[1]张敏,周业飞,刘黑头.南京市规模鸡场产气荚膜梭菌流行病学调查及药敏试验[J].江苏农业科学,2026,54(3):216-222.
　Zhang Min,et al.Epidemiological investigation and drug susceptibility test of Clostridium perfringens in largescale chicken farms of Nanjing City[J].Jiangsu Agricultural Sciences,2026,54(3):216-222.
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南京市规模鸡场产气荚膜梭菌流行病学调查及药敏试验()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第54卷
期数:
2026年第3期
页码:
216-222
栏目:
动物科学与动物医学
出版日期:
2026-02-05

文章信息/Info

Title:
Epidemiological investigation and drug susceptibility test of Clostridium perfringens in largescale chicken farms of Nanjing City
作者:
张敏1; 周业飞2; 刘黑头3
1.江苏省南京市江宁区畜牧兽医与畜禽技术推广站，江苏南京 211100； 2.南京晓庄学院，江苏南京 211100； 3.南京禄口禽业发展有限公司，江苏南京 211100
Author(s):
Zhang Min; et al
关键词:
鸡场; 产气荚膜梭菌; 流行病学调查; 耐药性; 毒素基因
Keywords:
-
分类号:
S852.6
DOI:
-
文献标志码:
A
摘要:
调查南京市规模鸡场产气荚膜梭菌的流行特征、毒素基因分布及耐药性现状，明确其流行病学规律，为鸡坏死性肠炎的科学防控及抗生素合理使用提供依据。2023年12月至2024年9月，从南京市5个规模养鸡场采集150份泄殖腔拭子及75份环境样本。通过厌氧培养分离产气荚膜梭菌，对分离株进行生化鉴定及16S rRNA测序；采用PCR检测7种毒素基因(cpa、cpb、cpb2、etx、iap、cpe、netB)；通过K-B纸片扩散法测定菌株对11种抗生素的敏感性；利用多位点序列分型(MLST)分析分离株的亲缘关系。结果表明，共分离出产气荚膜梭菌31株，分离率为13.8%。其中，泄殖腔样本的分离率为18.0%，环境样本的分离率为5.3%；93.5%的分离株被鉴定为A型，6.5%为F型。cpb2基因的检出率为35.5%，所有分离株均未检测到cpb、etx、iap、netB基因；分离株对庆大霉素的耐药率最高，为100.0%，其次为链霉素(90.3%)；在最小生成树中，同一养殖场的产气荚膜梭菌分离株亲缘关系较近，聚为一支，不同养殖场的分离株之间亲缘关系较远。本研究系统揭示了南京市规模鸡场产气荚膜梭菌的流行规律与耐药特性，为针对性防控坏死性肠炎、优化养殖场抗生素使用策略提供了重要数据支撑，对保障养鸡业健康发展和食品安全具有实践价值。
Abstract:
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备注/Memo

备注/Memo:
收稿日期：2025-07-21
作者简介：张敏(1984—)，男，江苏南京人，高级兽医师，从事畜牧兽医工作。E-mail：286630979@qq.com。
更新日期/Last Update: 2026-02-05