[1]梁玉镯,陈新娜,陈东亮,等.MYB转录因子调控植物花青素生物合成研究进展[J].江苏农业科学,2022,50(22):55-64.
　Liang Yuzhuo,et al.Research progress on MYB transcription factor regulating plant anthocyanin biosynthesis[J].Jiangsu Agricultural Sciences,2022,50(22):55-64.
点击复制

MYB转录因子调控植物花青素生物合成研究进展()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第50卷
期数:
2022年第22期
页码:
55-64
栏目:
专论与综述
出版日期:
2022-11-20

文章信息/Info

Title:
Research progress on MYB transcription factor regulating plant anthocyanin biosynthesis
作者:
梁玉镯1; 2; 3; 陈新娜1; 2; 3; 陈东亮2; 3; 王森1; 2; 3; 李彦慧1; 黄丛林2; 3
1.河北农业大学园林与旅游学院，河北保定 071000； 2.北京市农林科学院草业花卉与景观生态研究所，北京 100097；3.北京市功能花卉工程技术研究中心，北京 100097
Author(s):
Liang Yuzhuo; et al
关键词:
花青素; 生物合成; 结构基因; MYB; 表达调控
Keywords:
-
分类号:
S188
DOI:
-
文献标志码:
A
摘要:
花青素是植物中广泛存在的一类水溶性天然色素，是许多植物叶片、果实、花等器官呈现丰富颜色的主要呈色物质，并广泛参与植物抵御低温、干旱等非生物胁迫及病害、虫害等各种生物胁迫的生化进程。MYB是植物中最大的转录因子家族之一，广泛参与植物的细胞形态建成、次生代谢物生物合成、分生组织形成、抵御生物及非生物胁迫等众多生理生化进程，同时也是植物花青素生物合成过程中最重要的调控因子，直接影响着花青素的种类和含量，影响花的最终呈色。本文综述了植物花青素生物代谢相关研究，总结了植物花青素的基本特征、生物合成的主要途径、涉及的主要结构基因及其功能等；综述了MYB类转录因子的结构特征及其参与植物花青素代谢调控的主要方式，并重点综述了MBW复合体在植物花青素代谢中的重要作用，以期为进一步研究花青素生物合成调控机制、花色设计育种等奠定基础。
Abstract:
-

参考文献/References:

［1］余莉. 蜡梅花挥发性组分与花色色素分析［D］. 武汉：华中农业大学，2013.
［2］Goto T，Kondo T. Structure and molecular stacking of anthocyanins flower color variation［J］. Angewandte Chemie International Edition in English，1991，30(1)：17-33.
［3］Kim J，Lee W J，Vu T T. High accumulation of anthocyanins via the ectopic expression of AtDFR confers significant salt stress tolerance in Brassica napus L.［J］. Plant Cell Reports，2017，36(8)：1215-1224.
［4］An J P，Li R，Qu F J，et al. R2R3-MYB transcription factor MdMYB23 is involved in the cold tolerance and proanthocyanidin accumulation in apple［J］. The Plant Journal，2018，96(3)：562-577.
［5］Naing A H，Ai T N，Lim K B，et al. Overexpression of Rosea1 from snapdragon enhances anthocyanin accumulation and abiotic stress tolerance in transgenic tobacco［J］. Frontiers in Plant Science，2018，9：1070.
［6］Meng X，Yin B，Feng H L，et al. Overexpression of R2R3-MYB gene leads to accumulation of anthocyanin and enhanced resistance to chilling and oxidative stress［J］. Biologia Plantarum，2014，58(1)：121-130.
［7］Li P，Li Y J，Zhang F J，et al. The Arabidopsis UDP-glycosyl transferases UGT79B2 and UGT79B3，contribute to cold，salt and drought stress tolerance via modulating anthocyanin accumulation［J］. The Plant Journal，2017，89(1)：85-103.
［8］Flachowsky H，Szankowski I，Fischer T C，et al. Transgenic apple plants overexpressing the Lc gene of maize show an altered growth habit and increased resistance to apple scab and fire blight［J］. Planta，2010，231(3)：623-635.
［9］Long L，Zhao J R，Xu F C. Silencing of GbANS reduces cotton resistance to Verticillium dahliae through decreased ROS scavenging during the pathogen invasion process［J］. Plant Cell，Tissue and Organ Culture，2018，135(5)：213-221.
［10］Li X，Ouyang X F，Zhang Z S，et al. Over-expression of the red plant gene R1 enhances anthocyanin production and resistance to bollworm and spider mite in cotton［J］. Molecular Genetics & Genomics，2019，294(2)：469-478.
［11］Xu W J，Dubos C，Lepiniec L. Transcriptional control of flavonoid biosynthesis by MYB-bHLH-WDR complexes［J］. Trends in Plant Science，2015，20(3)：176-185.
［12］Kobayashi K，Suzuki T，Iwata E，et al. MYB3Rs，plant homologs of Myb oncoproteins，control cell cycle-regulated transcription and form DREAM-like complexes［J］. Transcription，2015，6(5)：106-111.
［13］陈敏，杨清. 马铃薯花色苷生物合成与调控研究进展［J］. 中国马铃薯，2013，27(4)：232-238.
［14］Winkel-Shirley B. Flavonoid biosynthesis. A colorful model for genetics，biochemistry，cell biology，and biotechnology［J］. Plant Physiology，2001，126(2)：485-493.
［15］Grotewold E. The genetics and biochemistry of floral pigments［J］. Annual Review of Plant Biology，2006，57(1)：761-780.
［16］Kroon J，Souer E，Graaff A D，et al. Cloning and structural analysis of the anthocyanin pigmentation locus Rt of Petunia hybrid：characterization of insertion sequences in two mutant alleles［J］. The Plant Journal，1994，5(1)：69-80.
［17］Holton T A，Cornish E C. Genetics and biochemistry of anthocyanin biosynthesis［J］. Plant Cell，1995，7(7)：1071-1083.
［18］Davies K M. Modifying anthocyanin production in flowers［M］//Gould K，Davies K，Winefield C. Anthocyanins，2009：49-80.
［19］张云洁，潘怡辰，王汝茜，等. 植物花青素生物合成途径相关基因的研究进展［J］. 安徽农业科学，2014，42(34)：12014-12016，12080.
［20］Mehdy M C，Lamb C J. Chalcone isomerase cDNA cloning and mRNA induction by fungal elicitor，wounding and infection［J］. The EMBO Journal，1987，6(6)：1527-1533.
［21］Dong X Y，Braun E L，Grotewold E. Functional conservation of plant secondary metabolic enzymes revealed by complementation of Arabidopsis flavonoid mutants with maize genes［J］. Plant Physiology，2001，127(1)：46-57.
［22］Sun W，Meng X Y，Liang L J，et al. Molecular and biochemical analysis of chalcone synthase from freesia hybrid in flavonoid biosynthetic pathway［J］. PLoS One，2015，10(3)：e0119054.
［23］Spribille R，Forkmann G. Genetic control of chalcone synthase activity in flowers of Antirrhinum majus［J］. Phytochemistry，1982，21(9)：2231-2234.
［24］Hemleben V，Dressel A，Epping B，et al. Characterization and structural features of a chalcone synthase mutation in a white-flowering line of Matthiola incana R. Br.(Brassicaceae)［J］. Plant Molecular Biology，2004，55(3)：455-465.
［25］Lim W S，Li J R. Co-expression of onion chalcone isomerase in Del/Ros1-expressing tomato enhances anthocyanin and flavonol production［J］. Plant Cell，Tissue and Organ Culture，2017，128(1)：113-124.
［26］Tanaka Y，Sasaki N，Ohmiya A. Biosynthesis of plant pigments：anthocyanins，betalains and carotenoids［J］. The Plant Journal，2008，54(4)：733-749.
［27］Miyahara T，Hamada A，Okamoto M，et al. Identification of flavonoid 3′-hydroxylase in the yellow flower of Delphinium zalil［J］. Journal of Plant Physiology，2016，202：92-96.
［28］QReilly C，Shepherd N S，Pereira A，et al. Molecular cloning of the a1 locus of Zea mays using the transposable elements En and Mu1［J］. The EMBO Journal，1985，4(4)：877-882.
［29］Li Y T，Li J N，Chai Y R，et al. Cloning and sequence analysis of a DFR gene from Brassica campestris L. var. oleifera DC［J］. Molecular Plant Breeding，2005，3(4)：485-492.
［30］Lu Q N，Yang Q，Zou H W. Effects of cerium on accumulation of anthocyanins and expression of anthocyanin biosynthetic genes in potato cell tissue cultures［J］. Journal of Rare Earths，2006，24(4)：479-484.
［31］Gong Z Z，Yamazaki M，Sugiyama M，et al. Cloning and molecular analysis of structural genes involved in anthocyanin biosynthesis and expressed in a forma-specific manner in Perilla frutescens［J］. Plant Molecular Biology，1997，35(6)：915-927.
［32］王如凤. 桑树花青素生物合成相关基因及其与桑椹花青素含量的关系研究［D］. 镇江：江苏科技大学，2019.
［33］Nakajima J I，Tanaka Y，Yamazaki M，et al. cDNA cloning and gene expression of anthocyanidin synthase from Torenia fournieri［J］. Plant Biotechnology，2000，17(4)：331-335.
［34］Rosati C，Cadic A，Duron M，et al. Molecular characterization of the anthocyanidin synthase gene in Forsythia×intermedia reveals organ-specific expression during flower development［J］. Plant Science，1999，149(1)：73-79.
［35］Nakatsuka T，Nishihara M，Mishiba K，et al. Two different mutations are involved in the formation of white-flowered gentian plants［J］. Plant Science，2005，169(5)：949-958.
［36］Welch C R，Wu Q L，Simon J E. Recent advances in anthocyanin analysis and characterization［J］. Current Analytical Chemistry，2008，4(2)：75-101.
［37］Andersen  M，Jordheim M. Anthocyanins. In：Encyclopedia of Life Sciences (ELS)［M］. Chichester：John Wiley & Sons，Ltd，2010：1-12.
［38］Nobuhiro S，Toru N. Achievements and perspectives in biochemistry concerning anthocyanin modification for blue flower coloration［J］. Plant & Cell Physiology 2014，56(1)：28-40.
［39］Tanaka Y，Brugliera F，Chandler S. Recent progress of flower colour modification by biotechnology［J］. International Journal of Molecular Sciences，2009，10(12)：5350-5369.
［40］Luo J，Nishiyama Y，Fuell C，et al. Convergent evolution in the BAHD family of acyl transferases：identification and characterization of anthocyanin acyl transferases from Arabidopsis thaliana［J］. The Plant Journal，2007，50(4)：678-695.
［41］Yoshida B，Mori M，Kondo T. Blue flower color development by anthocyanins：from chemical structure to cell physiology［J］. Nat Prod Rep，2009，26(7)：884-915.
［42］Zhou M L，Hou H L，Zhu X M，et al. Soybean transcription factor GmMYBZ2 represses catharanthine biosynthesis in hairy roots of Catharanthus roseus［J］. Applied Microbiology and Biotechnology，2011，91(4)：1095-1105.
［43］Paz-Ares J，Ghosal D，Wienand U，et al. The regulatory c1 locus of Zea mays encodes a protein with homology to myb proto-oncogene products and with structural similarities to transcriptional activators［J］. The EMBO Journal，1987，6(12)：3553-3558.
［44］王雪倩，袁国振，吴泽，等. 百合LaMYB26基因克隆与表达分析［J］. 植物生理学报，2019，55(3)：290-300.
［45］Dubos C，Stracke R，Grotewold E，et al. MYB transcription factors in Arabidopsis［J］. Trends in Plant Science，2010，15(10)：573-581.
［46］左然，徐美玲，柴国华，等. 植物MYB转录因子功能及调控机制研究进展［J］. 生命科学，2012，24(10)：1133-1140.
［47］Jia G Q，Li B，Zhang D F，et al. Cloning and characterization of a novel R1-MYB transcription factor in maize［J］. Progress in Natural Science，2009，19(9)：1089-1096.
［48］Wang Z Y，Tobin E M. Constitutive expression of the CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) gene disrupts circadian rhythms and suppresses its own expression［J］. Cell，1998，93(7)：1207-1217.
［49］Chen Y H，Yang X Y，He K，et al. The MYB transcription factor superfamily of Arabidopsis：expression analysis and phylogenetic comparison with the rice MYB family［J］. Plant Molecular Biology，2006，60(1)：107-124.
［50］Gigolashvili T，Yatusevich R，Berger B，et al. The R2R3-MYB transcription factor HAG1/MYB28 is a regulator of methionine-derived glucosinolate biosynthesis in Arabidopsis thaliana［J］. The Plant Journal，2007，51(2)：247-261.
［51］Ito M. Conservation and diversification of three-repeat Myb transcription factors in plants［J］. Journal of Plant Research，2005，118(1)：61-69.
［52］Haga N，Kato K，Murase M，et al. R1R2R3-MYB proteins positively regulate cytokinesis through activation of KNOLLE transcription in Arabidopsis thaliana［J］. Development，2007，134(6)：1101-1110.
［53］Li H L，Guo D，Peng S Q. Molecular characterization of the Jatropha curcas JcR1MYB1 gene encoding a putative R1-MYB transcription factor［J］. Genetics and Molecular Biology，2014，37(3)： 549-555.
［54］Wang Z Y，Tobin E M. Constitutive expression of the CIRCADIAN CLOCK ASSOCIATED l (CCA1) gene disrupts circadian rhythms and suppresses its own expression［J］. Cell，1998，93(7)： 1207-1217.
［55］万小荣，李玲. 植物的MYB蛋白［J］. 植物生理学通讯，2002，38(2)：165-170.
［56］樊锦涛，蒋琛茜，邢继红，等. 拟南芥R2R3-MYB家族第22亚族的结构与功能［J］. 遗传，2014，36(10)：985-994.
［57］Lepiniec L，Debeaujon I，Routaboul J M，et al. Genetics and biochemistry of seed flavonoids［J］. Annual Review of Plant Biology，2006，57(1)：405-430.
［58］Gonzalez A，Zhao M Z，Leavitt J M，et al. Regulation of the anthocyanin biosynthetic pathway by the TTG1/bHLH/Myb transcriptional complex in Arabidopsis seedlings［J］. Plant Journal，2008，53(5)：814-827.
［59］Stracke R，Ishihara H，Huep G，et al. Differential regulation of closely related R2R3-MYB transcription factors controls flavonol accumulation in different parts of the Arabidopsis thaliana seedling［J］. The Plant Journal，2007，50（4）：660-677.
［60］Zhou M L，Sun Z M，Wang C L，et al. Changing a conserved amino acid in R2R3-MYB transcription repressors results in cytoplasmic accumulation and abolishes their repressive activity in Arabidopsis［J］. The Plant Journal，2015，84(2)：395-403.
［61］Cone K C，Burr F A，Burr B. Molecular analysis of the maize anthocyanin regulatory locus C1［J］. Proceedings of the National Academy of Sciences，1986，83(24)：9631-9635.
［62］Mathews H，Clendennen S K，Caldwell C G，et al. Activation tagging in tomato identifies a transcriptional regulator of anthocyanin biosynthesis，modification，and transport［J］. Plant Cell，2003，15(8)：1689-1703.
［63］Davies K M，Schwinn K E. Transcriptional regulation of secondary metabolism［J］. Functional Plant Biology，2003，30(9)：913-925.
［64］Yuan Y，Chiu L W，Li L. Transcriptional regulation of anthocyanin biosynthesis in red cabbage［J］. Planta，2009，230(6)：1141-1153.
［65］Chiu L W，Zhou X，Burke S，et al. The purple cauliflower arises from activation of a MYB transcription factor［J］. Plant Physiology，2010，154(3)：1470-1480.
［66］Mano H，Ogasawara F，Sato K，et al. Isolation of a regulatory gene of anthocyanin biosynthesis in tuberous roots of purple-fleshed sweet potato［J］. Plant Physiology，2007，143(3)：1252-1268.
［67］Hsu C C，Chen Y Y，Tsai W C，et al. Three R2R3-MYB transcription factors regulate distinct floral pigmentation patterning in Phalaenopsis spp.［J］. Plant Physiology，2015，168(1)：175-191.
［68］Espley R V，Hellens R P，Putterill J，et al. Red colouration in apple fruit is due to the activity of the MYB transcription factor MdMYB10［J］. The Plant Journal，2007，49(3)：414-427.
［69］Chagné D，Wang K L，Espley R V，et al. An ancient duplication of apple MYB transcription factors is responsible for novel red fruit-flesh phenotypes［J］. Plant Physiology，2013，161(1)：225-239.
［70］Ravaglia D，Espley R V，Henry-Kirk R A，et al. Transcriptional regulation of flavonoid biosynthesis in nectarine (Prunus persica) by a set of R2R3MYB transcription factors［J］. BMC Plant Biology，2013，13(1)：68.
［71］Lin W K，Bolitho K，Grafton K，et al. An R2R3 MYB transcription factor associated with regulation of the anthocyanin biosynthetic pathway in Rosaceae［J］. BMC Plant Biology，2010，10(1)：50.
［72］Adam M T，Felix W J，Steele R J，et al. Light-induced expression of a MYB gene regulates anthocyanin biosynthesis in red apples［J］. Plant Physiology，2006，142(3)：1216-1232.
［73］Feng S Q，Wang Y L，Yang S，et al. Anthocyanin biosynthesis in pears is regulated by a R2R3-MYB transcription factor PyMYB10［J］. Planta，2010，232(1)：245-255.
［74］Tohge T，Nishiyama Y，Hirai M Y，et al. Functional genomics by integrated analysis of metabolome and transcriptome of Arabidopsis plants over-expressing an MYB transcription factor［J］. The Plant Journal，2005，42(2)：218-235.
［75］Benzvi M M，Shklarman E，Masci T，et al. PAP1 transcription factor enhances production of phenylpropanoid and terpenoid scent compounds in rose flowers［J］. New Phytologist，2012，195(2)：335-345.
［76］Li X，Gao M J，Pan H Y，et al. Purple canola：Arabidopsis PAP1 increases antioxidants and phenolics in Brassica napus leaves［J］. Journal of Agricultural & Food Chemistry，2010，58(3)：1639.
［77］Yi J X，Michael R D，Li X Y，et al. A single repeat MYB transcription factor，GmMYB176，regulates CHS8 gene expression and affects is of lavonoid biosynthesis in soybean［J］. Plant Journal，2010，62(7)：1019-1034.
［78］Tamagnone L，Merida A，Parr A，et al. The AmMYB308 and AmMYB330 transcription factors from Antirrhinum regulated phenylpropanoid and lignin biosynthesis in transgenic tobacco［J］. The Plant Cell，1998，10(2)：135-154.
［79］Wang L K，Micheletti D，Palmer J，et al. High temperature reduces apple fruit colour via modulation of the anthocyanin regulatory complex［J］. Plant Cell & Environment，2011，34(7)：1176-1190.
［80］Cavallini E，Matus J T，Finezzo L，et al. The phenylpropanoid pathway is controlled at different branches by a set of R2R3-MYB C2 repressors in grapevine［J］. Plant Physiology，2015，167(4)：1448-1470.
［81］刘中帅，袁义杭，张通，等. 蓝莓转录因子VcMYB21在果实着色及幼苗UV处理中的响应［J］. 植物生理学报，2017，53(1)：118-128.
［82］窦立雯. 杨树转录因子MYB93负调控类黄酮和木质素合成的分子机制研究［D］. 重庆：西南大学，2019.
［83］赵海霞，吴小峰，白悦辰，等. 苦荞芽期黄铜合成关键酶和MYB转录因子基因的表达分析［J］. 农业生物技术学报，2012，20(2)：121-128.
［84］Zhu H F，Fitzsimmons K，Khandelwal A，et al. CPC，a single-repeat R3MYB，is a negative regulator of anthocyanin biosynthesis in Arabidopsis［J］. Molecular Plant，2009，2(4)：790-802.
［85］Simon M，Lee M M，Lin Y，et al. Distinct and overlapping roles of single-repeat MYB genes in root epidermal patterning［J］. Development Biology，2007，311(2)：566-578.
［86］Colanero S，Perata P，Gonzali S. The atroviolacea gene encodes an R3-MYB protein repressing anthocyanin synthesis in tomato plants［J］. Frontiers in Plant Science，2018，9：830.
［87］Fu Z Z，Wang L M，Shang H Q，et al. An R3-MYB gene of Phalaenopsis，MYBx1，represses anthocyanin accumulation［J］. Plant Growth Regulation，2019，88(5)：129-138.
［88］Sakai M，Yamagishi M，Matsuyama K. Repression of anthocyanin biosynthesis by R3-MYB transcription factors in lily (Lilium spp.)［J］. Plant Cell Reports，2019，38(5)：609-622.
［89］Chen B，Wang X，Hu Y，et al. Ectopic expression of a c1-I allele from maize inhibits pigment formation in the flower of transgenic tobacco［J］. Molecular Biotechnology，2004，26(3)：187-192.
［90］Springob K，Nakajima J I，Yamazaki M，et al. Recent advances in the biosynthesis and accumulation of anthocyanins［J］. Natural Product Reports，2003，20(3)：288-303.
［91］Wang Y R，Lu Y F，Hao S X，et al. Different coloration patterns between the red-and white-fleshed fruits of malus crabapples［J］. Scientia Horticulturae，2015，194：26-33.
［92］Zhu L，Shan H，Chen S M，et al. The heterologous expression of the Chrysanthemum R2R3-MYB transcription factor CmMYB1 alters lignin composition and represses flavonoid synthesis in Arabidopsis thaliana［J］. PLoS One，2013，8(6)：e65680.
［93］Park J S，Kim J B，Cho K J，et al. Arabidopsis R2R3-MYB transcription factor AtMYB60 functions as a transcriptional repressor of anthocyanin biosynthesis in lettuce (Lactuca sativa)［J］. Plant Cell Reports，2008，27(6)：985-994.
［94］Zifkin M，Jin A，Ozga J A，et al. Gene expression and metabolite profiling of developing high bush blueberry fruit indicates transcriptional regulation of flavonoid metabolism and activation of abscisic acid metabolism［J］. Plant Physiology，2012，158(1)：200-224.
［95］Li P H，Chen B B，Zhang G Y，et al. Regulation of anthocyanin and proanthocyanidin biosynthesis by Medicago truncatula bHLH transcription factor MtTT8［J］. New Phytologist，2016，210(3)：905-921.
［96］Pooma W，Gersos C，Grotewold E. Transposon insertions in the promoter of the Zea mays a1 gene differentially affect transcription by the Myb factors P and C1［J］. Genetics，2002，161(2)：793-801.
［97］Hernandez J M，Heine G F，Irani N G，et al. Different mechanisms participate in the r-dependent activity of the R2R3 MYB transcription factor C1［J］. Journal of Biological Chemistry，2004，279(46)：48205-48213.
［98］叶广继，张波，陈文杰，等. 紫粒小麦高原115中R2R3-MYB转录因子TaMYB3-4A的克隆及功能分析［J］. 分子植物育种，2016，14(8)：1940-1947.
［99］王延谦，张波，陈文杰，等. 小麦R2R3-MYB转录因子TaMYB3-4D的克隆及功能分析［J］. 西北植物学报，2015，35(4)：646-652.
［100］Borevitz J O，Xia Y，Blount J，et al. Activation tagging identifies a conserved MYB regulator of phenylpropanoid biosynthesis［J］. The Plant Cell，2000，12：2383-2393.
［101］Baudry A，Heim M A，Dubreucq B，et al. TT2，TT8，and TTG1 synergistically specify the expression of BANYULS and pro anthocyanidin biosynthesis in Arabidopsis thaliana［J］. Plant Journal，2004，39(3)：366-380.
［102］Liu X F，Yin X R，Allan A C，et al. The role of MrbHLH1 and MrMYB1 in regulating anthocyanin biosynthetic genes in tobacco and Chinese bayberry(Myrica rubra)during anthocyanin biosynthesis［J］. Plant Cell，Tissue and Organ Culture，2013，115(3)：285-298.
［103］Hichri I，Barrieu F，Bogs J，et al. Recent advances in the transcriptional regulation of the flavonoid biosynthetic pathway［J］. Journal of Experimental Botany，2011，62(8)：2465-2483.
［104］Starkevicˇ P，Pauktyte· J，Kazanavicˇiūte· V，et al. Expression and anthocyanin biosynthesis-modulating potential of sweet cherry (Prunus avium L.) MYB10 and bHLH genes［J］. PLoS One，2015，10(5)：e0126991.
［105］Spelt C，Quattrocchio F，Mol J N，et al. Anthocyanin1 of petunia encodes a basic helix-loop-helix protein that directly activates transcription of structural anthocyanin genes［J］. Plant Cell，2000，12(9)：1619-1631.
［106］Guan X Y，Pang M X，Nah G，et al. miR828 and miR858 regulate homoeologous MYB2 gene functions in Arabidopsis trichome and cotton fibre development［J］. Nature Communications，2014，5(1)：3050-3059.
［107］Yang S，Zhang M，Xu L，et al. MiR858b inhibits proanthocyanidin accumulation by the repression of DkMYB19 and DkMYB20in persimmon［J］. Frontiers in Plant Science，2020，11：576378.
［108］Butelli E，Titta L，Giorgio M，et al. Enrichment of tomato fruit with health-promoting anthocyanins by expression of select transcription factors［J］. Nature Biotechnology，2008，26（11）： 1301-1308.
［109］Yang F X，Cai J，Yang Y，et al. Overexpression of microRNA828 reduces anthocyanin accumulation in Arabidopsis［J］. Plant Cell Tissue & Organ Culture，2013，115（2）： 159-167.
［110］Gou J Y，Felippes F F，Liu C J，et al. Negative regulation of anthocyanin biosynthesis in Arabidopsis by a miR156-tar-geted SPL transcription factor［J］. The Plant Cell，2011，23（4）：1512-1522.
［111］Chiou T J. The role of microRNAs in sensing nutrient stress［J］. Plant Cell and Environment，2007，30（3）：323-332.
［112］Gasciolli V，Mallory A C，Bartel D P，et al. Partially redundant functions of Arabidopsis DICER-like enzymes and a role for DCL4 in producing trans-acting siRNAs［J］. Current Biology，2005，15（16）：1494-1500.
［113］Ohno S，Hosokawa M，Kojima M，et al. Simultaneous post-transcriptional gene silencing of two different chalcone synthase genes resulting in pure white flowers in the octoploid dahlia［J］. Planta，2011，234（5）：945-958.
［114］Morita Y，Saito R，Ban Y，et al. Tandemly arranged chalcone synthase A genes contribute to the spatially regulated expression of siRNA and the natural bicolor floral phenotype in Petunia hybrida［J］. The Plant Journal，2012，70（5）：739-749.

相似文献/References:

[1]郑蔚然,胡莉,袁玉伟,等.蓝莓中花青素提取及检测技术的研究进展[J].江苏农业科学,2013,41(06):233.
　Zheng Weiran,et al.Research progress on extraction and detection technology for anthocyanin in blueberries[J].Jiangsu Agricultural Sciences,2013,41(22):233.
[2]孙小龙,付永前.C4二羧酸全生物合成技术的研究现状与展望[J].江苏农业科学,2014,42(12):17.
　Sun Xiaolong,et al.Research status and prospects of C4 dicarboxylic acid total biosynthesis technology[J].Jiangsu Agricultural Sciences,2014,42(22):17.
[3]孙健,岳瑞雪,钮福祥,等.紫甘薯花青素的大孔树脂动态吸附工艺优化[J].江苏农业科学,2013,41(06):227.
　Sun Jian,et al.Optimization of dynamic adsorption technology of macroporous resin for anthocyanin in purple sweet potato[J].Jiangsu Agricultural Sciences,2013,41(22):227.
[4]王倩,刘卫东,朱士农,等.茄子营养品质与主要农艺性状的灰色关联分析[J].江苏农业科学,2015,43(12):184.
　Wang Qian,et al.Grey relational grade analysis between quality and main agronomic traits of eggplant[J].Jiangsu Agricultural Sciences,2015,43(22):184.
[5]彭佳佳,刘克林,刘丽娅,等.红掌佛焰苞数字基因表达谱及关键基因表达水平分析[J].江苏农业科学,2016,44(03):48.
　Peng Jiajia,et al.Digital gene expression profiling and key genes expression analysis of Anthurium andraeanum spathe[J].Jiangsu Agricultural Sciences,2016,44(22):48.
[6]苏丁,蔡位辉,张德巧,等.越橘果实中花青素含量及抗氧化能力分析[J].江苏农业科学,2013,41(11):339.
　Su Ding,et al.Analysis of anthocyanin contents and its antioxidant activity in Vaccinium vitis-idaea fruits[J].Jiangsu Agricultural Sciences,2013,41(22):339.
[7]胡永红,曹峥,杨文革,等.多效霉素研究进展[J].江苏农业科学,2013,41(12):1.
　Hu Yonghong,et al.Research progress of polyoxin[J].Jiangsu Agricultural Sciences,2013,41(22):1.
[8]孙健,王洪云,钮福祥,等.不同品种紫甘薯花青素含量及抗氧化活性差异[J].江苏农业科学,2013,41(12):323.
　Sun Jian,et al.Difference of anthocyanin contents and antioxidant activities of different purple sweet potatoes[J].Jiangsu Agricultural Sciences,2013,41(22):323.
[9]王彩云,张晓东,沈涛,等.龙胆苦苷生物合成途径研究进展[J].江苏农业科学,2014,42(03):4.
　Wang Caiyun,et al.Research progress of biosynthesis pathway of gentiopicroside[J].Jiangsu Agricultural Sciences,2014,42(22):4.
[10]慕金超,刘春芬.紫茄皮中花青素的提取研究[J].江苏农业科学,2014,42(04):227.
　Mu Jinchao,et al.Study on extraction of anthocyanins from peels of purple eggplants[J].Jiangsu Agricultural Sciences,2014,42(22):227.

备注/Memo

备注/Memo:
收稿日期：2021-12-26
基金项目：北京市农林科学院青年基金(编号：QNJJ202010)；北京市农林科学院科技创新能力建设专项(编号：KJCX20200601)。
作者简介：梁玉镯(1995—)，女，河北涿鹿人，硕士研究生，研究方向为菊花分子生物学。E-mail：1253368172@qq.com。
通信作者：黄丛林，博士，研究员，研究方向为花卉分子生物学及其生物技术育种。E-mail：conglinh@126.com。
更新日期/Last Update: 2022-11-20