[1]莫双凤,张蕊,刘蝶,等.枳椇属植物的叶绿体基因组特征、密码子偏好性及系统发育分析[J].江苏农业科学,2026,54(13):80-89.
Mo Shuangfeng,et al.Chloroplast genome characteristics,codon preference,and phylogenetic analysis of Hovenia plants[J].Jiangsu Agricultural Sciences,2026,54(13):80-89.
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枳椇属植物的叶绿体基因组特征、密码子偏好性及系统发育分析(
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]
- 卷:
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第54卷
- 期数:
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2026年第13期
- 页码:
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80-89
- 栏目:
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生物技术
- 出版日期:
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2026-07-05
文章信息/Info
- Title:
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Chloroplast genome characteristics,codon preference,and phylogenetic analysis of Hovenia plants
- 作者:
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莫双凤; 张蕊; 刘蝶; 谢吉兆; 杨婼涵; 张党权; 丁申; 李明婉
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河南农业大学林学院,河南郑州 450046
- Author(s):
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Mo Shuangfeng; et al
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- 关键词:
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枳椇属; 俅江枳椇; 叶绿体基因组; 基因组特征; 系统发育
- Keywords:
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- 分类号:
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S188;S718.43
- DOI:
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- 文献标志码:
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A
- 摘要:
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枳椇属隶属于鼠李科,因其弯曲膨大、肉质肥厚的果序轴形态而闻名。关于枳椇属植物遗传方面的探索,特别是在叶绿体基因组的结构与多样性方面较为有限。鉴于叶绿体基因组在系统发育研究中的重要性,深入了解枳椇属叶绿体基因组的结构和特征十分必要。对枳椇属2个物种[北枳椇(Hovenia dulcis)和毛果枳椇(H.trichocarpa)]及1个变种[俅江枳椇(H.acerba var.kiukiangensis)]的叶绿体基因组进行高通量测序、组装和注释,联合已公布的2个枳椇(H.acerba)的叶绿体基因组序列,比较分析枳椇属植物叶绿体基因组的结构特征,构建本属物种间的系统发育关系。结果发现,具有典型四分体结构的枳椇属叶绿体基因组序列长度在161 628~161 853 bp之间,GC含量36.60%~36.70%。完整基因组共包含133~134个基因,除去19~20个重复基因后,包含80个PCG基因、30个tRNA基因和4个rRNA基因。密码子偏好性分析显示,偏好使用A/U结尾的密码子;散在重复序列检测结果显示主要为正向重复和回文重复;简单重复序列分析发现,SSR以单碱基重复为主,主要组成碱基为A/T;构建的鼠李科植物的系统发育关系显示,枳椇属物种聚为独立的单系类群,与枣属(Ziziphus)、马甲子属(Paliurus)植物亲缘关系最近;属内俅江枳椇与枳椇以100%的支持率紧密地聚在一起,与系统树基部的毛果枳椇、北枳椇关系相对稍远。
- Abstract:
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参考文献/References:
[1]张玉超,朱思洁,刘良禹,等. 枳椇果梗多糖对小鼠急性酒精性肝损伤的改善效应研究[J]. 食品与发酵工业,2025,51(7):185-193.
[2]卢孟飞,陈瞳晖,莫双凤,等. 拐枣主栽品种果实的经济性状和营养成分分析及综合评价[J]. 经济林研究,2024,42(2):112-121.
[3]王洁. 提取方式及复合纳米硒对拐枣多糖结构及活性的影响[D]. 北京:中国农业科学院,2023.
[4]中国科学院中国植物志编辑委员会. 中国植物志[M]. 北京:科学出版社,1982.
[5]Hu H H,Cheng W C. Some new trees from Yunnan[J]. Bull Fan Mem Inst of Biol,New Ser,1948,1(2):191-198.
[6]Karimov B,Tojibaev S K,Azimova D,et al. Comparative analysis of complete chloroplast genomes of Cousinia(Asteraceae) species[J]. Frontiers in Plant Science,2025,16:1522950.
[7]刘起麟,李金泽,丁佳渝,等. 蓼属叶绿体基因组密码子偏好性分析及比较特征[J]. 中国农业科技导报,2025,27(4):87-98.
[8]Graham M R,Kaur N,Jones C S,et al. A phoenix in the greenhouse:characterization and phylogenomics of complete chloroplast genomes sheds light on the putatively extinct-in-the-wild Solanum ensifolium(Solanaceae)[J]. BMC plant biology,2025,25(1):320-320.
[9]Li X P,Xu H,Yu J Y,et al. Comparative and phylogenetic analysis of Potentilla and Dasiphora (Rosaceae) based on plastid genome[J]. BMC Plant Biology,2025,25(1):176.
[10]Li M W,Ye X F,Bi H T. Characterization of the complete chloroplast genome of two Hovenia species (Rhamnaceae)[J]. Mitochondrial DNA Part B,2020,5(2):1731-1732.
[11]Yin Y L,Tao J H,Yang X M,et al. The first complete chloroplast genome of Hovenia acerba Lindl.[J]. Mitochondrial DNA Part B,2020,5(1):658-659.
[12]Ling L Z,Zhang S D. The complete chloroplast genome of Hovenia dulcis(Rhamnaceae)[J]. Mitochondrial DNA Part B,2020,5(1):665-666.
[13]Zhang L,Mao R L,Bi H T,et al. Characterization of the complete chloroplast genome of Hovenia acerba(Rhamnaceae)[J]. Mitochondrial DNA Part B,2020,5(1):934-935.
[14]Liu D,Tong B Q,Li W Q,et al. The first complete chloroplast genome of Hovenia dulcis Thunb.(Rhamnaceae)[J]. Mitochondrial DNA Part B,2021,6(3):916-917.
[15]Dierckxsens N,Mardulyn P,Smits G. NOVOPlasty:de novo assembly of organelle genomes from whole genome data[J]. Nucleic Acids Research,2017,45(4):e18.
[16]Wick R R,Schultz M B,Zobel J,et al. Bandage:interactive visualization of de novo genome assemblies[J]. Bioinformatics,2015,31(20):3350-3352.
[17]Shi L C,Chen H M,Jiang M,et al. CPGAVAS2,an integrated plastome sequence annotator and analyzer[J]. Nucleic Acids Research,2019,47(W1):W65-W73.
[18]Greiner S,Lehwark P,Bock R. OrganellarGenomeDRAW (OGDRAW) version 1.3.1:expanded toolkit for the graphical visualization of organellar genomes[J]. Nucleic Acids Research,2019,47(W1):W59-W64.
[19]López J L,Lozano M J,Lagares A Jr,et al. Codon usage heterogeneity in the multipartite prokaryote genome:selection-based coding bias associated with gene location,expression level,and ancestry[J]. mBio,2019,10(3):e00505-e00519.
[20]Kurtz S,Choudhuri J V,Ohlebusch E,et al. REPuter:the manifold applications of repeat analysis on a genomic scale[J]. Nucleic Acids Research,2001,29(22):4633-4642.
[21]Beier S,Thiel T,Münch T,et al. MISA-web:a web server for microsatellite prediction[J]. Bioinformatics,2017,33(16):2583-2585.
[22]Liu C,Shi L C,Zhu Y J,et al. CpGAVAS,an integrated web server for the annotation,visualization,analysis,and GenBank submission of completely sequenced chloroplast genome sequences[J]. BMC Genomics,2012,13:715.
[23]Frazer K A,Pachter L,Poliakov A,et al. VISTA:computational tools for comparative genomics[J]. Nucleic Acids Research,2004,32(Web Server issue):W273-W279.
[24]Mayor C,Brudno M,Schwartz J R,et al. VISTA:visualizing global DNA sequence alignments of arbitrary length[J]. Bioinformatics,2000,16(11):1046-1047.
[25]Rozewicki J,Li S L,Amada K M,et al. MAFFT-DASH:integrated protein sequence and structural alignment[J]. Nucleic Acids Research,2019,47(W1):W5-W10.
[26]Kalyaanamoorthy S,Minh B Q,Wong T K F,et al. ModelFinder:fast model selection for accurate phylogenetic estimates[J]. Nature Methods,2017;14(6):587-589.
[27]Nguyen L T,Schmidt H A,von Haeseler A,et al. IQ-TREE:a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies[J]. Molecular Biology and Evolution,2015,32(1):268-274.
[28]Freccero J. The fig tree and the laurel:petrarchs poetics[J]. Diacritics,1975,5(1):34-40.
[29]Jansen R K,Raubeson L A,Boore J L,et al. Methods for obtaining and analyzing whole chloroplast genome sequences[J]. Methods in Enzymology,2005,395:348-384.
[30]吴宪明,吴松锋,任大明,等. 密码子偏性的分析方法及相关研究进展[J]. 遗传,2007,29(4):420-426.
[31]胡晓聪,刘秀云,胥明,等. 白灵山红山茶叶绿体基因组特征分析[J]. 西北林学院学报,2024,39(6):34-44.
[32]Darling A C E,Mau B,Blattner F R,et al. Mauve:multiple alignment of conserved genomic sequence with rearrangements[J]. Genome Research,2004,14(7):1394-403.
[33]刘旭东,张玉超,朱思洁,等. 枳椇果梗多糖的提取工艺优化及其抗氧化性[J]. 食品工业科技,2023,44(11):230-237.
[34]Wanichthanarak K,Nookaew I,Pasookhush P,et al. Revisiting chloroplast genomic landscape and annotation towards comparative chloroplast genomes of Rhamnaceae[J]. BMC Plant Biology,2023,23(1):59-59.
[35]Ma Q Y,Li S X,Bi C W,et al. Complete chloroplast genome sequence of a major economic species,Ziziphus jujuba(Rhamnaceae)[J]. Current Genetics,2017,63(1):117-129.
[36]Shi W B,Hu S Q,Song W C,et al. Uncovering the first complete chloroplast genomics,comparative analysis,and phylogenetic relationships of the medicinal plants Rhamnus cathartica and Frangula alnus (Rhamnaceae)[J]. Physiology and Molecular Biology of Plants,2023,29(6):855-869.
[37]Clowes C,Fowler R M,Fahey P S,et al. Big trees of small baskets:phylogeny of the Australian genus Spyridium(Rhamnaceae:Pomaderreae),focusing on biogeographic patterns and species circumscriptions[J]. Australian Systematic Botany,2022,35(2):95-119.
[38]Wang Y H,Chen S Y,Zhang S D. Characterization of the complete chloroplast genome of Berchemiella wilsonii var.wilsonii(Rhamnaceae),an endangered species endemic to China[J]. Conservation Genetics Resources,2018,10(1):39-41.
[39]孟祎,李菁,杜少兵,等. 17种鼠李科植物的叶绿体基因组特征和密码子偏好性分析[J]. 分子植物育种,2023:1-19(2023-08-22)[2025-06-26]. https://link.cnki.net/urlid/46.1068.S.20230822.1011.002.
[40]Luciński R,Jackowski G. The structure,functions and degradation of pigment-binding proteins of photosystem Ⅱ[J]. Acta biochimica Polonica,2006,53(4):693-708.
[41]孔卫青,禚苏,杨金宏. 苋色藜叶绿体基因组的解析与系统发育分析[J]. 草地学报,2025,33(3):739-747.
[42]张书东,凌立贞,谢丹丹,等. 白花重楼叶绿体基因组特征及系统发育分析[J]. 热带作物学报,2023,44(8):1551-1560.
[43]李薇,王倩,胡晓艳,等. 银杏叶绿体基因组密码子使用偏性分析[J]. 林业调查规划,2021,46(6):28-34.
[44]杨晓婷. 枣叶绿体基因组SSR开发及遗传多样性研究[D]. 杨凌:西北农林科技大学,2014.
[45]段义忠,张凯. 沙冬青属植物叶绿体基因组对比和系统发育分析[J]. 西北植物学报,2020,40(8):1323-1332.
[46]Richardson J E,Chatrou L W,Mols J B,et al. Historical biogeography of two cosmopolitan families of flowering plants:Annonaceae and Rhamnaceae[J]. Philosophical transactions of the Royal Society of London Series B:Biological Sciences,2004,359(1450):1495-508.
[47]Jud N A,Gandolfo M A,Iglesias A,et al. Flowering after disaster:Early Danian buckthorn (Rhamnaceae) flowers and leaves from Patagonia[J]. PLoS one,2017,12(5):e0176164.
[48]Shi W B,Huang Y H,Hu S Q,et al. Exploring the chloroplast genomics,comparative analysis,evolution,and phylogenetic relationships of Phylica pubescens(Rhamnaceae) in the Cape Flora[J]. South African Journal of Botany,2024,164:374-385.
备注/Memo
- 备注/Memo:
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收稿日期:2025-07-14
基金项目:国家自然科学基金青年科学基金(编号:32000267);河南省科技攻关项目(编号:232102111018)。
作者简介:莫双凤(2000—),女,河南信阳人,硕士研究生,主要从事植物系统进化与分类研究,E-mail:msf0420@163.com;共同第一作者:张蕊(2001—),女,河南南阳人,硕士研究生,主要从事植物系统进化与分类研究,E-mail:1521622517@qq.com。
通信作者:李明婉,博士,副教授,主要从事植物系统进化与分类研究,E-mail:limingwan3@126.com;丁申,博士,讲师,主要从事林木抗逆分子生理研究,E-mail:dingshen040033@163.com。
更新日期/Last Update:
2026-07-05