|本期目录/Table of Contents|

[1]章明,单艳菊,姬改革,等.PRKAG3基因在鸡不同部位肌肉中的表达及其与肌纤维类型的相关性[J].江苏农业科学,2021,49(16):144-147.
 Zhang Ming,et al.Expression of RKAG3 gene in different parts of muscle and its association with myofiber type in chicken[J].Jiangsu Agricultural Sciences,2021,49(16):144-147.
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PRKAG3基因在鸡不同部位肌肉中的表达及其与肌纤维类型的相关性(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第49卷
期数:
2021年第16期
页码:
144-147
栏目:
畜牧兽医与水产蚕桑
出版日期:
2021-08-20

文章信息/Info

Title:
Expression of RKAG3 gene in different parts of muscle and its association with myofiber type in chicken
作者:
章明单艳菊姬改革巨晓军刘一帆屠云洁邹剑敏束婧婷
江苏省家禽科学研究所/江苏省家禽遗传育种重点实验室,江苏扬州 225125
Author(s):
Zhang Minget al
关键词:
PRKAG3基因肌纤维类型表达相关性肌肉AMPK
Keywords:
-
分类号:
S831.2
DOI:
-
文献标志码:
A
摘要:
PRKAG3是影响畜禽肉品质的关键基因。为研究PRKAG3基因在鸡不同部位肌肉中的表达及其与肌纤维类型的相关性,以隐性白羽肉鸡为研究对象,用ATPase碱孵育法对不同部位肌肉肌纤维类型进行分析,采用实时荧光定量PCR法检测PRKAG3基因在这些肌肉组织中的表达并将其与肌纤维类型进行关联分析。结果表明,PRKAG3基因在Ⅰ型肌纤维比例相对较高的比目鱼肌和缝匠肌中表达较低,在Ⅱa型比例较高的髂胫外侧肌、腓骨长肌以及只含有Ⅱb型肌纤维的胸大肌、胸小肌中表达较高,且差异显著(P<0.05)。相关性分析结果显示,肌肉中PRKAG3基因的表达与Ⅰ型肌纤维比例呈显著负相关(P<0.05),与Ⅱa型和Ⅱb型肌纤维比例均呈正相关,但差异不显著(P>005)。提示,在鸡骨骼肌中PRKAG3很可能是AMPK在白肌中发挥作用的主要γ调节亚基。
Abstract:
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备注/Memo

备注/Memo:
收稿日期:2020-11-06
基金项目:现代农业产业技术体系建设专项(编号:CARS-41);江苏现代农业(肉鸡)产业技术体系集成创新中心(编号:JATS[2020]356);江苏省农业重大新品种创制项目(编号:PZCZ201728);江苏省属公益类科研院所自主科研经费(编号:BM2018026);江苏省农业科技自主创新资金[编号:CX(20)3003、CX(18)3003]。
作者简介:章明(1978—),男,江苏泰州人,硕士,副研究员,主要从事家禽遗传育种研究,E-mail:zm@jips.com;共同第一作者:单艳菊(1982—),女,江苏盐城人,硕士,副研究员,主要从事家禽遗传育种研究,E-mail:shanyanjusyj@163.com。
通信作者:束婧婷,博士,副研究员,主要从事家禽遗传育种研究。E-mail:shujingting@163.com。
更新日期/Last Update: 2021-08-20