[1]王承克,陈丹,张俊俊,等.免标记法快速检测啶虫脒的研究[J].江苏农业科学,2016,44(08):324-327.
　Wang Chengke,et al.Study on rapid detection of acetamiprid using label-free method[J].Jiangsu Agricultural Sciences,2016,44(08):324-327.
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免标记法快速检测啶虫脒的研究()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第44卷
期数:
2016年08期
页码:
324-327
栏目:
加工与检测
出版日期:
2016-08-25

文章信息/Info

Title:
Study on rapid detection of acetamiprid using label-free method
作者:
王承克;  陈丹;  张俊俊;  陆峰
江苏大学食品与生物工程学院，江苏镇江 212032
Author(s):
Wang Chengke; et al
关键词:
啶虫脒; 纳米金; 适配体; 酶标仪; 检测
Keywords:
-
分类号:
O657.32
DOI:
-
文献标志码:
A
摘要:
发展了一种利用纳米金探针，免标记法检测啶虫脒的方法。本方法基于适配体能够使纳米金在NaCl溶液中保持一定的稳定性，当啶虫脒存在时，由于啶虫脒与适配体特异性的相互作用，使纳米金稳定性降低，在NaCl存在时发生聚集的原理，根据溶液吸收光谱630 nm波长处吸光度值的改变，利用酶标仪实现了啶虫脒的快速定量检测。检测线性范围为2~20 nmol/L，检测限为2 nmol/L，检测过程在5 min内即可完成。本方法还成功用于实际水样(校园湖水)中啶虫脒的检测。结果表明此方法具有较好的选择性和较高的灵敏度，对解决农产品和食品中农药残留等食品安全问题具有重要意义。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2015-06-30
基金项目：国家自然科学基金(编号：21305032)；中国博士后科研基金(编号：2014M551522)；江苏省博士后科研基金(编号：1402073B)。
作者简介：王承克(1984—)，男，河南平顶山人，博士，讲师，硕士生导师，主要从事食品安全分析研究。Tel：(0511)88780201；E-mail：wangck@ujs.edu.cn。
更新日期/Last Update: 2016-08-25