[1]李洋,董红秀,李媛媛.基于转录组与代谢组联合分析3种牡丹品种显蕾期花色形成机制[J].江苏农业科学,2026,54(16):244-251.
　Li Yang,et al.Mechanisms of flower color formation of three peony cultivars at apparent bud stage based on joint transcriptome and metabolome analysis[J].Jiangsu Agricultural Sciences,2026,54(16):244-251.
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基于转录组与代谢组联合分析3种牡丹品种显蕾期花色形成机制()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第54卷
期数:
2026年第16期
页码:
244-251
栏目:
园艺与林学
出版日期:
2026-08-20

文章信息/Info

Title:
Mechanisms of flower color formation of three peony cultivars at apparent bud stage based on joint transcriptome and metabolome analysis
作者:
李洋; 董红秀; 李媛媛
商丘学院风景园林与建筑学院，河南商丘 476000
Author(s):
Li Yang; et al
关键词:
牡丹; 显蕾期; 花色; 代谢组; 转录组; 类黄酮; 花青素
Keywords:
-
分类号:
S188；S685.110.1
DOI:
-
文献标志码:
A
摘要:
为明确牡丹不同花色在显蕾期的分子调控机制，进而为牡丹花色改良的分子育种提供理论基础。本研究以白雪塔(白色)、赵粉(粉色)和洛阳红(红色)等3个不同花色的牡丹品种为研究对象，采用代谢组与转录组联合分析的方法，筛选与花色形成相关的关键差异代谢物和差异基因，并通过qPCR对查尔酮合成酶(CHS)、花青素合成酶(ANS)及黄烷酮3-双加氧酶(F3H)等关键基因的表达量进行验证。结果表明，3个花色牡丹的差异代谢物和差异基因主要富集于以苯丙氨酸为起始的黄酮类/花青素及黄酮醇生物合成通路。转录组测序结果表明，CHS和ANS在白色花中表达量最高，在红色花和粉色花中依次降低，而F3H在红色花中表达量最高，该结果在qPCR中也得到了验证。其中，红色花(洛阳红)中类黄酮3′-羟化酶(F3′H)与F3H基因表达量最高，并与槲皮素和圣草酚相对丰度较高相对应；矢车菊素-3-阿拉伯糖苷阳离子的相对丰度为白色花的56.09倍，这些基因和代谢物可能与红色形成密切相关；粉色花（赵粉）中，芹菜素、异槲皮苷、木樨草素及矢车菊素-3-O-β-D-桑布双糖苷等代谢物相对丰度较高；白色花(白雪塔)的形成可能与特定黄酮类物质/黄酮醇的含量及其与花青素的互作相关。本研究结果为牡丹花色分子育种提供了F3′H、F3H等候选基因及理论依据。
Abstract:
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备注/Memo

备注/Memo:
收稿日期：2025-08-04
基金项目：2025年商丘市科技攻关项目。
作者简介：李洋(1995—)，女，河南南阳人，硕士，讲师，从事研究方向为景观设计、林业生态、遗传多样性的研究。E-mail：ly622721@126.com。
更新日期/Last Update: 2026-08-20