[1]伍淑娟,王光安.黄芪SBP基因家族鉴定及其对高温和干旱胁迫的响应[J].江苏农业科学,2026,54(10):47-60.
　Wu Shujuan,et al.Identification of SBP gene family in Astragalus membranaceus and their responses to heat and drought stress[J].Jiangsu Agricultural Sciences,2026,54(10):47-60.
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黄芪SBP基因家族鉴定及其对高温和干旱胁迫的响应()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第54卷
期数:
2026年第10期
页码:
47-60
栏目:
耐温度胁迫基因鉴定
出版日期:
2026-05-20

文章信息/Info

Title:
Identification of SBP gene family in Astragalus membranaceus and their responses to heat and drought stress
作者:
伍淑娟1; 王光安2
1.濮阳医学高等专科学校，河南濮阳457000； 2.河南中医药大学，河南郑州 45008
Author(s):
Wu Shujuan; et al
关键词:
黄芪; SBP基因家族; 高温胁迫; 干旱胁迫; 亚细胞定位
Keywords:
-
分类号:
S188
DOI:
-
文献标志码:
A
摘要:
SQUAMOSA启动子结合蛋白(SBP)转录因子家族是植物特有的一类转录调控因子，在植物的发育及非生物胁迫响应中发挥着重要作用。为深入解析黄芪(Astragalus membranaceus)SBP基因家族的结构特征及其在高温和干旱胁迫下的响应机制，在黄芪全基因组范围内共鉴定出17个AmSBP基因(AmSBP1~AmSBP17)。这些蛋白整体表现为亲水性，但预测为不稳定蛋白，主要定位于细胞核内。系统进化分析显示，AmSBP基因可划分为多个亚家族，且同一亚群内的蛋白结构及基因结构具有高度一致性。染色体定位结果表明，AmSBP基因在染色体上分布不均，主要富集于第1号和第2号染色体，而第8号染色体上未检测到相关基因。基因重复分析发现，黄芪中存在4对片段重复基因对，提示该基因家族的扩张主要通过片段复制实现。种间共线性分析进一步揭示，AmSBP基因与拟南芥存在较强的同源性，说明其在系统发育上与双子叶植物关系更为密切。对启动子区域的顺式作用元件分析发现，AmSBP基因启动子中富含与光照、激素、干旱及低温响应相关的调控元件。qRT-PCR表达分析显示，AmSBP4基因在高温胁迫(40 ℃)下显著上调，其表达量较对照提高约30倍；在干旱胁迫(15% PEG)1 h和12 h时分别升高至40倍和60倍，表明其为响应高温干旱胁迫的关键候选基因。进一步的亚细胞定位试验确认AmSBP4定位于细胞核内。本研究系统解析了黄芪AmSBP基因家族的结构、进化关系及其对高温与干旱胁迫的响应模式，明确了AmSBP4基因作为潜在关键功能基因的作用，可为深入揭示黄芪抗逆分子机制及遗传改良提供理论依据和基因资源。
Abstract:
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备注/Memo

备注/Memo:
收稿日期：2025-08-09
基金项目：河南中医药大学特色骨干学科顶目(编号：STG-ZYX02-202114)。
作者简介：伍淑娟(1979—)，女，河南濮阳人，讲师，主要从事中医药研究。E-mail：wsj9791000@163.com。
通信作者：王光安，博士，副教授，从事中医学研究。E-mail：wgz1979001126@126.com。
更新日期/Last Update: 2026-05-20