[1]侯孟昕,莫正海,左继林,等.薄壳山核桃间作不同作物对土壤养分、酶活性及微生物多样性的影响[J].江苏农业科学,2026,54(13):276-283.
 Hou Mengxin,et al.Effects of intercropping different crops with thinshelled pecans on soil nutrient,enzyme activity and microbial diversity[J].Jiangsu Agricultural Sciences,2026,54(13):276-283.
点击复制

薄壳山核桃间作不同作物对土壤养分、酶活性及微生物多样性的影响()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第54卷
期数:
2026年第13期
页码:
276-283
栏目:
农业资源与环境
出版日期:
2026-07-05

文章信息/Info

Title:
Effects of intercropping different crops with thinshelled pecans on soil nutrient,enzyme activity and microbial diversity
作者:
侯孟昕1莫正海1左继林2胡龙娇1王国明1宣继萍1翟敏1
1.江苏省中国科学院植物研究所,江苏南京 210014; 2.江西省林业科学院,江西南昌 330013
Author(s):
Hou Mengxinet al
关键词:
薄壳山核桃间作高通量测序土壤养分微生物多样性
Keywords:
-
分类号:
S344.2
DOI:
-
文献标志码:
A
摘要:
分析薄壳山核桃果园间种对土壤环境的影响,以期为生产提供指导。设置3种间种处理,分别为薄壳山核桃/红薯(Ipomoea batatas,IB)、薄壳山核桃/芝麻(Sesamum indicum,SI)及薄壳山核桃/玉米(Zea mays,ZM),以薄壳山核桃单作为对照,探究不同间作处理下土壤养分含量、酶活性及微生物多样性的变化规律。结果表明,3种间作处理下土壤pH值均存在一定程度的降低,土壤有机质含量仅在ZM处理下无显著变化,其余间作处理均发生显著降低。总体而言,间种后并未引起土壤全氮、硝态氮含量的降低,但引起全磷、速效磷、全钾、速效钾含量降低。纤维素酶的活性在3种间作处理下均没有显著变化,过氧化氢酶活性在ZM处理中下降,其他处理没有变化,脲酶活性在3种处理下均下降,而酸性磷酸酶、蔗糖酶的活性在不同处理下既有上升也有下降。高通量测序共鉴定出19 904个细菌和 1 417 个真菌扩增子序列变异。总体上,门分类水平下的优势细菌和真菌相对丰度有所提高。α、β多样性分析表明,间作能在一定程度上增加微生物的种类并引起群体结构的变化。组间差异分析分别鉴定出93、17个相对丰度与对照有显著差异的细菌、真菌。薄壳山核桃林下间种红薯、芝麻、玉米会导致一定程度的营养损失,但整体上提高了土壤微生物的多样性,建议在间作这3种农作物时增施磷肥和钾肥。
Abstract:
-

参考文献/References:

[1]殷国兰,朱曼,陈宇,等. 薄壳山核桃在川南地区的生长特性[J]. 四川林业科技,2021,42(6):102-105.
[2]张日清,吕芳德,张勖,等. 美国山核桃在我国扩大引种的可行性分析[J]. 经济林研究,2005,23(4):1-10.
[3]张日清,吕芳德,何方. 美国山核桃引种栽培区划研究Ⅰ原生境与新生境自然条件比较[J]. 中南林学院学报,2001,21(2):1-5.
[4]杜雅琦,赵荣,赵铁蕊.我国木本油料产业发展潜力与对策分析[J]. 林产工业,2023,60(1):64-68.
[5]陈雷.阜阳薄壳山核桃产业化发展存在的问题及建议[J]. 山西果树,2019(3):28-29.
[6]Vandermeer J H.The ecology of intercropping[M]. Cambridge,UK:Cambridge University Press,1989:162-175.
[7]Lithourgidis A,Dordas C,Damalas C A,et al. Annual intercrops:an alternative pathway for sustainable agriculture[J]. Australian Journal of Crop Science,2011,5:396-410.
[8]王晶晶,唐菲,高俊兰,等. 江淮丘陵地区薄壳山核桃林农复合模式综合效益分析[J]. 安徽农业科学,2025,53(2):124-126.
[9]Wang R Y,Liu C Y,Bie X S,et al. Pecan-medicinal crops intercropping improved soil fertility and promoted interactions between soil microorganisms and metabolites[J]. Chemical and Biological Technologies in Agriculture,2024,11(1):162.
[10]马骧,朱灿灿.苏南丘陵地区薄壳山核桃幼林与白茶复合栽培技术[J]. 现代农业科技,2016(17):62-63.
[11]翟敏,张楠,李晓储,等. 薄壳山核桃林农间种幼龄效益[J]. 林业科技通讯,2022(3):77-80.
[12]鲍士旦.土壤农化分析[M]. 3版.北京:中国农业出版社,2000:30-354.
[13]关松荫. 土壤酶及其研究法[M]. 北京:农业出版社,1986:274-339.
[14]Bolger A M,Lohse M,Usadel B. Trimmomatic:a flexible trimmer for Illumina sequence data[J]. Bioinformatics,2014,30(15):2114-2120.
[15]Martin M. Cutadapt removes adapter sequences from high-throughput sequencing reads[J]. EMBnet Journal,2011,17(1):10.
[16]Edgar R C. UPARSE:highly accurate OTU sequences from microbial amplicon reads[J]. Nature Methods,2013,10(10):996-998.
[17]Callahan B J,McMurdie P J,Rosen M J,et al. DADA2:high-resolution sample inference from Illumina amplicon data[J]. Nature Methods,2016,13(7):581-583.
[18]Ondov B D,Bergman N H,Phillippy A M.Interactive metagenomic visualization in a Web browser[J]. BMC Bioinformatics,2011,12(1):385.
[19]Caporaso J G,Kuczynski J,Stombaugh J,et al. QIIME allows analysis of high-throughput community sequencing data[J]. Nature Methods,2010,7(5):335-336.
[20]Zhang X P,Gao G B,Wu Z Z,et al. Responses of soil nutrients and microbial communities to intercropping medicinal plants in moso bamboo plantations in subtropical China[J]. Environmental Science and Pollution Research,2020,27(2):2301-2310.
[21]徐永杰,吴强盛,汪芳玲,等. 油茶和薄壳山核桃间作对土壤养分和真菌多样性的影响[J]. 中南林业科技大学学报,2024,44(1):28-36.
[22]李华健,邓国军,罗贞媛,等. 间作模式对红壤理化性质和大豆品质的影响[J]. 中国农学通报,2024,40(26):8-14.
[23]叶春秀,苏康妮,马雨荷,等. 核桃‖玉米间作对核桃果实品质及产量的影响[J]. 西北农业学报,2022,31(10):1365-1373.
[24]刘传和,贺涵,邵雪花,等. 不同覆盖处理对菠萝园土壤代谢物和细菌群落结构的影响[J]. 生物技术通报,2024,40(7):247-258.
[25]Tang B,Xu H P,Song F M,et al. Effects of heavy metals on microorganisms and enzymes in soils of lead-zinc tailing ponds[J]. Environmental Research,2022,207:112174.
[26]Datta R.Enzymatic degradation of cellulose in soil:a review[J]. Heliyon,2024,10(1):e24022.
[27]Yang Z,Yuan Y,Guo J J,et al. Responses of soil C,N,P and enzyme activities to biological soil crusts in China:a meta-analysis[J]. Plants,2024,13(11):1525.
[28]Cantarella H,Otto R,Soares J R,et al. Agronomic efficiency of NBPT as a urease inhibitor:a review[J]. Journal of Advanced Research,2018,13:19-27.
[29]Reichert T,Rammig A,Fuchslueger L,et al. Plant phosphorus-use and-acquisition strategies in Amazonia[J]. New Phytologist,2022,234(4):1126-1143.
[30]Schoebitz M,Castillo D,Jorquera M,et al. Responses of microbiological soil properties to intercropping at different planting densities in an acidic Andisol[J]. Agronomy,2020,10(6):781.
[31]张向前,黄国勤,卞新民,等. 施氮肥与隔根对间作大豆农艺性状和根际微生物数量及酶活性的影响[J]. 土壤学报,2012,49(4):731-739.
[32]Gao N,Wen H,Shang Z W,et al. Macrogenomics reveal the effects of inter-cropping Perilla on kiwifruit:impact on inter-root soil microbiota and gene expression of carbon,nitrogen,and phosphorus cycles in kiwifruit[J]. Frontiers in Microbiology,2024,15:1349305.
[33]张萌萌,敖红,李鑫,等. 桑树‖苜蓿间作对根际土壤酶活性和微生物群落多样性的影响[J]. 草地学报,2015,23(2):302-309.
[34]代会会,胡雪峰,曹明阳,等. 豆科间作对番茄产量、土壤养分及酶活性的影响[J]. 土壤学报,2015,52(4):911-918.
[35]Tu L H,Peng Y,Chen G,et al. Direct and indirect effects of nitrogen additions on fine root decomposition in a subtropical bamboo forest[J]. Plant and Soil,2015,389(1):273-288.
[36]鲍佳书,汤玉洁,刘俊萍,等. 不同品种薄壳山核桃林地土壤微生物多样性及群落组成[J]. 中南林业科技大学学报,2022,42(9):24-36.
[37]刘彩霞,董玉红,焦如珍.森林土壤中酸杆菌门多样性研究进展[J]. 世界林业研究,2016,29(6):17-22.
[38]Ventura M,Canchaya C,Tauch A,et al. Genomics of Actinobacteria:tracing the evolutionary history of an ancient phylum[J]. Microbiology and Molecular Biology Reviews,2007,71(3):495-548.
[39]Panda A,Islam S T,Sharma G.Harmonizing prokaryotic nomenclature:fixing the fuss over phylum name flipping[J]. mBio,2022,13(3):e00970-e00922.
[40]Beimforde C,Feldberg K,Nylinder S,et al. Estimating the Phanerozoic history of the Ascomycota lineages:combining fossil and molecular data[J]. Molecular Phylogenetics and Evolution,2014,78:386-398.
[41]Elisashvili V,Kachlishvili E,Penninckx M.Effect of growth substrate,method of fermentation,and nitrogen source on lignocellulose-degrading enzymes production by white-rot basidiomycetes[J]. Journal of Industrial Microbiology & Biotechnology,2008,35(11):1531-1538.
[42]曾成城,秦芳,苏利荣,等. 核桃不同套种模式对土壤养分、微生物数量及酶活性的影响[J]. 西南农业学报,2021,34(2):258-263.
[43]Perdoná M J,Soratto R P. Arabica coffee-macadamia intercropping:yield and profitability with mechanized coffee harvesting[J]. Agronomy Journal,2020,112(1):429-440.
[44]郝海平,白红彤,夏菲,等. 茶‖山苍子间作对茶园土壤微生物群落结构的影响[J]. 中国农业科学,2021,54(18):3959-3969.
[45]Diakhaté S,Gueye M,Chevallier T,et al. Soil microbial functional capacity and diversity in a millet-shrub intercropping system of semi-arid Senegal[J]. Journal of Arid Environments,2016,129:71-79.
[46]Tian X L,Wang C B,Bao X G,et al. Crop diversity facilitates soil aggregation in relation to soil microbial community composition driven by intercropping[J]. Plant and Soil,2019,436(1/2):173-192.
[47]常小箭,张星,段志龙,等. 鼠茅草间作对猕猴桃园土壤理化性质及微生物含量的影响[J]. 果树资源学报,2025,6(2):29-34.
[48]Wang Q C,Peng X J,Yuan Y X,et al. The effect of Torreya grandis inter-cropping with Polygonatum sibiricum on soil microbial community[J]. Frontiers in Microbiology,2024,15:1487619.
[49]常亚锋,于文豪,陈厚朴,等. 种植年限对健康三七根际土壤微生物活性和功能的影响[J]. 土壤,2023,55(1):132-139.
[50]于天一,杨吉顺,吴正锋,等. 不同类型地膜对花生生长及根际细菌群落影响的比较分析[J]. 中国农业科学,2023,56(24):4842-4853.
[51]Husbands D R,Henkel T W,Bonito G,et al. New species of Xerocomus (Boletales) from the Guiana Shield,with notes on their mycorrhizal status and fruiting occurrence[J]. Mycologia,2013,105(2):422-435.
[52]Eduardo P P,Margarita V R,Roberto G O,et al. Two new species of Clavulina and the first record of Clavulina reae from temperate Abies religiosa forests in central Mexico[J]. Mycological Progress,2019,18(9):1187-1200.
[53]曾维军,王晶,杨彝华,等. 印度块菌成熟期菌塘土壤营养与微生物多样性变化的研究[J]. 西部林业科学,2022,51(4):26-33.
[54]周晓莹,梁玉,李红丽,等. 双色蜡蘑对黑松幼苗生长量及其根系形态的影响[J]. 西北植物学报,2018,38(6):1153-1159.

相似文献/References:

[1]李岳峰,居立海,张来运,等.水分胁迫下丛枝菌根对水稻/绿豆间作系统 作物生长和氮磷吸收的影响[J].江苏农业科学,2013,41(04):58.
[2]刘广勤,俞卫东,曹仁勇,等.薄壳山核桃食药用价值及加工利用研究进展[J].江苏农业科学,2014,42(12):302.
 Liu Guangqin,et al.Research progress on edible and medicinal value of Carya illinoensis and its processing and utilization[J].Jiangsu Agricultural Sciences,2014,42(13):302.
[3]王新华,尚赏,郭书亚,等.2BX型玉米/甘薯间作系统优势分析[J].江苏农业科学,2014,42(10):106.
 Wang Xinhua,et al.Superiority analysis of 2BX type corn/sweet potato intercropping system[J].Jiangsu Agricultural Sciences,2014,42(13):106.
[4]叶珺琳,郭国保,潘春香,等.间种芳香植物对蔬菜生长及虫害的影响[J].江苏农业科学,2014,42(08):143.
 Ye Junlin,et al.Effect of intercropping aromatic plants on growth and insects of vegetable[J].Jiangsu Agricultural Sciences,2014,42(13):143.
[5]刘广勤,张普娟,生静雅,等.薄壳山核桃良种特贾斯引种试验[J].江苏农业科学,2013,41(11):207.
 Liu Guanqin,et al.Introduction trial of a fine pecan cultivar “Tejas”[J].Jiangsu Agricultural Sciences,2013,41(13):207.
[6]熊军,闫海锋,韦绍丽,等.木薯+花生间作对作物光合特性、农艺性状和产量的影响[J].江苏农业科学,2016,44(06):165.
 Xiong Jun,et al.Effects of cassava and peanut intercropping on photosynthesis characteristics, agronomic traits and yield of crops[J].Jiangsu Agricultural Sciences,2016,44(13):165.
[7]付学鹏,吴凤芝,周新刚.间作防控作物土传病害的机理研究进展[J].江苏农业科学,2016,44(01):16.
 Fu Xuepeng,et al.Research progress on mechanism of plant soil-borne diseases control in intercropping system[J].Jiangsu Agricultural Sciences,2016,44(13):16.
[8]谢利,王燕芳,马超,等.棉花-孜然间作模式对土壤微生物数量及酶活性的影响[J].江苏农业科学,2015,43(10):103.
 Xie Li,et al.Effects of cotton and cumin intercropping pattern on soil microorganisms and enzyme activity[J].Jiangsu Agricultural Sciences,2015,43(13):103.
[9]齐付国,刘小飞,孙景生.不同供水水平对间作甜瓜叶片活性氧代谢及光合特性的影响[J].江苏农业科学,2015,43(09):199.
 Qi Fuguo,et al.Effects of different water treatments on active oxygen metabolism and photosynthetic characteristics of intercropping melon leaf[J].Jiangsu Agricultural Sciences,2015,43(13):199.
[10]郭素芬,兰阿峰,李丽霞.非寄主植物粗提物对小菜蛾幼虫的驱避作用[J].江苏农业科学,2015,43(04):164.
 Guo Sufen,et al.Repellent effect of crude extracts from non-host plants on diamondback moth larvae[J].Jiangsu Agricultural Sciences,2015,43(13):164.

备注/Memo

备注/Memo:
收稿日期:2025-06-27
基金项目:江西省科技厅重点研发计划“揭榜挂帅”项目(编号:20223BBF61014)。
作者简介:侯孟昕(2001—),男,江苏沛县人,硕士研究生,主要研究方向为薄壳山核桃抗性基因。E-mail:hou_mengxin@163.com。
通信作者:翟敏,硕士,高级实验师,主要从事薄壳山核桃培育相关工作。E-mail:zhaimin@jib.ac.cn。
更新日期/Last Update: 2026-07-05