[1]夏彦飞,郑伟,苏盼盼,等.枯草芽孢杆菌OKB105菌株不同培养基发酵上清液的杀线活性[J].江苏农业科学,2014,42(12):144-147.
　Xia Yanfei,et al.Nematicidal activities of different fermentation cultured supernatants of Bacillus subtilis strain OKB105 against plant-parasitic nematodes[J].Jiangsu Agricultural Sciences,2014,42(12):144-147.
点击复制

枯草芽孢杆菌OKB105菌株不同培养基发酵上清液的杀线活性()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第42卷
期数:
2014年12期
页码:
144-147
栏目:
植物保护
出版日期:
2014-12-25

文章信息/Info

Title:
Nematicidal activities of different fermentation cultured supernatants of Bacillus subtilis strain OKB105 against plant-parasitic nematodes
作者:
夏彦飞1;  郑伟1;  苏盼盼1;  乔俊卿2;  王建华3;  黄晓博1
1.河南科技大学，河南洛阳 471003； 2.江苏省农业科学院植物保护研究所，江苏南京 210014；3.江苏省宜兴市金丰农产品有限责任公司，江苏宜兴 214200
Author(s):
Xia Yanfei; et al
关键词:
枯草芽孢杆菌; 植物寄生线虫; 培养基; 发酵滤液; 杀线活性; 拮抗性
Keywords:
-
分类号:
S432.4+5
DOI:
-
文献标志码:
A
摘要:
采用室内离体测定方法，测试枯草芽孢杆菌OKB105菌株在不同培养基（Landy、LB、λ肉汤、TY、超级肉汤、胰化蛋白胨肉汤、TYGPN、A、M63、M9）的发酵滤液中对植物寄生线虫［南方根结线虫(Meloidogyne incognita) 2龄幼虫、程氏伞滑刃线虫（Bursaphelenchus chengi）、豆伞滑刃线虫（B. doui）、拟松材线虫（B. mucronatus）］的生物活性，以期获得具有较高防效的发酵培养基。结果表明：OKB105菌株的培养滤液分别处理植物寄生线虫6 h后，对南方根结线虫2龄幼虫防效较佳的发酵培养基是TYGPN、LB、LANDY、TY、胰化蛋白胨肉汤、超级肉汤等6个培养基；对豆伞滑刃线虫防效较佳的是TYGPN和TY 等2个培养基；对豆伞滑刃线虫、拟松材线虫防效最佳的是TYGPN培养基；OKB105菌株TYGPN培养基发酵上清液对4种标靶线虫都具有较强的拮抗性。
Abstract:
-

参考文献/References:

［1］Oka Y，Koltai H，Bar-Eyal M，et al. New strategies for the control of plant-parasitic nematodes［J］. Pest Management Science，2000，56(11)：983-988.
［2］Schneider S M，Rosskopf E N，Leesch J G，et al. Research on alternatives to methyl bromide：pre-plant and post-harvest［J］. Pest Manag Sci，2003，59：814-826.
［3］Stirling G R. Biological control of plant parasitic nematode：progress，problems and prospects［M］. Wallington：CAB International，1991.
［4］Siddiqui Z A，Mahmood I. Role of bacteria in the management of plant parasitic nematodes［J］. Bioresource Technol，1999，69：167-179.
［5］Kerry B R. Rhizosphere interactions and exploitation of microbial agents for the biological control of plant-parasitic nematodes［J］. Annu Rev Phytopathol，2000，38：423-441.
［6］Meyer S L F. United States department of agriculture-agricultural research service research programs on microbes for management of plant-parasitic nematodes［J］. Pest Manag Sci，2003，59：665-670.
［7］Tian B Y，Yang J K，Zhang K Q.Bacteria used in the biological control of plant-parasitic nematodes：populations，mechanisms of action，and future prospects［J］. FEMS Microbiol Ecol，2007，61：197-213.
［8］Obagwu J，Korsten L. Integrated control of citrus green and blue molds using Bacillus subtilis in combination with sodium bicarbonate or hot water［J］. Postharvest Biology and Technology，2003，28(1)：187-194.
［9］Niu Q H，Huang X W，Tian B Y，et al. Bacillus sp B16 kills nematodes with a serine protease identified as a pathogenic factor［J］. Applied Microbiology and Biotechnology，2006，69(6)：722-730.
［10］Niu Q H，Huang X W，Zhang L，et al. A Trojan horse mechanism of bacterial pathogenesis against nematodes［J］. Proceedings of the National Academy of Sciences of the United States of America，2010，107(38)：16631-16636.
［11］Chen Z X，Dickson D W. Review of pasteuria penetrans：biology，ecology，and biological control potential［J］. Journal of Nematology，1998，30（3）：313-340.
［12］Xiao T J，Chen F，Gao C，et al. Bacillus cereus X5 enhanced bio-organic fertilizers effectively control root-knot nematodes(Meloidogyne sp.)［J］. Pedosphere，2013，23(2)：160-168.
［13］Xia Y F，Xie S S，Ma X，et al. The purL gene of Bacillus subtilisis associated with nematici- dal activity ［J］. FEMS Microbiol Lett，2011，322：99-107.
［14］丁国春，付鹏，李红梅，等. 枯草芽孢杆菌AR11菌株对南方根结线虫的生物防治［J］.南京农业大学学报，2005，28（2）：46-49.
［15］朱丽梅，吴小芹，陈江天，等. 一株毒杀松材线虫的解淀粉芽孢杆菌活性物质的基本性质［J］.中国生物防治，2009，25（4）：359-363.
［16］许志刚. 普通植物病理学实验指导［M］.北京：农业出版社，1993：62-65.
［17］Landy M，Warren G H，Roseman S B. Bacillomycin，an antibiotic from Bacillus subtilis active against pathogenic fungi［J］. Proc Soc Exp Biol Med，1948，67：539-541.
［18］Ausubel F M. 精编分子生物学实验指南［M］.颜子颖，王海林，译.北京：科学出版社，1998：2-3.
［19］Huang X W，Tian B Y，Niu Q H，et al. An extracellular protease from Brevibacillus laterosporus G4 without parasporal crystals can serve as a pathogenic factor in infection of nematodes［J］. Research in Microbiology，2005，156(5/6)：719-727.
［20］Ali N I，Siddiqui I A，Shaukat S S，et al. Nematicidal activity of some strains of Pseudomonas spp［J］. Soil Biology & Biochemistry，2002，34：1051-1058.
［21］Guo J H，Qi H Y，Guo Y H，et al. Biocontrol of tomato wilt by plant growth-promoting rhizobacteria［J］. Biological Control，2004，29：66-72.
［22］Siddiqui Z A. PGPR：biocontrol and biofertilization［M］. New York：Springer，2005：111-142.
［23］Wang S，Wu H J，Zhan J，et al. The role of synergistic action and molecular mechanism in the effect of genetically engineered strain Bacillus subtilis OKBHF in enhancing tomato growth and cucumber mosaic virus resistance［J］. Bio Control，2011，56(1)：113-121.
［24］Li B，Xie G L，Soad A，et al. Suppression of Meloidogyne javanica by antagonistic and plant growth-promoting rhizobacteria［J］. J Zhejiang Univ Sci，2005，6(6)：496-501.
［25］田宝玉，黄薇，江贤章，等. 植物寄生线虫生防细菌及其作用机制研究［J］.生物技术，2009，19（4）：90-93.

相似文献/References:

[1]郭凤柳,张海颖,李勇,等.马铃薯疮痂病拮抗菌株B1的鉴定及防效测定[J].江苏农业科学,2013,41(05):90.
　Guo Fengliu,et al.Identification and control efficiency of biocontrol strain B1 for potato scab pathogens[J].Jiangsu Agricultural Sciences,2013,41(12):90.
[2]刘琴,王艳,徐健,等.枯草芽孢杆菌Bs-1013的抑菌活性及其包衣对黄瓜发芽生长的影响[J].江苏农业科学,2013,41(11):117.
　Liu Qin,et al.Antibiotic activity of Bacillus subtilis Bs-1013 and effect of its coating agent on seed germination and growth of cucumber[J].Jiangsu Agricultural Sciences,2013,41(12):117.
[3]张婷婷,马磊.蜡样芽孢杆菌启动子片段克隆及转录活性分析[J].江苏农业科学,2014,42(04):25.
　Zhang Tingting,et al.Cloning and transcription of promoter fragment in Bacillus cereus[J].Jiangsu Agricultural Sciences,2014,42(12):25.
[4]关一鸣,潘晓曦,王莹,等.哈茨木霉菌、枯草芽孢杆菌对人参灰霉病和根腐病病原菌的拮抗作用[J].江苏农业科学,2014,42(05):123.
　Guan Yiming,et al.Antagonism of Trichoderma harzianum and Bacillus subtilis against Botrytis cinerea Pers. and Fusarium solani on ginseng[J].Jiangsu Agricultural Sciences,2014,42(12):123.
[5]李旭,李雪,胡秀彩,等.鲫鱼肠道中枯草芽孢杆菌的分离鉴定及药敏试验[J].江苏农业科学,2014,42(07):244.
　Li Xu,et al.Isolation，identification and antibiotic susceptibility testing of Bacillus subtilis in Crucian carp intestine[J].Jiangsu Agricultural Sciences,2014,42(12):244.
[6]陈忠杰,胡燕.枯草芽孢杆菌对板栗采后黑斑病的抑制效果[J].江苏农业科学,2015,43(01):256.
　Chen Zhongjie,et al.Inhibitory effect of Bacillus subtilis on Chinese chestnut postharvest black rot[J].Jiangsu Agricultural Sciences,2015,43(12):256.
[7]谢鹏,付胜勇,常玲玲,等.枯草芽孢杆菌对乳鸽生长性能、小肠形态和结直肠菌群的影响[J].江苏农业科学,2015,43(06):190.
　Xie Peng,et al.Effects of Bacillus subtilis on growth performance，intestinal morphology and colorectal microbiota of pigeon squabs[J].Jiangsu Agricultural Sciences,2015,43(12):190.
[8]范瑛阁,李 莎,赵 静,等.枯草芽孢杆菌H1和H2对黄瓜的促生作用[J].江苏农业科学,2015,43(07):158.
　Fan Yinge,et al.Promoting effect of Bacillus subtilis H1 and H2 on cucumber[J].Jiangsu Agricultural Sciences,2015,43(12):158.
[9]林志伟,肖亚静,郭春兰,等.枯草芽孢杆菌21代谢物对大豆茄镰孢菌的抑菌机制[J].江苏农业科学,2015,43(11):175.
　Lin Zhi Wei,et al.Primary study on restrain mechanism of Bacillus subtilis strain 21 metabolite to soybean Fusarium solani[J].Jiangsu Agricultural Sciences,2015,43(12):175.
[10]伍善东,刘冬华,郭照辉,等.1株高效解无机磷细菌JP-7的分离、鉴定及溶磷能力分析[J].江苏农业科学,2015,43(12):374.
　Wu Shandong,et al.Isolation and identification of a high efficiency inorganic phosphorus-dissolving bacteria from soil and its analysis of phosphate solubilization[J].Jiangsu Agricultural Sciences,2015,43(12):374.

备注/Memo

备注/Memo:
收稿日期：2014-03-25
基金项目：国家自然科学基金（编号：31201558）；河南科技大学博士启动基金（编号：09001590）；河南科技大学试验技术开发基金（编号：SY1213023）；河南科技大学SRTP项目（编号：2013257）。
作者简介：夏彦飞（1980—），男，安徽砀山人，博士研究生，讲师，从事植物寄生线虫的生物防治研究。Tel：（0379）64282345；E-mail：xyfwcc@163.com。
更新日期/Last Update: 2014-12-25