[1]鲁丹丹,梁妍,谭政委,等.牛至TCP基因家族的全基因组鉴定及其表达分析[J].江苏农业科学,2026,54(16):130-141.
 Lu Dandan,et al.Genomewide identification and expression analysis of TCP gene family in Origanum vulgare[J].Jiangsu Agricultural Sciences,2026,54(16):130-141.
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牛至TCP基因家族的全基因组鉴定及其表达分析()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第54卷
期数:
2026年第16期
页码:
130-141
栏目:
生物技术
出版日期:
2026-08-20

文章信息/Info

Title:
Genomewide identification and expression analysis of TCP gene family in Origanum vulgare
作者:
鲁丹丹; 梁妍; 谭政委; 苏小雨; 孙瑶; 余永亮; 李磊; 李春明; 乔美玉; 王利娜; 曹艺雯; 杨红旗; 梁慧珍
河南省农业科学院中药材研究所/河南省中药材资源保护与利用重点实验室,河南郑州 450002
Author(s):
Lu Dandan; et al
关键词:
牛至; TCP基因家族; 共线性分析; 亚细胞定位; 表达分析
Keywords:
-
分类号:
S188
DOI:
-
文献标志码:
A
摘要:
TCP是一种广泛分布且至关重要的植物转录调控因子,在调控植物生长发育和逆境胁迫中有重要作用。本研究基于牛至基因组数据,从全基因组水平鉴定到23个OvTCP基因不均匀地分布在牛至的15条染色体上,且其中11对基因间存在共线性关系,18个基因检测到全基因组复制或片段复制事件。蛋白理化性质分析发现,23个OvTCP蛋白氨基酸的长度、分子量、等电点各不相同,但这些蛋白都属于亲水性蛋白,且均定位于细胞核中;GFP融合蛋白表达法对OvTCP5亚细胞定位的研究验证了这一预测。氨基酸序列比对及系统进化分析将OvTCP分为CIN、PCF、TB1/CYC亚群,各亚群的Motif组成相似,但不同亚群间差异很大,每一亚群都含有其独特的Motif。启动子分析发现,OvTCP启动子中所含ABRE、Box4数量最多,表明其在牛至响应逆境胁迫及光响应中发挥重要作用。转录组数据结合qRT-PCR分析发现,OvTCP6/13/15/19在叶中表达量较高,在茎中表达量低或几乎不表达;OvTCP16在上部茎中表达量最高,在其他部位表达量很低。
Abstract:
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备注/Memo

备注/Memo:
收稿日期:2025-10-20
基金项目:国家现代农业产业技术体系资助项目(编号:CARS-21);河南省农科院优秀青年科技基金(编号:2024YQ15、2024YQ16);河南省外籍科学家工作室项目(编号:GZS2024025);河南省重点研发专项(编号:241111310200、231111110800、251111310600、251111111800)。
作者简介:鲁丹丹(1989—),女,河南周口人,硕士,助理研究员,主要从事药用植物功能基因鉴定研究。E-mail:ludandan0710@163.com。
通信作者:梁慧珍,博士,研究员,主要从事药用植物分子育种研究。E-mail:lhzh66666@163.com。
更新日期/Last Update: 2026-08-20