[1]闫博巍,常传义,谷英楠,等.工业大麻CsWS/DGAT的生物信息学、亚细胞定位及表达模式分析[J].江苏农业科学,2026,54(1):35-44.
 Yan Bowei,et al.Bioinformatics,subcellular localization and expression analysis of CsWS/DGAT in industrial hemp[J].Jiangsu Agricultural Sciences,2026,54(1):35-44.
点击复制

工业大麻CsWS/DGAT的生物信息学、亚细胞定位及表达模式分析()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第54卷
期数:
2026年第1期
页码:
35-44
栏目:
生物技术
出版日期:
2026-01-05

文章信息/Info

Title:
Bioinformatics,subcellular localization and expression analysis of CsWS/DGAT in industrial hemp
作者:
闫博巍1常传义2谷英楠3隋月1郑楠1房郁妍1张明1张元野1张利国1
1.黑龙江省农业科学院经济作物研究所,黑龙江哈尔滨 150086; 2.哈尔滨市农业科学院,黑龙江哈尔滨 150029; 3.黑龙江省农业科学院农业遥感与信息研究所,黑龙江哈尔滨 150086
Author(s):
Yan Boweiet al
关键词:
工业大麻蜡酯合成/二酰基甘油酰基转移酶生物信息学冷胁迫表达模式
Keywords:
-
分类号:
S188;S563.301
DOI:
-
文献标志码:
A
摘要:
为明确工业大麻CsWS/DGAT基因的特性及表达特性,采用生物信息学手段对工业大麻WSD基因进行全基因组鉴定及特性分析,采用GFP融合表达载体瞬时转化烟草研究目的基因亚细胞定位,通过real-time qPCR检测方法研究目的基因的组织表达及冷胁迫下表达特异性模式。结果表明,工业大麻基因组中含有7个CsWSD基因家族成员,生物信息学分析结果表明,CsWSD家族成员包含大量顺式作用元件,包括逆境响应、光响应、植物激素响应及植物生长发育调控元件,暗示这些基因在环境适应等过程的潜在作用。亚细胞定位结果显示,CsWSD1.1定位于内质网上,CsWSD1.4定位于细胞质和细胞核。组织特异性表达分析结果表明,7个CsWSD基因在工业大麻不同器官和组织中差异表达。CsWSD基因受冷胁迫诱导差异表达,并在根和叶中的表达模式存在显著差异,这表明CsWSD基因可能在工业大麻冷响应过程发挥积极作用。
Abstract:
-

参考文献/References:

[1]Koch K,Ensikat H J. The hydrophobic coatings of plant surfaces:epicuticular wax crystals and their morphologies,crystallinity and molecular self-assembly[J]. Micron,2008,39(7):759-772.
[2]Kunst L,Samuels L. Plant cuticles shine:advances in wax biosynthesis and export[J]. Current Opinion in Plant Biology,2009,12(6):721-727.
[3]Kunst L,Samuels A L. Biosynthesis and secretion of plant cuticular wax[J]. Progress in Lipid Research,2003,42(1):51-80.
[4]Suh M C,Samuels A L,Jetter R,et al. Cuticular lipid composition,surface structure,and gene expression in Arabidopsis stem epidermis[J]. Plant Physiology,2005,139(4):1649-1665.
[5]Lü S Y,Song T,Kosma D K,et al. Arabidopsis cer8 encodes long-chain acyl-coa synthetase 1 (Lacs1) that has overlapping functions with LACS2 in plant wax and cutin synthesis[J]. The Plant Journal,2009,59(4):553-564.
[6]Bernard A,Domergue F,Pascal S,et al. Reconstitution of plant alkane biosynthesis in yeast demonstrates that Arabidopsis ECERIFERUM1 and ECERIFERUM3 are core components of a very-long-chain alkane synthesis complex[J]. The Plant Cell,2012,24(7):3106-3118.
[7]Greer S,Wen M,Bird D,et al. The cytochrome P450 enzyme CYP96A15 is the midchain alkane hydroxylase responsible for formation of secondary alcohols and ketones in stem cuticular wax of Arabidopsis[J]. Plant Physiology,2007,145(3):653-667.
[8]Rowland O,Zheng H Q,Hepworth S R,et al. CER4 encodes an alcohol-forming fatty acyl-coenzyme A reductase involved in cuticular wax production in Arabidopsis[J]. Plant Physiology,2006,142(3):866-877.
[9]Li F L,Wu X M,Lam P,et al. Identification of the wax ester synthase/acyl-coenzyme A:diacylglycerol acyltransferase WSD1 required for stem wax ester biosynthesis in Arabidopsis[J]. Plant Physiology,2008,148(1):97-107.
[10]Zhang N N,Mao Z J,Luo L,et al. Two bifunctional enzymes from the marine protist Thraustochytrium roseum:biochemical characterization of wax ester synthase/acyl-CoA:diacylglycerol acyltransferase activity catalyzing wax ester and triacylglycerol synthesis[J]. Biotechnology for Biofuels,2017,10:185.
[11]Kalscheuer R,Steinbüchel A. A novel bifunctional wax ester synthase/acyl-CoA:diacylglycerol acyltransferase mediates wax ester and triacylglycerol biosynthesis in Acinetobacter calcoaceticus ADP1[J]. Journal of Biological Chemistry,2003,278(10):8075-8082.
[12]King A,Nam J W,Han J X,et al. Cuticular wax biosynthesis in Petunia petals:cloning and characterization of an alcohol-acyltransferase that synthesizes wax-esters[J]. Planta,2007,226(2):381-394.
[13]Patwari P,Salewski V,Gutbrod K,et al. Surface wax esters contribute to drought tolerance in Arabidopsis[J]. The Plant Journal,2019,98(4):727-744.
[14]Cameron K D,Teece M A,Smart L B. Increased accumulation of cuticular wax and expression of lipid transfer protein in response to periodic drying events in leaves of tree tobacco[J]. Plant Physiology,2006,140(1):176-183.
[15]Kosma D K,Bourdenx B,Bernard A,et al. The impact of water deficiency on leaf cuticle lipids of Arabidopsis[J]. Plant Physiology,2009,151(4):1918-1929.
[16]Abdullah H M,Rodriguez J,Salacup J M,et al. Increased cuticle waxes by overexpression of WSD1 improves osmotic stress tolerance in Arabidopsis thaliana and Camelina sativa[J]. International Journal of Molecular Sciences,2021,22(10):5173.
[17]Seo P J,Lee S B,Suh M C,et al. The MYB96 transcription factor regulates cuticular wax biosynthesis under drought conditions in Arabidopsis[J]. The Plant Cell,2011,23(3):1138-1152.
[18]Lee S B,Suh M C. Cuticular wax biosynthesis is up-regulated by the MYB94 transcription factor in Arabidopsis[J]. Plant & Cell Physiology,2015,56(1):48-60.
[19]Lee S B,Kim H U,Suh M C. MYB94 and MYB96 additively activate cuticular wax biosynthesis in Arabidopsis[J]. Plant & Cell Physiology,2016,57(11):2300-2311.
[20]Burton R A,Andres M,Cole M,et al. Industrial hemp seed:from the field to value-added food ingredients[J]. Journal of Cannabis Research,2022,4(1):45.
[21]Zhang C,Yang J B,Meng W Q,et al. Genome-wide analysis of the WSD family in sunflower and functional identification of HaWSD9 involvement in wax ester biosynthesis and osmotic stress[J]. Frontiers in Plant Science,2022,13:975853.

相似文献/References:

[1]李家凤,汤开磊,刘思雨,等.外源脱落酸处理对盐胁迫下工业大麻苗期生长和生理的影响[J].江苏农业科学,2025,53(8):114.
 Li Jiafeng,et al.Effects of exogenous abscisic acid treatment on growth and physiology of industrial hemp seedlings under salt stress[J].Jiangsu Agricultural Sciences,2025,53(1):114.
[2]闫博巍,常传义,隋月,等.工业大麻CsDGAT1的生物信息学及表达模式分析[J].江苏农业科学,2025,53(19):16.
 Yan Bowei,et al.Bioinformatics and expression pattern analysis of CsDGAT1 gene in hemp[J].Jiangsu Agricultural Sciences,2025,53(1):16.

备注/Memo

备注/Memo:
收稿日期:2025-01-03
基金项目:黑龙江省农业科技创新跨越工程重点项目(编号:GX23GG04);黑龙江省自然科学基金(编号:LH2024C103);黑龙江省博士后研究人员落户黑龙江科研启动项目;国家自然科学基金(编号:32401703);中国博士后科学基金第14批特别资助项目(编号:2021T140187)。
作者简介:闫博巍(1990—),女,黑龙江哈尔滨人,博士,助理研究员,主要从事工业大麻逆境响应机理研究。E-mail:yanbowei21@outlook.com。
通信作者:张利国,博士,研究员,主要从事工业大麻育种及产业化应用研究。E-mail:zlg86@aliyun.com。
更新日期/Last Update: 2026-01-05