[1]陶佩琳,凤舞剑,曹丹,等.基于转录组和AlphaFold3的褪黑素抑制番茄灰霉病菌的靶标鉴定[J].江苏农业科学,2026,54(3):50-55.
　Tao Peilin,et al.Identification of melatonins inhibitory targets against Botrytis cinerea in tomato based on transcriptome and AlphaFold3[J].Jiangsu Agricultural Sciences,2026,54(3):50-55.
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基于转录组和AlphaFold3的褪黑素抑制番茄灰霉病菌的靶标鉴定()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第54卷
期数:
2026年第3期
页码:
50-55
栏目:
生物技术
出版日期:
2026-02-05

文章信息/Info

Title:
Identification of melatonins inhibitory targets against Botrytis cinerea in tomato based on transcriptome and AlphaFold3
作者:
陶佩琳; 凤舞剑; 曹丹; 张莹; 高峰
徐州生物工程职业技术学院/徐州市现代农业生物技术重点实验室，江苏徐州 221006
Author(s):
Tao Peilin; et al
关键词:
番茄灰霉病; 番茄; 褪黑素; 转录组; AlphaFold3; 靶标鉴定
Keywords:
-
分类号:
S436.412.1+3
DOI:
-
文献标志码:
A
摘要:
番茄灰霉病是一种严重危害栽培番茄的真菌病害，严重影响番茄的品质和产量。褪黑素作为一种天然的抑菌小分子，对病害防治具有巨大的潜力，但对番茄灰霉病的防治研究较少。为此，拟通过平板接菌和活体接种等方法测试褪黑素对番茄灰霉病菌的抑菌活性，并利用转录组对褪黑素影响的灰霉病菌中的靶标蛋白进行筛选，利用AlphaFold3和分子对接进行蛋白结构预测和结合特征分析。结果表明，褪黑素能有效抑制灰霉病菌的菌丝生长和致病力。通过进行转录组差异分析，共鉴定到840个上调差异表达基因和288个下调差异表达基因。GO功能富集结果表明，差异基因富集到的功能主要为谷氨酸合成酶活性、单氧酶活性、β-葡萄糖苷酶活性、氧化还原酶活性和过氧化物酶活性等，KEGG通路富集到脂质代谢、色氨酸代谢、谷胱甘肽代谢等通路。分子对接鉴定到，Bcin01g10380编码的NADPH还原酶与褪黑素的亲和性最高，存在28位精氨酸和279位天冬酰胺2个结合位点，结合能分别为-13.18 kJ/mol和-13.01 kJ/mol。本研究鉴定到的灰霉病菌核心靶标，有助于促进灰霉病菌绿色杀菌剂的开发和应用。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2025-06-07
基金项目：徐州市贾汪区设施蔬菜产业科技服务项目(编号：KC23139)。
作者简介：陶佩琳(1984—)，女，江苏南通人，博士，副教授，主要从事园艺植物生产、抗病种质创制等方面的研究。E-mail：xswtpl@126.com。
更新日期/Last Update: 2026-02-05