[1]宋洋,王永旭,杨开宇,等.核桃APX基因家族成员鉴定及逆境胁迫表达分析[J].江苏农业科学,2026,54(16):26-34.
　Song Yang,et al.Identification of walnut APX gene family members and expression analysis under adversity stress[J].Jiangsu Agricultural Sciences,2026,54(16):26-34.
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核桃APX基因家族成员鉴定及逆境胁迫表达分析()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第54卷
期数:
2026年第16期
页码:
26-34
栏目:
作物耐干旱胁迫研究专题
出版日期:
2026-08-20

文章信息/Info

Title:
Identification of walnut APX gene family members and expression analysis under adversity stress
作者:
宋洋; 王永旭; 杨开宇; 李寒; 齐国辉; 董庆龙
河北农业大学林学院，河北保定 071000
Author(s):
Song Yang; et al
关键词:
核桃; 抗坏血酸过氧化物酶; 逆境胁迫; 基因家族; 系统进化; 表达分析
Keywords:
-
分类号:
S664.101
DOI:
-
文献标志码:
A
摘要:
抗坏血酸过氧化物酶(APX)在植物生长发育及逆境胁迫响应中起到重要作用，然而关于核桃(Juglans regia)APX基因的研究还比较少。为系统鉴定核桃APX基因家族成员，并解析其在不同逆境胁迫下的表达模式，本研究利用生物信息学方法从核桃基因组数据库筛选并鉴定出9个核桃JrAPX基因家族成员。系统进化树结果表明，JrAPX蛋白部分成员与拟南芥APX蛋白具有较高的进化保守性，暗示两者功能相似。利用转录组和实时荧光定量PCR技术检测核桃APX基因家族成员在不同逆境胁迫下的表达模式。结果表明，在胶孢炭疽菌侵染时，JrAPX1和JrAPX2表达量显著上调(P<0.05)；黑斑病侵染1 d时，JrAPX1表达量明显上调，苹果蠹蛾侵食12 h时JrAPX1和JrAPX2表达量下调，侵食24 h时表达量上调；在营养胁迫下，JrAPX2和JrAPX1表达量出现显著下调；在盐胁迫下，JrAPX6和JrAPX4的表达量出现显著下调，JrAPX2的表达量出现显著上调；在干旱胁迫下，JrAPX3的表达量显著上调，JrAPX6的表达量显著下调。综上，不同逆境胁迫下基因表达呈现特异性变化规律，其中JrAPX1、JrAPX2、JrAPX3和JrAPX6是关键响应基因。该结果为揭示核桃APX基因在逆境胁迫中的作用机制提供了重要线索，也为通过分子育种手段提高核桃抗逆性提供了重要理论基础。
Abstract:
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备注/Memo

备注/Memo:
收稿日期：2025-09-19
基金项目：河北省重点研发计划(编号：21326304D-2)；河北省现代农业产业技术体系项目(编号：HBCT2025190205)；国家重点研发子课题(编号：2022YFD1600402-02)。
作者简介：宋洋(2000—)，男，河北承德人，主要从事经济林培育方向研究。E-mail：songyang20001020@163.com。
通信作者：齐国辉，博士，教授，博士生导师，主要研究方向为经济林栽培，E-mail：bdqgh@sina.com；董庆龙，博士，副教授，主要研究方向为果树逆境胁迫和经济林栽培学等，E-mail：dong19850412@163.com。
更新日期/Last Update: 2026-08-20