[1]袁和奇,王瑜,栗帜,等.RNA干扰技术防治灰霉病的研究进展[J].江苏农业科学,2026,54(9):25-33.
　Yuan Heqi,et al.Research progress on control of gray mold disease based on RNA interference[J].Jiangsu Agricultural Sciences,2026,54(9):25-33.
点击复制

RNA干扰技术防治灰霉病的研究进展()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第54卷
期数:
2026年第9期
页码:
25-33
栏目:
专论与综述
出版日期:
2026-05-05

文章信息/Info

Title:
Research progress on control of gray mold disease based on RNA interference
作者:
袁和奇1; 王瑜1; 栗帜1; 乔岩1; 赵 娣1; 肖婷1; 段诗瑶1; 牛曼丽1; 张猛2
1.北京市农业农村科技发展中心，北京 101117； 2.河南农业大学植物保护学院，河南郑州 450002
Author(s):
Yuan Heqi; et al
关键词:
RNA干扰; 灰霉病; 研究进展
Keywords:
-
分类号:
S432.4+4
DOI:
-
文献标志码:
A
摘要:
由灰葡萄孢菌(Botrytis cinerea)引起的灰霉病，是一种全球性真菌病害，其危害范围十分广泛，造成的经济损失巨大。常用的灰霉病病害防治方法主要借助化学农药，容易产生非靶标生物毒害、农药残留、抗药性等问题。RNA干扰(RNA interference，RNAi)技术作为一种新兴的植物病害防治手段，因其高效、精准和环境友好等优势，逐渐成为灰霉病防控的研究热点。本文首先介绍灰霉病病原菌灰葡萄孢繁殖能力强、易发生病害流行的生物学特性，对其复杂多样的致病机制进行探讨；同时，依据双链RNA/小干扰 RNA(dsRNA/siRNA)的设计、双链RNA(dsRNA)的合成、dsRNA的转化、靶基因的沉默4个步骤，对RNA干扰的原理进行阐述；然后，着重从病毒诱导的基因沉默(VIGS)、宿主诱导的基因沉默(HIGS)、喷雾诱导的基因沉默(SIGS)和纳米结构递送的SIGS的4种不同RNA干扰方式，综述RNAi防治灰霉病的研究进展；最后，对RNA干扰技术的优势与风险挑战进行分析，并对RNA干扰技术控制灰霉病害的未来发展进行展望，以期为灰霉病的绿色高效防控提供理论依据。
Abstract:
-

参考文献/References:

［1］de Angelis G，Simonetti G，Chronopoulou L，et al. A novel approach to control Botrytis cinerea fungal infections：uptake and biological activity of antifungals encapsulated in nanoparticle based vectors［J］. Scientific Reports，2022，12：7989.
［2］Garfinkel A R. The history of Botrytis taxonomy，the rise of phylogenetics，and implications for species recognition［J］. Phytopathology，2021，111(3)：437-454.
［3］Ullah I，Yuan W B，Khalil H B，et al. Understanding Botrytis cinerea infection and gray mold management：a review paper on deciphering the roses thorn［J］. Phytopathology Research，2024，6(1)：42.
［4］Dean R，van Kan J A L，Pretorius Z A，et al. The Top 10 fungal pathogens in molecular plant pathology［J］. Molecular Plant Pathology，2012，13(7)：804.
［5］Fernández-Ortuo D，Grabke A，Li X P，et al. Independent emergence of resistance to seven chemical classes of fungicides in Botrytis cinerea［J］. Phytopathology，2015，105(4)：424-432.
［6］Rupp S，Weber R W S，Rieger D，et al. Spread of Botrytis cinerea strains with multiple fungicide resistance in German horticulture［J］. Frontiers in Microbiology，2017，7：2075.
［7］Zhang Q C，Zhang S，Wu B，et al. Methionine represses gray mold of tomato by keeping nitric oxide at an appropriate level via ethylene synthesis and signal transduction［J］. Food Chemistry，2024，461：140942.
［8］Wan N F，Fu L W，Dainese M，et al. Pesticides have negative effects on non-target organisms［J］. Nature Communications，2025，16：1360.
［9］Wytinck N，Manchur C L，Li V H，et al. dsRNA uptake in plant pests and pathogens：insights into RNAi-based insect and fungal control technology［J］. Plants，2020，9(12)：1780.
［10］Cagliari D，Dias N P，Galdeano D M，et al. Management of pest insects and plant diseases by non-transformative RNAi［J］. Frontiers in Plant Science，2019，10：1319.
［11］张中义. 中国真菌志.第二十六卷，葡萄孢属，柱隔孢属［M］. 北京：科学出版社，2006(2)：37-55.
［12］张莉，张博源，黄继红，等. 植物灰霉病生物防治研究进展［J］. 现代牧业，2021，5(2)：55-60.
［13］董金皋. 农业植物病理学［M］. 2版.北京：中国农业出版社，2007(8)：391-393.
［14］刘福平，黄台明，宋淑芳，等. 番茄灰霉病的生物学特性与防治研究进展［J］. 安徽农业科学，2014，42(31)：10924-10926.
［15］王方方，付清泉，史学伟，等. 灰霉病致病机理及其防治措施研究进展［J］. 中国果菜，2024，44(1)：47-53.
［16］马晨，周欣玥，王全. 番茄灰霉病生物防治的研究进展［J］. 园艺与种苗，2018，38(2)：61-62.
［17］陈宇春，陈敏，王其刚，等. 植物灰霉病及抗性研究进展［J］. 江苏农业科学，2020，48(15)：42-51，63.
［18］Staats M，van Kan J A L. Genome update of Botrytis cinerea strains B05.10 and T4［J］. Eukaryotic Cell，2012，11(11)：1413-1414.
［19］Bi K，Liang Y，Mengiste T，et al. Killing softly：a roadmap of Botrytis cinerea pathogenicity［J］. Trends in Plant Science，2023，28(2)：211-222.
［20］Fernández-Acero F J，Colby T，Harzen A，et al. 2-DE proteomic approach to the Botrytis cinerea secretome induced with different carbon sources and plant-based elicitors［J］. Proteomics，2010，10(12)：2270-2280.
［21］Manteau S，Abouna S，Lambert B，et al. Differential regulation by ambient pH of putative virulence factor secretion by the phytopathogenic fungus Botrytis cinerea［J］. FEMS Microbiology Ecology，2003，43(3)：359-366.
［22］Blanco-Ulate B，Labavitch J M，Vincenti E，et al. Hitting the wall：plant cell walls during Botrytis cinerea infections.In：Botrytis-the fungus，the pathogen and its management in agricultural systems［D］. Switzerland：Springer International Publishing，2016：361-386.
［23］Chakraborty N，Ghosh R，Ghosh S，et al. Reduction of oxalate levels in tomato fruit and consequent metabolic remodeling following overexpression of a fungal oxalate decarboxylase［J］. Plant Physiology，2013，162(1)：364-378.
［24］Prusky D，Lichter A. Activation of quiescent infections by postharvest pathogens during transition from the biotrophic to the necrotrophic stage［J］. FEMS Microbiology Letters，2007，268(1)：1-8.
［25］Siewers V，Smedsgaard J，Tudzynski P. The P450 monooxygenase BcABA1 is essential for abscisic acid biosynthesis in Botrytis cinerea［J］. Applied and Environmental Microbiology，2004，70(7)：3868-3876.
［26］Siewers V，Kokkelink L，Smedsgaard J，et al. Identification of an abscisic acid gene cluster in the grey mold Botrytis cinerea［J］. Applied and Environmental Microbiology，2006，72(7)：4619-4626.
［27］Jia H F，Chai Y M，Li C L，et al. Abscisic acid plays an important role in the regulation of strawberry fruit ripening［J］. Plant Physiology，2011，157(1)：188-199.
［28］Cantu D，Vicente A R，Greve L C，et al. The intersection between cell wall disassembly，ripening，and fruit susceptibility to Botrytis cinerea［J］. Proceedings of the National Academy of Sciences of the United States of America，2008，105(3)：859-864.
［29］Cantu D，Blanco-Ulate B，Yang L Y，et al. Ripening-regulated susceptibility of tomato fruit to Botrytis cinerea requires NOR but not RIN or ethylene［J］. Plant Physiology，2009，150(3)：1434-1449.
［30］Li H，Chen Y，Zhang Z Q，et al. Pathogenic mechanisms and control strategies of Botrytis cinerea causing post-harvest decay in fruits and vegetables［J］. Food Quality and Safety，2018，2(3)：111-119.
［31］Weiberg A，Wang M，Lin F M，et al. Fungal small RNAs suppress plant immunity by hijacking host RNA interference pathways［J］. Science，2013，342(6154)：118-123.
［32］Wang M，Weiberg A，Dellota E Jr，et al. Botrytis small RNA Bc-siR37 suppresses plant defense genes by cross-Kingdom RNAi［J］. RNA Biology，2017，14(4)：421-428.
［33］Choquer M，Fournier E，Kunz C，et al. Botrytis cinerea virulence factors：new insights into a necrotrophic and polyphageous pathogen［J］. FEMS Microbiology Letters，2007，277(1)：1-10.
［34］Veloso J，van Kan J A L. Many shades of grey in Botrytis-host plant interactions［J］. Trends in Plant Science，2018，23(7)：613-622.
［35］Fire A，Xu S Q，Montgomery M K，et al. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans［J］. Nature，1998，391(6669)：806-811.
［36］Islam M T，Sherif S M. RNAi-based biofungicides as a promising next-generation strategy for controlling devastating gray mold diseases［J］. International Journal of Molecular Sciences，2020，21(6)：2072.
［37］Mosquera S，Ginésy M，Bocos-Asenjo I T，et al. Spray-induced gene silencing to control plant pathogenic fungi：a step-by-step guide［J］. Journal of Integrative Plant Biology，2025，67(3)：801-825.
［38］龚流娥，应淑敏，张雅芬，等. 病虫害RNAi技术中的外源RNA递送策略［J］. 生物工程学报，2023，39(2)：459-471.
［39］李爽，闫更轩，田缘，等. RNA干扰技术防控作物真菌病害研究进展［J］. 北方园艺，2024(3)：127-136.
［40］Wilson R C，Doudna J A. Molecular mechanisms of RNA interference［J］. Annual Review of Biophysics，2013，42：217-239.
［41］Gong D，Ferrell J E Jr. Picking a winner：new mechanistic insights into the design of effective siRNAs［J］. Trends in Biotechnology，2004，22(9)：451-454.
［42］Patzel V，Rutz S，Dietrich I，et al. Design of siRNAs producing unstructured guide-RNAs results in improved RNA interference efficiency［J］. Nature Biotechnology，2005，23(11)：1440-1444.
［43］崔锦程，崔洁，卞小莹. 生产双链RNA工程菌的研究进展［J］. 生物工程学报，2025，41(2)：546-558.
［44］Elbashir S M，Harborth J，Lendeckel W，et al. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells［J］. Nature，2001，411(6836)：494-498.
［45］Donze O. RNA interference in mammalian cells using siRNAs synthesized with T7 RNA polymerase［J］. Nucleic Acids Research，2002，30(10)：e46.
［46］Voloudakis A E，Holeva M C，Sarin L P，et al. Efficient double-stranded RNA production methods for utilization in plant virus control［M］//Plant virology protocols.New York：Springer New York，2014：255-274.
［47］Niehl A，Soininen M，Poranen M M，et al. Synthetic biology approach for plant protection using dsRNA［J］. Plant Biotechnology Journal，2018，16(9)：1679-1687.
［48］Park M G，Kim W J，Choi J Y，et al. Development of a Bacillus thuringiensis based dsRNA production platform to control sacbrood virus in Apis cerana［J］. Pest Management Science，2020，76(5)：1699-1704.
［49］lvarez-Sánchez A R，Romo-Quinones C，Rosas-Quijano R，et al. Production of specific dsRNA against white spot syndrome virus in the yeast Yarrowia lipolytica［J］. Aquaculture Research，2018，49(1)：480-491.
［50］任恒泽，李丹莹，余亚婷，等. 植物VIGS载体构建策略研究与应用进展［J］. 园艺学报，2024，51(7)：1455-1473.
［51］Nowara D，Gay A，Lacomme C，et al. HIGS host-induced gene silencing in the obligate biotrophic fungal pathogen Blumeria graminis［J］. The Plant Cell，2010，22(9)：3130-3141.
［52］Ghag S B. Host induced gene silencing，an emerging science to engineer crop resistance against harmful plant pathogens［J］. Physiological and Molecular Plant Pathology，2017，100：242-254.
［53］冯丹丹，邓蕾，汪祖鹏，等. 寄主诱导的基因沉默在增强植物真菌病害抗性方面的研究进展［J］. 植物科学学报，2021，39(3)：316-323.
［54］Wang M，Jin H L. Spray-induced gene silencing：a powerful innovative strategy for crop protection［J］. Trends in Microbiology，2017，25(1)：4-6.
［55］Vetukuri R R，Dubey M，Kalyandurg P B，et al. Spray-induced gene silencing：an innovative strategy for plant trait improvement and disease control［J］. Crop Breeding and Applied Biotechnology，2021，21：e387921S11.DOI：10.1590/1984-70332021v21sa24.
［56］莫芹，蒋玮，陈一帆，等. 喷雾诱导基因沉默技术在植物真菌病害防治中的研究进展［J］. 中国生物防治学报，2022，38(5)：1316-1324.
［57］MacRae I J，Zhou K H，Li F，et al. Structural basis for double-stranded RNA processing by dicer［J］. Science，2006，311(5758)：195-198.
［58］Meister G，Tuschl T. Mechanisms of gene silencing by double-stranded RNA［J］. Nature，2004，431(7006)：343-349.
［59］Song J J，Smith S K，Hannon G J，et al. Crystal structure of argonaute and its implications for RISC slicer activity［J］. Science，2004，305(5689)：1434-1437.
［60］Schwarz D S，Hutvágner G，Du T T，et al. Asymmetry in the assembly of the RNAi enzyme complex［J］. Cell，2003，115(2)：199-208.
［61］Hannon G J. RNA interference［J］. Nature，2002，418(6894)：244-251.
［62］李志强，曹延婷，郭晓龙，等. 基因功能研究工具VIGS在植物中的应用［J］. 基因组学与应用生物学，2018，37(2)：915-923.
［63］Cao X Q，Yan H J，Liu X T，et al. A detached petal disc assay and virus-induced gene silencing facilitate the study of Botrytis cinerea resistance in rose flowers［J］. Horticulture Research，2019，6：136.
［64］Zhang H J，Yan M J，Deng R，et al. The silencing of DEK reduced disease resistance against Botrytis cinerea and Pseudomonas syringae pv. tomato DC3000 based on virus-induced gene silencing analysis in tomato［J］. Gene，2020，727：144245.
［65］Fu X D，Li J Z，Min D D，et al. LncRNA4504 involved in methyl jasmonate-induced resistance to Botrytis cinerea in postharvest tomato fruit［J］. Scientia Horticulturae，2022，305：111381.
［66］Li Z X，Yang S，Ma Y J，et al. Molecular mechanism of miR160d in regulating kiwifruit resistance to Botrytis cinerea［J］. Journal of Agricultural and Food Chemistry，2023，71(27)：10304-10313.
［67］Wang M，Weiberg A，Lin F M，et al. Bidirectional cross-kingdom RNAi and fungal uptake of external RNAs confer plant protection［J］. Nature Plants，2016，2(10)：16151.
［68］Xiong F J，Liu M，Zhuo F P，et al. Host-induced gene silencing of BcTOR in Botrytis cinerea enhances plant resistance to grey mould［J］. Molecular Plant Pathology，2019，20(12)：1722-1739.
［69］Capriotti L，Molesini B，Pandolfini T，et al. RNA interference-based strategies to control Botrytis cinerea infection in cultivated strawberry［J］. Plant Cell Reports，2024，43(8)：201.
［70］Xu Y，Tan J Y，Lu J X，et al. RAS signalling genes can be used as host-induced gene silencing targets to control fungal diseases caused by Sclerotinia sclerotiorum and Botrytis cinerea［J］. Plant Biotechnology Journal，2024，22(1)：262-277.
［71］Zotti M，dos Santos E A，Cagliari D，et al. RNA interference technology in crop protection against arthropod pests，pathogens and nematodes［J］. Pest Management Science，2018，74(6)：1239-1250.
［72］Andow D A，Zwahlen C. Assessing environmental risks of transgenic plants［J］. Ecology Letters，2006，9(2)：196-214.
［73］Yan S，Yu J，Han M，et al. Intercrops can mitigate pollen-mediated gene flow from transgenic cotton while simultaneously reducing pest densities［J］. Science of the Total Environment，2020，711：134855.
［74］Yan S，Zhu W L，Zhang B Y，et al. Pollen-mediated gene flow from transgenic cotton is constrained by physical isolation measures［J］. Scientific Reports，2018，8：2862.
［75］陈夕军，石童，陈宸，等. 喷施诱导的基因沉默(SIGS)技术控制植物病害研究进展［J］. 植物保护，2022，48(5)：15-22.
［76］Dubrovina A S，Kiselev K V. Exogenous RNAs for gene regulation and plant resistance［J］. International Journal of Molecular Sciences，2019，20(9)：2282.
［77］McLoughlin A G，Wytinck N，Walker P L，et al. Identification and application of exogenous dsRNA confers plant protection against Sclerotinia sclerotiorum and Botrytis cinerea［J］. Scientific Reports，2018，8：7320.
［78］Gu K X，Song X S，Xiao X M，et al. A β-tubulin dsRNA derived from Fusarium asiaticum confers plant resistance to multiple phytopathogens and reduces fungicide resistance［J］. Pesticide Biochemistry and Physiology，2019，153：36-46.
［79］Nerva L，Sandrini M，Gambino G，et al. Double-stranded RNAs (dsRNAs) as a sustainable tool against gray mold (Botrytis cinerea) in grapevine：effectiveness of different application methods in an open-air environment［J］. Biomolecules，2020，10(2)：200.
［80］Qiao L L，Lan C，Capriotti L，et al. Spray-induced gene silencing for disease control is dependent on the efficiency of pathogen RNA uptake［J］. Plant Biotechnology Journal，2021，19(9)：1756-1768.
［81］Duanis-Assaf D，Galsurker O，Davydov O，et al. Double-stranded RNA targeting fungal ergosterol biosynthesis pathway controls Botrytis cinerea and postharvest grey mould［J］. Plant Biotechnology Journal，2022，20(1)：226-237.
［82］Spada M，Pugliesi C，Fambrini M，et al. Silencing of the Slt2-Type map kinase bmp3 in Botrytis cinerea by application of exogenous dsRNA affects fungal growth and virulence on Lactuca sativa［J］. International Journal of Molecular Sciences，2021，22(10)：5362.
［83］Spada M，Pugliesi C，Fambrini M，et al. Knockdown of Bmp1 and Pls1 virulence genes by exogenous application of RNAi-inducing dsRNA in Botrytis cinerea［J］. International Journal of Molecular Sciences，2023，24(5)：4869.
［84］Patra J K，Das G，Fraceto L F，et al. Nano based drug delivery systems：recent developments and future prospects［J］. Journal of Nanobiotechnology，2018，16(1)：71.
［85］Yuan Y，Gu Z，Yao C，et al. Nucleic acid-based functional nanomaterials as advanced cancer therapeutics［J］. Small，2019，15(26)：1900172.
［86］付淑笙，江奕舟，蒋沁宏，等. 基于纳米递送系统的RNA农药及转基因植物研究进展［J］. 植物保护学报，2021，48(2)：267-274.
［87］Nio-Sánchez J，Sambasivam P T，Sawyer A，et al. BioClayTM prolongs RNA interference-mediated crop protection against Botrytis cinerea［J］. Journal of Integrative Plant Biology，2022，64(11)：2187-2198.
［88］Spada M，Pugliesi C，Fambrini M，et al. Spray-induced gene silencing (SIGS)：nanocarrier-mediated dsRNA delivery improves RNAi efficiency in the management of lettuce gray mold caused by Botrytis cinerea［J］. Agronomy，2025，15(1)：194.
［89］Shim G，Han S E，Yu Y H，et al. Trilysinoyl oleylamide-based cationic liposomes for systemic co-delivery of siRNA and an anticancer drug［J］. Journal of Controlled Release，2011，155(1)：60-66.
［90］Zhang Y T，Cui J，Zhou Y Z，et al. Liposome mediated double-stranded RNA delivery to silence ribosomal protein P0 in the tick Rhipicephalus haemaphysaloides［J］. Ticks and Tick-Borne Diseases，2018，9(3)：638-644.
［91］Islam M T，Davis Z，Chen L S，et al. Minicell-based fungal RNAi delivery for sustainable crop protection［J］. Microbial Biotechnology，2021，14(4)：1847-1856.
［92］Qiao L L，Nio-Sánchez J，Hamby R，et al. Artificial nanovesicles fords RNA delivery in spray-induced gene silencing for crop protection［J］. Plant Biotechnology Journal，2023，21(4)：854-865.
［93］Mudo L M D，Queiroz A F S，de Melo N F，et al. Stability evaluation of dsRNA and DNA encapsulated in chitosan nanoparticles［J］. BioNanoScience，2022，12(3)：774-784.
［94］Scarpin D，Nerva L，Chitarra W，et al. Characterisation and functionalisation of chitosan nanoparticles as carriers for double-stranded RNA (dsRNA) molecules towards sustainable crop protection［J］. Bioscience Reports，2023，43(11)：BSR20230817.
［95］Wu F L，Yan L，Zhao X Y，et al. Development of an RNA nanostructure for effective Botrytis cinerea control through spray-induced gene silencing without an extra nanocarrier［J］. Journal of Fungi，2024，10(7)：483.
［96］Mao Y B，Cai W J，Wang J W，et al. Silencing a cotton bollworm P450 monooxygenase gene by plant-mediated RNAi impairs larval tolerance of gossypol［J］. Nature Biotechnology，2007，25(11)：1307-1313.
［97］Gebremichael D E，Haile Z M，Negrini F，et al. RNA interference strategies for future management of plant pathogenic fungi：prospects and challenges［J］. Plants，2021，10(4)：650.
［98］张倩，郭玉鸽，曹可心，等. RNA干扰防控烟草黄瓜花叶病毒的效果［J］. 江苏农业科学，2024，52(13)：139-144.
［99］Bramlett M，Plaetinck G，Maienfisch P. RNA-based biocontrols：a new paradigm in crop protection［J］. Engineering，2020，6(5)：522-527.
［100］Lundgren J G，Duan J J. RNAi-based insecticidal crops：potential effects on nontarget species［J］. BioScience，2013，63(8)：657-665.
［101］Naito Y，Ui-Tei K. siRNA design software for a target gene-specific RNA interference［J］. Frontiers in Genetics，2012，3：102.
［102］路子琪，王静，张震，等. 基于RNAi的生物农药研究进展［J］. 浙江农业学报，2024，36(4)：968-977.
［103］Guan R B，Chu D D，Han X Y，et al. Advances in the development of microbial double-stranded RNA production systems for application of RNA interference in agricultural pest control［J］. Frontiers in Bioengineering and Biotechnology，2021，9：753790.
［104］Ahn S J，Donahue K，Koh Y，et al. Microbial-based double-stranded RNA production to develop cost-effective RNA interference application for insect pest management［J］. International Journal of Insect Science，2019，11(6)：1-8.
［105］何承帅，张辉，吴顺凡，等. 核酸农药纳米递送系统研究进展［J］. 农药学学报，2023，25(6)：1179-1197.
［106］Sang H，Kim J I. Advanced strategies to control plant pathogenic fungi by host-induced gene silencing (HIGS) and spray-induced gene silencing (SIGS)［J］. Plant Biotechnology Reports，2020，14(1)：1-8.
［107］Dubelman S，Fischer J，Zapata F，et al. Environmental fate of double-stranded RNA in agricultural soils［J］. PLoS One，2014，9(3)：e93155.
［108］Cooper A M，Silver K，Zhang J Z，et al. Molecular mechanisms influencing efficiency of RNA interference in insects［J］. Pest Management Science，2019，75(1)：18-28.
［109］Zhu K Y，Palli S R. Mechanisms，applications，and challenges of insect RNA interference［J］. Annual Review of Entomology，2020，65：293-311.
［110］Dalakouras A，Wassenegger M，Dadami E，et al. Genetically modified organism-free RNA interference：exogenous application of RNA molecules in plants［J］. Plant Physiology，2020，182(1)：38-50.
［111］Naegeli H，Birch A N，Casacuberta J，et al. Assessment of genetically modified maize MON 87411 for food and feed uses，import and processing，under Regulation (EC) No1829/2003 (application EFSA-GMO-NL-2015-124)［J］. EFSA Journal，2018，16(6)：e05310.
［112］Papadopoulou N，Devos Y，lvarez-Alfageme F，et al. Risk assessment considerations for genetically modified RNAi plants：EFSAs activities and perspective［J］. Frontiers in Plant Science，2020，11：445.
［113］关若冰，李海超，苗雪霞. RNA生物农药的商业化现状及存在问题［J］. 中国农业科学，2022，55(15)：2949-2960.
［114］Rodrigues T B，Mishra S K，Sridharan K，et al. First sprayable double-stranded RNA-based biopesticide product targets proteasome subunit beta type-5 in Colorado potato beetle (Leptinotarsa decemlineata)［J］. Frontiers in Plant Science，2021，12：728652.

相似文献/References:

[1]霍恒志,糜林,李金凤,等.草莓无害化栽培主要病害防治技术探讨[J].江苏农业科学,2013,41(04):115.
[2]朱丽梅,崔群香,蔡元琴,等.不同茄子品种田间病害调查及其抗病性鉴定[J].江苏农业科学,2013,41(06):96.
　Zhu Limei,et al.Field investigation of disease and disease resistance identification of different eggplant varieties[J].Jiangsu Agricultural Sciences,2013,41(9):96.
[3]张维,高朝宝,尹秀,等.棉蚜V-ATPase-A基因RNAi载体的构建及其在转基因拟南芥的基因表达[J].江苏农业科学,2013,41(10):17.
　Zhang Wei,et al.RNAi vector construction of V-ATPase-A gene from Aphis gossypii and its expression in transgenic Arabidopsis thaliana[J].Jiangsu Agricultural Sciences,2013,41(9):17.
[4]田连生,陈菲.木霉菌剂与多菌灵协同防治灰霉病试验[J].江苏农业科学,2013,41(12):132.
　Tian Liansheng,et al.Application experiment of Trichoderma mixed with carbendazim controlling gray mold[J].Jiangsu Agricultural Sciences,2013,41(9):132.
[5]王晓峨,刘恩玲.壳聚糖-铜的抑菌性能[J].江苏农业科学,2015,43(04):160.
　Wang Xiaoe,et al.Study on antifungal activity of chitosan-Cu[J].Jiangsu Agricultural Sciences,2015,43(9):160.
[6]牛贞福,徐金强,田召玲,等.诱抗剂对番茄植物学性状和灰霉病抗性的影响[J].江苏农业科学,2017,45(02):103.
　Niu Zhenfu,et al.Effects of inducing agents on botanical characters and gray mold resistance of tomato[J].Jiangsu Agricultural Sciences,2017,45(9):103.
[7]叶嘉,张倩茹,张浩.苦参生物碱对黄瓜幼苗灰霉病防治效果研究[J].江苏农业科学,2018,46(08):105.
　Ye Jia,et al.Controlling effect of matrine on Botrytis cinerea in Cucumis sativus seedling[J].Jiangsu Agricultural Sciences,2018,46(9):105.
[8]蒋妮,白丹宇,宋利沙,等.棘孢木霉F2菌株对三七灰霉病的生物防治作用[J].江苏农业科学,2018,46(20):94.
　Jiang Ni,et al.Biological control effect of Trichoderma asperellum F2 on Panax notoginseng grey mould[J].Jiangsu Agricultural Sciences,2018,46(9):94.
[9]贾薇,梁中午,张瑶瑶,等.兼抗白粉病和灰霉病草莓品种的筛选及田间抗性评价[J].江苏农业科学,2019,47(14):132.
　Jia Wei,et al.Screening of strawberry varieties resistant to powdery mildew and gray mold and evaluation of field resistance[J].Jiangsu Agricultural Sciences,2019,47(9):132.
[10]陈宇春,陈敏,王其刚,等.植物灰霉病及抗性研究进展[J].江苏农业科学,2020,48(15):42.
　Chen Yuchun,et al.Research progress of plant gray mold and resistance[J].Jiangsu Agricultural Sciences,2020,48(9):42.

备注/Memo

备注/Memo:
收稿日期：2025-06-19
基金项目：北京市农业农村科技发展中心综合保障项目(编号：11000025Y000003355746)；植物病虫害生物学国家重点实验室开放基金(编号：SKLOF202103)。
作者简介：袁和奇(1990—)，男，陕西安康人，硕士，农艺师，主要从事植物病虫害防控技术应用与推广工作。E-mail：yuanheqi15@163.com。
更新日期/Last Update: 2026-05-05