|本期目录/Table of Contents|

[1]王茜月,管飘萍,陈诗涵,等.1株溶血色杆菌的分离鉴定与基因组分析[J].江苏农业科学,2022,50(12):42-50.
　Wang Xiyue,et al.Isolation， identification and genome analysis of a strain of Chromobacterium haemolyticum[J].Jiangsu Agricultural Sciences,2022,50(12):42-50.
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1株溶血色杆菌的分离鉴定与基因组分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第50卷
期数:
2022年第12期
页码:
42-50
栏目:
生物技术
出版日期:
2022-06-20

文章信息/Info

Title:
Isolation， identification and genome analysis of a strain of Chromobacterium haemolyticum
作者:
王茜月1; 管飘萍1; 2; 陈诗涵1; 2; 黄紫贝1; 2; 郭鑫1; 2; 赵以恒1; 罗健雅1; 王欣宇1; 崔颢月1; 刘金彪1; 王海燕3; 刘文博1; 2
1.扬州大学兽医学院，江苏扬州 225009； 2.江苏省动物重要疫病与人兽共患病防控协同创新中心，江苏扬州 225009；3.江苏农牧科技职业学院，江苏泰州225300
Author(s):
Wang Xiyue; et al
关键词:
池塘; 溶血色杆菌; 全基因组序列; 比较基因组分析
Keywords:
-
分类号:
S182
DOI:
-
文献标志码:
A
摘要:
从池塘中分离、纯化了1株具有极强溶血活性的革兰氏阴性杆菌BB2，在TSA培养基上菌落呈灰色、半透明圆形，经16S rRNA基因序列分析确定为色杆菌属，但无法确定到种；进一步全基因组测序结果表明，该菌株与GenBank中唯一的1株稻根色杆菌基因组同源性最高，但生物学特性又不一致。文献逆索发现其命名为稻根色杆菌主要是由于其分离于水稻根部，这对分类造成了困惑。最终依据细菌形态特征、生理生化特性、16S rRNA和全基因组序列分析及文献分析等综合信息，确定分离菌BB2为溶血色杆菌，这提醒研究者们在细菌鉴定时应谨慎使用GenBank数据序列数据，一定要对相关参考文献进行全面认真研读，获取必要的证据，为最终分类提供科学依据。本研究也为进一步研究该分离株的生物学特性和应用提供了参考和基础。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2021-08-16
基金项目：江苏高校优势学科建设工程(2018)。
作者简介：王茜月(1999—)，硕士，江苏泰州人，主要从事传染病流行病学研究。E-mail：Bioyusy@outlook.com。
通信作者：刘文博(1975—)，男，博士，副教授，主要从事传染病防控。E-mail：lwb@yzu.edu.cn。
更新日期/Last Update: 2022-06-20