[1]袁连焰,牛梦娜,徐平,等.糙皮侧耳bHLH基因家族鉴定及表达分析[J].江苏农业科学,2026,54(16):111-119.
　Yuan Lianyan,et al.Identification and expression analysis of bHLH gene family in Pleurotus ostreatus[J].Jiangsu Agricultural Sciences,2026,54(16):111-119.
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糙皮侧耳bHLH基因家族鉴定及表达分析()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第54卷
期数:
2026年第16期
页码:
111-119
栏目:
生物技术
出版日期:
2026-08-20

文章信息/Info

Title:
Identification and expression analysis of bHLH gene family in Pleurotus ostreatus
作者:
袁连焰1; 2; 牛梦娜1; 2; 徐平1; 林金盛1; 侯立娟1; 蒋宁1; 马林1; 曲绍轩1; 2; 李辉平1; 2
1.江苏省农业科学院蔬菜研究所/江苏省高效园艺作物遗传改良重点实验室，江苏南京 210014； 2.西南林业大学食用菌现代产业学院，云南昆明 650224
Author(s):
Yuan Lianyan; et al
关键词:
糙皮侧耳; 转录因子; bHLH基因家族; 表达分析
Keywords:
-
分类号:
S188
DOI:
-
文献标志码:
A
摘要:
为探究糙皮侧耳(Pleurotus ostreatus)bHLH基因家族的分子特征与表达规律，为后续深入解析其生物学功能及应用奠定基础，利用生物信息学方法预测和分析PobHLH蛋白的理化性质和亚细胞定位，分析其家族基因的染色体定位、基因结构、顺式作用元件以及遗传进化关系，并检测其在菌丝、原基、子实体等不同生长发育时期和不同部位的表达模式。结果筛选出分布在7条染色体上的10个PobHLH(PobHLH01~PobHLH10)，其编码的蛋白分子量 27.6~168.7 ku，等电点分布在5.87~9.37，均为不稳定蛋白且亲水，电子预测均位于细胞核内。对启动子分析发现，大量激素响应元件、光响应元件和少量的胁迫响应元件、生长发育元件分布其中，而且生长发育元件在PobHLH01和PobHLH02启动子中发生了缺失。系统进化分析发现10个PobHLH分属在7个基因类群。不同时期和部位的基因表达分析结果发现，F1类群成员PobHLH03和F9类群成员PobHLH04、PobHLH05在幼菇和成熟子实体的菌柄中显著高表达。糙皮侧耳全基因组鉴定出10个bHLH基因家族成员，其中PobHLH03、bHLH04和PobHLH05可能在糙皮侧耳菌柄生长发育过程中发挥重要作用。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2025-10-10
基金项目：国家食用菌现代农业产业技术体系建设专项(编号：CARS-20)；国家自然科学基金(编号：32573111)；江苏省常州市科技计划(编号：CE20232015)。
作者简介：袁连焰(2001—)，女，重庆江津人，硕士研究生，研究方向为食用菌子实体发育调控。E-mail：yly20011204@163.com。
通信作者：李辉平，博士，副研究员，研究方向为食用菌栽培和遗传育种。E-mail：hplee@jaas.ac.cn。
更新日期/Last Update: 2026-08-20