Role and regulation of sucrose-phosphate synthase in higher plants[J]. Annual Review of Plant Physiology and Plant Molecular Biology,1996,47:431-444.
[7]Doehlert D C,Huber S C. The role of sulhydyl groups in the regulation of spinach leaf sucrose phosphate synthase [J]. Biochimica Biophysica Acta,1985,93:353-355.
[8]Chen S,Hajirezaei M,Peisker M,et al. Decreased sucrose-6-phosphate phosphatase level in transgenic tobacco inhibits photosynthesis,alters carbohydrate partitioning,and reduces growth[J]. Planta,2005,221(4):479-492.
[9]Grof C P L,So C,Perroux J M,et al. Research note:the five families of sucrose-phosphate synthase genes in Saccharum spp. are differentially expressed in leaves and stem[J]. Functional Plant Biology,2006,33(6):605-610.
[10]Castleden C K,Aoki N,Gillespie V J,et al. Evolution and function of the sucrose-phosphate synthase gene families in wheat and other grasses[J]. Plant Physiology,2004,135(3):1753-1764.
[11]Sun J,Zhang J,Larue C T,et al. Decrease in leaf sucrose synthesis leads to increased leaf starch turnover and decreased RuBP regeneration-limited photosynthesis but not Rubisco-limited photosynthesis in Arabidopsis null mutants of SPSA1[J]. Plant,Cell & Environment,2011,34(4):592-604.
[12]Jiang J,Zhang Z,Cao J. Pollen wall development:the associated enzymes and metabolic pathways[J]. Plant Biology,2013,15(2):249-263.
[13]杨丽梅,方智远,庄木,等. “十二五”我国甘蓝遗传育种研究进展中国蔬菜[J]. 中国蔬菜,2016(11):1-6.
[14]Wang X W,Wang H,Wang J,et al. The genome of the mesopolyploid crop species Brassica rapa[J]. Nature Genetics,2011,43(10):1035-1039.
[15]Liu S Y,Liu Y M,Yang X H,et al. The Brassica oleracea genome reveals the asymmetrical evolution of polyploid genomes[J]. Nature Communications,2014,5:3930.
[16]Cai X,Wu J,Liang J L,et al. Improved Brassica oleracea JZS assembly reveals significant changing of LTR-RT dynamics in different morphotypes[J]. Theoretical and Applied Genetics,2020,133(7):3187-3199.
[17]强毅.植物蔗糖磷酸合成酶的生物信息学分析[J]. 现代生物医学进展,2007,7(4):557-560,570.
[18]Artimo P,Jonnalagedda M,Arnold K A,et al. ExPASy:SIB bioinformatics resource portal[J]. Nucleic Acids Research,2012,40(1):W597-W603.
[19]Chen C J,Xia R,Chen H,et al. TBtools,a toolkit for biologists integrating various HTS-data handling tools with a user-friendly interface [J]. BioRxiv,2018:289660.
[20]Jiang S Y,Chi Y H,Wang J Z,et al. Sucrose metabolism gene families and their biological functions[J]. Scientific Reports,2015,5:17583.
[21]吕佳红,王英珍,程瑞,等. 梨蔗糖合成相关酶SUS和SPS基因家族的鉴定与表达分析[J]. 园艺学报,2018,45(3):421-435.
[22]Wang D,Zhao J,Hu B,et al. Identification and expression profile analysis of the sucrose phosphate synthase gene family in Litchi chinensis Sonn[J]. PeerJ,2018,6:e4379.
[23]魏清江,马张正,勒思,等. 柑橘磷酸蔗糖合酶基因CsSPS的鉴定和表达[J]. 园艺学报,2020,47(2):334-344.
[24]晁毛妮,胡海燕,付丽娜,等. 陆地棉蔗糖磷酸合成酶基因家族的鉴定及表达分析[J]. 棉花学报,2020,32(1):30-41.
[25]Ma P,Zhang X,Chen L,et al. Comparative analysis of sucrose phosphate synthase (SPS) gene family between Saccharum officinarum and Saccharum spontaneum[J]. BMC Plant Biology,2020,20(1):422.
[26]Xu G X,Guo C C,Shan H Y,et al. Divergence of duplicate genes in exon-intron structure[J]. Proceedings of the National Academy of Sciences of the United States of America,2012,109(4):1187-1192.
[27]张莉,荐红举,杨博,等. 甘蓝型油菜蔗糖磷酸合酶(SPS)基因家族成员鉴定及表达分析[J]. 作物学报,2018,44(2):197-207.
[28]Huang D L,Qin C X,Gui Y Y,et al. Role of the SPS gene families in the regulation of sucrose accumulation in sugarcane[J]. Sugar Tech,2017,19(2):117-124.
[29]Chavez A T,Valdez J J,Martinez M,et al. Tissue-specific and developmental pattern of expression of the rice sps1 gene [J]. Plant Physiology,2000,124:641-653.
[30]Okamura M,Aoki N,Hirose T,et al. Tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family in rice[J]. Plant Science,2011,181(2):159-166.
[31]Gibon Y,Blaesing O E,Hannemann J,et al. A robot-based platform to measure multiple enzyme activities in Arabidopsis using a set of cycling assays:comparison of changes of enzyme activities and transcript levels during diurnal cycles and in prolonged darkness[J]. The Plant Cell,2004,16(12):3304-3325.
[32]李会霞,祝令成,张钊,等. 苹果中磷酸蔗糖合酶家族基因的表达特性及其与蔗糖含量的关系[J]. 西北植物学报,2017,37(5):872-878.

相似文献/References:

[1]潘永飞,陈智超,潘跃平.不同类型甘蓝制种父母本定植比例试验[J].江苏农业科学,2014,42(12):214.
 Pan Yongfei,et al.Planting proportion test of different types of cabbage parents during seed production[J].Jiangsu Agricultural Sciences,2014,42(16):214.
[2]万云龙.优质水稻—春甘蓝轮作高效栽培模式[J].江苏农业科学,2014,42(12):90.
 Wan Yunlong.Efficient cultivation mode of high quality rice-spring cabbage rotation[J].Jiangsu Agricultural Sciences,2014,42(16):90.
[3]王志春.防虫网在无公害甘蓝生产上的应用技术[J].江苏农业科学,2015,43(12):178.
 Wang Zhichun.Application of insect-proof screen in pollution-free cabbage production[J].Jiangsu Agricultural Sciences,2015,43(16):178.
[4]郑子松,王林闯,李刚,等.木薯渣复配基质在甘蓝育苗上的应用效果[J].江苏农业科学,2013,41(10):108.
 Zheng Zisong,et al.Application effect of cassava residue compound matrix on cultivation of Brassica oleracea seedlings[J].Jiangsu Agricultural Sciences,2013,41(16):108.
[5]潘永飞,戴忠良,毛忠良,等.甘蓝制种低产的主要原因及对策[J].江苏农业科学,2013,41(10):110.
 Pan Yongfei,et al.Main reasons and countermeasures for low yield of cabbage seeds production[J].Jiangsu Agricultural Sciences,2013,41(16):110.
[6]宋国春,于建垒,李瑞娟,等.虱螨脲在甘蓝和土壤中的残留及安全性评价[J].江苏农业科学,2013,41(11):334.
 Song Guochun,et al.Residue and safety evaluation of lufenuron in Brassica oleracea and soils[J].Jiangsu Agricultural Sciences,2013,41(16):334.
[7]曾爱松,高兵,宋立晓,等.秋季专用牛心甘蓝新品种锦秋55的选育[J].江苏农业科学,2015,43(11):230.
 Zeng Aisong,et al.Breeding of autumn special oxheart cabbage cultivar “Jinqiu 55”[J].Jiangsu Agricultural Sciences,2015,43(16):230.
[8]毕云飞,苏小俊,蒋芳玲,等.外源水杨酸对高温胁迫下甘蓝幼苗生长及生理特性的影响[J].江苏农业科学,2015,43(08):133.
 Bi Yunfei,et al.Effects of exogenous salicylic acid on growth and physiological characteristics of cabbage seedlings under the stress of high temperature[J].Jiangsu Agricultural Sciences,2015,43(16):133.
[9]潘永飞,戴忠良,潘跃平.甘蓝制种不同定植密度及定植方式试验[J].江苏农业科学,2015,43(08):146.
 Pan Yongfei,et al.Experimental study on different planting density and ways of Brassica oleracea seed production[J].Jiangsu Agricultural Sciences,2015,43(16):146.
[10]苏 南,苏小俊,蒋芳玲,等.表油菜素内酯对高温胁迫下甘蓝幼苗抗逆性的影响[J].江苏农业科学,2015,43(07):137.
 Su Nan,et al.Effect of epiBR on stress resistance of cabbage seedlings under high temperature stress[J].Jiangsu Agricultural Sciences,2015,43(16):137.

备注/Memo

备注/Memo:
收稿日期:2020-12-28
基金项目:江苏省重点研发计划(编号:BE2020403);镇江市科技计划(编号:NY2020001)。
作者简介:山溪(1990—),女,河南洛阳人,硕士,助理研究员,主要从事甘蓝类蔬菜遗传育种研究。E-mail:saiwaicanxue1990@163.com。
通信作者:戴忠良,硕士,研究员,主要从事甘蓝类蔬菜遗传育种研究。E-mail:daizhongliang2008@126.com。
更新日期/Last Update: 2021-08-20