[1]李辉,丁顺欣,沈婕,等.猪IDO1基因过表达细胞模型的构建[J].江苏农业科学,2026,54(9):201-208.
 Li Hui,et al.Construction of a cell model with overexpressed porcine IDO1 gene[J].Jiangsu Agricultural Sciences,2026,54(9):201-208.
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IDO1基因过表达细胞模型的构建()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第54卷
期数:
2026年第9期
页码:
201-208
栏目:
动物科学与动物医学
出版日期:
2026-05-05

文章信息/Info

Title:
Construction of a cell model with overexpressed porcine IDO1 gene
作者:
李辉123丁顺欣123沈婕124邓彦飞4
1.江苏省农业科学院畜牧研究所/江苏省农业种质资源保护与利用平台,江苏南京 210014; 2.江苏省畜禽精准育种工程研究中心/农业农村部种养结合重点实验室,江苏南京 210014; 3.南京农业大学动物科学技术学院,江苏南京 210095; 4.广西大学动物科学技术学院,广西南宁 530005
Author(s):
Li Huiet al
关键词:
色氨酸犬尿氨酸IDO1过表达293T
Keywords:
-
分类号:
S828.3
DOI:
-
文献标志码:
A
摘要:
为深入了解猪颗粒细胞中色氨酸代谢机制及其代谢产物犬尿氨酸对细胞功能的影响,构建一种猪IDO1基因过表达的细胞模型。根据GenBank收录的猪IDO1基因序列(NM_001246240.1),舍去其编码区终止密码子后进行人工合成并插入真核表达载体pcDNA3.1-EGFP的NheⅠ和AgeⅠ酶切位点之间,将构建的重组表达载体命名为pcDNA3.1-IDO1-EGFP。提取无内毒素重组表达载体并转染293T细胞,继续培养24 h后收集细胞和培养液上清,分别利用Western blot和HPLC-MS/MS技术分别检测细胞中猪IDO1基因的表达情况及培养液中色氨酸和犬尿氨酸含量的变化。结果表明,pcDNA3.1-IDO1-EGFP重组表达载体转染293T细胞成功表达了猪IDO1蛋白且与EGFP形成融合蛋白。相较于对照组,猪IDO1基因过表达的细胞培养液中色氨酸含量显著降低,而犬尿氨酸含量显著升高。结果表明,过表达猪IDO1基因的细胞模型构建成功,为进一步探究色氨酸在细胞中的代谢机制及犬尿氨酸对细胞功能的影响提供了便利。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期:2025-07-03
基金项目:江苏省农业科技自主创新资金(编号:CX[24]3087);国家自然科学基金(编号:32272883);国家生猪产业技术体系建设专项(编号:CARS-PIG-35);江苏省种业振兴揭榜挂帅项目(编号:JBGS[2021]100)。
通信作者:李辉 (1982—),男,山东惠民人,博士,研究员,从事动物繁殖调控研究。E-mail:lhlydk@126.com。
更新日期/Last Update: 2026-05-05