[1]刘丽,余加龙,陈吉丽,等.秸秆类生物炭对猪粪堆肥腐熟质量、微生物群落及抗性基因的影响[J].江苏农业科学,2026,54(3):254-264.
　Liu Li,et al.Impacts of strawbased biochar on composting quality， microbial communities and resistance genes of pig manure[J].Jiangsu Agricultural Sciences,2026,54(3):254-264.
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秸秆类生物炭对猪粪堆肥腐熟质量、微生物群落及抗性基因的影响()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第54卷
期数:
2026年第3期
页码:
254-264
栏目:
农业资源与环境
出版日期:
2026-02-05

文章信息/Info

Title:
Impacts of strawbased biochar on composting quality， microbial communities and resistance genes of pig manure
作者:
刘丽1; 余加龙2; 陈吉丽1; 徐兴阳2; 蒋东2; 张海平2; 吕孟远2; 赵晓军2; 刘涛1
1.云南农业大学，云南昆明 650051； 2.云南省烟草公司昆明市公司，云南昆明 650051
Author(s):
Liu Li; et al
关键词:
秸秆类生物炭; 猪粪; ARG; MGE; 微生物群落; 堆肥
Keywords:
-
分类号:
X713；S141.4
DOI:
-
文献标志码:
A
摘要:
为掌握猪粪堆肥中添加秸秆类生物炭对堆肥腐熟质量及抗性基因的影响，选用玉米、水稻、小麦3种秸秆类生物炭与猪粪混匀开展了45 d的好氧堆肥试验，对照组不添加生物炭。通过分析堆肥后的理化性质、微生物群落、ARG、MGE间的差异，掌握秸秆类生物炭对猪粪堆肥质量及抗性基因的影响。结果显示，与CK相比，秸秆生物炭处理后高温期持续时间减少，pH值下降，有机物降解速度加快、电导率及C/N水平更符合高质量堆肥要求。秸秆生物炭的加入诱导了新的ARG产生，但有效降低了总ARG相对丰度。其中，对于利福霉素类、氨基糖苷类抗性基因的影响最大。此外，秸秆生物炭的加入还提升了细菌群落丰富度、多样性，显著改变了微生物的群落结构。环境因子与ARG相关性分析结果表明，ARG的丰度水平受到环境因子的显著影响，不同类型的ARG与环境因子的相关性不同。ARG、MGE与微生物群落的网络分析结果表明，ARG、MGE存在共同的潜在宿主微生物，且微生物群落和MGE是影响ARG变化的主要因素。值得注意的是，秸秆生物炭的加入降低了相关宿主微生物的丰度，从而有效抑制了MGE与ARG的水平基因转移。综上所述，秸秆生物炭的加入能有效提升猪粪堆肥腐熟质量及降低抗性基因的环境风险。本研究结果可为秸秆类生物炭在禽畜粪便堆肥中降低抗性基因环境风险提供理论参考。
Abstract:
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备注/Memo

备注/Memo:
收稿日期：2025-04-15
基金项目：云南省烟草公司昆明市公司科技计划(编号：KMYC202313)。
作者简介：刘丽(1999—)，女，云南大关人，硕士研究生，从事废弃物资源化利用研究。E-mail：359908571@qq.com。
通信作者：刘涛，博士，教授，从事药用植物品质形成研究，E-mail：gjbl818@163.com；赵晓军，硕士，农艺师，从事烟草栽培、烟叶分级研究，E-mail：626793262@qq.com。
更新日期/Last Update: 2026-02-05