[1]苏冰冰,李佩璇,许珺,等.番茄SlRR基因家族的鉴定及表达与互作分析[J].江苏农业科学,2026,54(9):57-66.
 Su Bingbing,et al.Identification, expression patterns and interactions of SlRR gene family in tomato[J].Jiangsu Agricultural Sciences,2026,54(9):57-66.
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番茄SlRR基因家族的鉴定及表达与互作分析()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第54卷
期数:
2026年第9期
页码:
57-66
栏目:
生物技术
出版日期:
2026-05-05

文章信息/Info

Title:
Identification, expression patterns and interactions of SlRR gene family in tomato
作者:
苏冰冰李佩璇许珺丁静
南京农业大学园艺学院/作物遗传与种质创新利用全国重点实验室,江苏南京 210095
Author(s):
Su Bingbinget al
关键词:
番茄反应调节因子细胞分裂素基因表达激素互作
Keywords:
-
分类号:
S641.201
DOI:
-
文献标志码:
A
摘要:
反应调节因子(RR)蛋白是细胞分裂素信号转导途径中的关键组分,具有接收/响应上游激素信号,调控下游基因表达等功能,在番茄生长发育及细胞分裂素调控通路中发挥重要作用。基于番茄SL 4.0基因组及蛋白组数据,利用ScanProsite基序搜索和InternPro结构域分析等工具,鉴定得到33个番茄SlRR家族成员,并依据与拟南芥ARR/APRR的系统进化关系及蛋白结构域组成,将其分为A型、B型、C型、Clock型PRR、B型PRR 5类。其中,A型、B型SlRR分别包含7、10个成员;B型SlRR除RR结构域外,均含有1个Myb-type DNA结合域,很可能具有转录因子功能。多数SlRR基因位于番茄染色体两端5 Mb的相对高基因密度区。SlRR基因启动子中广泛富集光、激素、胁迫类响应元件,且全部A型和B型SlRR启动子中均含有B型RRs蛋白结合元件。多数A型SlRR的表达量在果实发育早期最高,随发育阶段的推进逐渐下降;而多数B型SlRR在果实发育中期或成熟中后期的表达量最高,显示了2类SlRR在番茄果实中的不同表达模式与功能。酵母单杂交试验表明,B型SlRR21与SlRR22可与生长素转运基因SlPIN3启动子直接结合。综上,本研究对番茄SlRR基因进行了鉴定、分类与特征分析,为揭示其在番茄生长发育及细胞分裂素与其他激素互作中的功能提供了参考。
Abstract:
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参考文献/References:

[1]Werner T,Motyka V,Laucou V,et al. Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity[J]. The Plant Cell,2003,15(11):2532-2550.
[2]Hwang I,Sheen J,Muller B. Cytokinin signaling networks[J]. Annual Review of Plant Biology,2012,63:353-380.
[3]To J P C,Haberer G,Ferreira F J,et al. Type-A Arabidopsis response regulators are partially redundant negative regulators of cytokinin signaling[J]. The Plant Cell,2004,16(3):658-671.
[4]Mason M G,Mathews D E,Argyros D A,et al. Multiple type-B response regulators mediate cytokinin signal transduction in Arabidopsis[J]. The Plant Cell,2005,17(11):3007-3018.
[5]Hosoda K,Imamura A,Katoh E,et al. Molecular structure of the GARP family of plant Myb-related DNA binding motifs of the Arabidopsis response regulators[J]. The Plant Cell,2002,14(9):2015-2029.
[6]Hwang I,Sheen J. Two-component circuitry in Arabidopsis cytokinin signal transduction[J]. Nature,2001,413(6854):383-389.
[7]Mason M G,Li J,Mathews D E,et al. Type-B response regulators display overlapping expression patterns in Arabidopsis[J]. Plant Physiology,2004,135(2):927-937.
[8]Ishida K,Yamashino T,Yokoyama A,et al. Three type-B response regulators,ARR1,ARR10 and ARR12,play essential but redundant roles in cytokinin signal transduction throughout the life cycle of Arabidopsis thaliana[J]. Plant & Cell Physiology,2008,49(1):47-57.
[9]Zubo Y O,Blakley I C,Yamburenko M V,et al. Cytokinin induces genome-wide binding of the type-B response regulator ARR10 to regulate growth and development in Arabidopsis[J]. Proceedings of the National Academy of Sciences of the United States of America,2017,114(29):E5995-E6004.
[10]Xie M,Chen H,Huang L,et al. A B-ARR-mediated cytokinin transcriptional network directs hormone cross-regulation and shoot development[J]. Nature Communications,2018,9(1):1604.
[11]Tanksley S D. The genetic,developmental,and molecular bases of fruit size and shape variation in tomato[J]. The Plant Cell,2004,16(Suppl):S181-S189.
[12]The Tomato Genome Consortium. The tomato genome sequence provides insights into fleshy fruit evolution[J]. Nature,2012,485(7400):635-641.
[13]He Y,Liu X,Ye L,et al. Genome-wide identification and expression analysis of two-component system genes in tomato[J]. International Journal of Molecular Sciences,2016,17(8):1204.
[14]Wang J,Xia J,Song Q,et al. Genome-wide identification,genomic organization and expression profiles of SlARR-B gene family in tomato[J]. Journal of Applied Genetics,2020,61(3):391-404.
[15]Bae Y,Song S J,Lim C W,et al. Tomato salt-responsive pseudo-response regulator 1,SlSRP1,negatively regulates the high-salt and dehydration stress responses[J]. Physiologia Plantarum,2023,175(6):e14082.
[16]Irum S,Rehman N,Inam S,et al. Genome-wide identification and expression profiling of Pseudo-Response Regulator (PRR) gene family in tomato[J]. Environmental and Experimental Botany,2024,220:105683.
[17]Chen C,Wu Y,Li J,et al. TBtools-Ⅱ:a “one for all,all for one” bioinformatics platform for biological big-data mining[J]. Molecular Plant,2023,16(11):1733-1742.
[18]苏冰冰.番茄中细胞分裂素信号通路关键转录因子的鉴定与CRISPR/Cas突变体构建[D]. 南京:南京农业大学.2025:15-16.
[19]de Castro E,Sigrist C J,Gattiker A,et al. ScanProsite:detection of PROSITE signature matches and ProRule-associated functional and structural residues in proteins[J]. Nucleic Acids Research,2006,34:W362-W365.
[20]Jones P,Binns D,Chang H Y,et al. InterProScan 5:genome-scale protein function classification[J]. Bioinformatics,2014,30(9):1236-1240.
[21]Gruhn N,Halawa M,Snel B,et al. A subfamily of putative cytokinin receptors is revealed by an analysis of the evolution of the two-component signaling system of plants[J]. Plant Physiology,2014,165(1):227-237.
[22]Gan L,Song M,Wang X,et al. Cytokinins is involved in regulation of tomato pericarp thickness and fruit size[J]. Horticulture Research,2022,9:uhab041.
[23]Liu H,Chen R,Li H,et al. Genome-wide identification and expression analysis of SlRR genes in response to abiotic stress in tomato[J]. Plant Biology,2023,25(2):322-333.
[24]Zubo Y O,Schaller G E. Role of the Cytokinin-activated type-b response regulators in hormone crosstalk[J]. Plants,2020,9(2):166.

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备注/Memo

备注/Memo:
收稿日期:2025-05-27
基金项目:大学生创新创业训练计划(编号:S202510307024);江苏高校优势学科建设工程。
作者简介:苏冰冰(1995—),女,河南商丘人,硕士研究生,研究方向为蔬菜生理与分子生物学。E-mail:subing1234562022@163.com。
通信作者:丁静,博士,副教授,研究方向为园艺作物生物技术与育种。E-mail:jding@njau.edu.cn。
更新日期/Last Update: 2026-05-05