[1]张月亮,刘宝生,王利华,等.灰飞虱共生真菌P450过量表达介导的吡虫啉抗药性[J].江苏农业科学,2021,49(3):105-109.
　Zhang Yueliang,et al.Imidacloprid resistance mediated by overexpression of symbiotic fungus P450 in Laodelphax striatellus[J].Jiangsu Agricultural Sciences,2021,49(3):105-109.
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灰飞虱共生真菌P450过量表达介导的吡虫啉抗药性()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第49卷
期数:
2021年第3期
页码:
105-109
栏目:
植物保护
出版日期:
2021-02-05

文章信息/Info

Title:
Imidacloprid resistance mediated by overexpression of symbiotic fungus P450 in Laodelphax striatellus
作者:
张月亮1; 刘宝生1; 王利华1; 侍甜2; 方继朝1
1.江苏省农业科学院植物保护研究所，江苏南京 210014； 2.南京市白蚁防治服务中心，江苏南京 210004
Author(s):
Zhang Yueliang; et al
关键词:
灰飞虱; 共生真菌; P450; 吡虫啉; 过量表达; 抗药性
Keywords:
-
分类号:
S435.11
DOI:
-
文献标志码:
A
摘要:
昆虫共生菌广泛参与寄主昆虫抗药性的形成，但对其抗性产生的分子机制的研究相对较少。本研究首先在室内敏感品系基础上建立了灰飞虱抗、感吡虫啉品系，利用现有灰飞虱转录组数据库，序列验证并定量分析了15条灰飞虱共生真菌P450基因，与敏感品系相比，发现其中10条P450基因在吡虫啉抗性品系中显著过量表达(LsSFP450-2、LsSFP450-8、LsSFP450-14、LsSFP450-12、LsSFP450-4、LsSFP450-5、LsSFP450-10、LsSFP450-7、LsSFP450-15、LsSFP450-9)，由此推断灰飞虱共生真菌P450解毒代谢途径同样是介导吡虫啉抗药性的潜在因子，此结果为宿主共生菌介导杀虫剂抗性研究及田间害虫有效化学防控提供了新理论视角。
Abstract:
-

参考文献/References:

［1］Giraudo M，Unnithan G C，le Goff G，et al. Regulation of cytochrome P450 expression in Drosophila：genomic insights［J］. Pesticide Biochemistry and Physiology，2010，97(2)：115-122.
［2］Xu Z B，Zou X P，Zhang N，et al. Detoxification of insecticides，allechemicals and heavy metals by glutathione S-transferase SlGSTE1 in the gut of Spodoptera litura［J］. Insect Science，2015，22(4)：503-511.
［3］Zhang Y L，Guo H F，Yang Q，et al. Overexpression of a P450 gene (CYP6CW1) in buprofezin-resistant Laodelphax striatellus (Fallén)［J］. Pesticide Biochemistry and Physiology，2012，104(3)：277-282.
［4］Zhang Y L，Wang L H，Guo H F，et al. A transcriptome-based screen of carboxylesterase-like genes that are involved in chlorpyrifos resistance in Laodelphax striatellus (Fallén)［J］. Pesticide Biochemistry and Physiology，2012，104(3)：224-228.
［5］Liu Z，Williamson M S，Lansdell S J，et al. A nicotinic acetylcholine receptor mutation conferring target-site resistance to imidacloprid in Nilaparvata lugens (brown planthopper)［J］. Proceedings of the National Academy of Sciences，2005，102(24)：8420-8425.
［6］Nakao T，Kawase A，Kinoshita A，et al. The A2′ N mutation of the RDL γ-aminobutyric acid receptor conferring fipronil resistance in Laodelphax striatellus(Hemiptera：Delphacidae)［J］. Journal of Economic Entomology，2011，104(2)：646-652.
［7］Ranson H，Jensen B，Vulule J M，et al. Identification of a point mutation in the voltage-gated sodium channel gene of Kenyan Anopheles gambiae associated with resistance to DDT and pyrethroids［J］. Insect Molecular Biology，2000，9(5)：491-497.
［8］Suzuki Y，Shiotsuki T，Jouraku A，et al. Benzoylurea resistance in western flower thrips Frankliniella occidentalis (Thysanoptera：Thripidae)：the presence of a point mutation in chitin synthase 1［J］. Journal of Pesticide Science，2017，42(3)：93-96.
［9］Troczka B，Zimmer C T，Elias J，et al. Resistance to diamide insecticides in diamondback moth，Plutella xylostella (Lepidoptera：Plutellidae) is associated with a mutation in the membrane-spanning domain of the ryanodine receptor［J］. Insect Biochemistry and Molecular Biology，2012，42(11)：873-880.
［10］Zhang Y L，Li S，Xu L，et al. Overexpression of carboxylesterase-1 and mutation (F439H) of acetylcholinesterase-1 are associated with chlorpyrifos resistance in Laodelphax striatellus［J］. Pesticide Biochemistry and Physiology，2013，106(1/2)：8-13.
［11］Zhang Y L，Han Y C，Yang Q，et al. Resistance to cycloxaprid in Laodelphax striatellus is associated with altered expression of nicotinic acetylcholine receptor subunits［J］. Pest Management Science，2018，74(4)：837-843.
［12］Douglas A E. The B vitamin nutrition of insects：the contributions of diet，microbiome and horizontally acquired genes［J］. Current Opinion in Insect Science，2017，23：65-69.
［13］Vilcinskas A. Evolutionary plasticity of insect immunity［J］. Journal of Insect Physiology，2013，59(2)：123-129.
［14］Gressel J. Microbiome facilitated pest resistance：potential problems and uses［J］. Pest Management Science，2018，74(3)：511-515.
［15］Pang R，Chen M，Yue L，et al. A distinct strain of Arsenophonus symbiont decreases insecticide resistance in its insect host［J］. PLoS Genetics，2018，14(10)：e1007725.
［16］周益军，刘海建，王贵珍，等. 灰飞虱携带的水稻条纹病毒免疫检测［J］. 江苏农业科学，2004，32(1)：50-51.
［17］陈思宏，程兆榜，马学文，等. 江苏和云南地区水稻条纹叶枯病流行差异分析［J］. 江苏农业科学，2013，41(3)：88-90.
［18］李硕，王世娟，訾金燕，等. 灰飞虱从离体病叶快速获得水稻条纹病毒的方法［J］. 江苏农业学报，2014，30(2)：449-451.
［19］王利华，方继朝，刘宝生. 几类杀虫剂对灰飞虱的相对毒力及田间种群的抗药性现状［J］. 昆虫学报，2008，51(9)：930-937.
［20］潘以楼，陈啸寅，朱桂梅，等. 镇江地区褐飞虱种群对吡虫啉的抗药性及其治理［J］. 江苏农业科学，2008，36(4)：122-123.
［21］Liu Z W，Han Z J，Wang Y C，et al. Selection for imidacloprid resistance in Nilaparvata lugens：cross-resistance patterns and possible mechanisms［J］. Pest Management Science，2003，59(12)：1355-1359.
［22］Livak K J，Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method［J］. Methods，2001，25(4)：402-408.
［23］Xia X M，Zheng D D，Zhong H Z，et al. DNA sequencing reveals the midgut microbiota of diamondback moth，Plutella xylostella (L.) and a possible relationship with insecticide resistance［J］. PLoS One，2013，8(7)：e68852.
［24］Kikuchi Y，Hayatsu M，Hosokawa T，et al. Symbiont-mediated insecticide resistance［J］. Proceedings of the National Academy of Sciences，2012，109(22)：8618-8622.
［25］李娜，陈建明，张珏锋，等. 褐飞虱共生菌抗感吡虫啉菌株体内抗氧化酶活性的比较［J］. 植物保护学报，2011，38(3)：258-264.
［26］Cai T W，Zhang Y H，Liu Y，et al. Wolbachia enhances expression of NlCYP4CE1 in Nilaparvata lugens in response to imidacloprid stress［J］. Insect Science，2020，28(2)：12834.
［27］Balabanidou V，Kampouraki A，MacLean M，et al. Cytochrome P450 associated with insecticide resistance catalyzes cuticular hydrocarbon production in Anopheles gambiae［J］. Proceedings of the National Academy of Sciences，2016，113(33)：9268-9273.
［28］Liu N N，Li M，Gong Y H，et al. Cytochrome P450s-their expression，regulation，and role in insecticide resistance［J］. Pesticide Biochemistry and Physiology，2015，120：77-81.
［29］Feyereisen R. Insect P450 inhibitors and insecticides：challenges and opportunities［J］. Pest Management Science，2015，71(6)：793-800.
［30］王灵军，叶昌林，郑明辉，等. 细胞色素 P450 介导的果蝇抗药性研究进展［J］. 基因组学与应用生物学，2019(3)：1304-1309.
［31］Zhang Y C，Liu B S，Zhang Z C，et al. Differential expression of P450 genes and nAChR subunits associated with imidacloprid resistance in Laodelphax striatellus (Hemiptera：Delphacidae)［J］. Journal of Economic Entomology，2018，111(3)：1382-1387.

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备注/Memo

备注/Memo:
收稿日期：2020-11-19
基金项目：国家自然科学基金面上项目(编号：31972308)；江苏省农业科技自主创新资金［编号：CX (20) 1004］。
作者简介：张月亮(1983—)，男，河南通许人，博士，副研究员，主要从事昆虫毒理研究。E-mail：moonjaas@126.com。
通信作者：方继朝，博士，研究员，主要从事水稻害虫防控技术研究。E-mail：fangjc @jaas.ac.cn。
更新日期/Last Update: 2021-02-05