|本期目录/Table of Contents|

[1]孔令娟,黄钰淇,孙胜,等.冠菌素提高番茄对番茄黄化曲叶病毒抗性的分析[J].江苏农业科学,2024,52(11):116-122.
　Kong Lingjuan,et al.Analysis of coronatine improving tomato resistance to tomato yellow leaf curl virus[J].Jiangsu Agricultural Sciences,2024,52(11):116-122.
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冠菌素提高番茄对番茄黄化曲叶病毒抗性的分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第52卷
期数:
2024年第11期
页码:
116-122
栏目:
植物保护
出版日期:
2024-06-05

文章信息/Info

Title:
Analysis of coronatine improving tomato resistance to tomato yellow leaf curl virus
作者:
孔令娟1; 黄钰淇2; 孙胜3; 王鹏2; 3; 耿雪青2
1.上海市农业技术推广服务中心，上海 201103； 2.上海交通大学农业与生物学院，上海 200240；
3.山西农业大学园艺学院，山西晋中 030801
Author(s):
Kong Lingjuan; et al
关键词:
冠菌素; 番茄; 番茄黄化曲叶病毒; 基因表达; 抗性反应
Keywords:
-
分类号:
S436.412.1+1
DOI:
-
文献标志码:
A
摘要:
番茄黄化曲叶病毒(TYLCV)在生产上是一种毁灭性病害，而目前针对该病害尚无有效的防治措施。冠菌素可在植物抗逆反应中发挥一定作用，然而冠菌素提高番茄对TYLCV抗性的研究还鲜有报道。为探究冠菌素提高番茄抗番茄黄化曲叶病的机制，以番茄感病品种浦粉一号为试验材料，在盆栽试验中，通过qPCR的方法来检测叶片中TYLCV病毒含量，分别采用氮蓝四唑还原法、愈创木酚比色法和紫外吸收法测定超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)的活性，通过实时荧光定量PCR测定抗性相关基因表达量。在田间试验中，利用卷尺、游标卡尺等测定番茄植株叶片数、茎粗、开展度，并统计病情指数和植株发病率。结果表明，冠菌素处理显著降低TYLCV含量约83.5%(P<0.000 1)，显著增加了POD、SOD的活性(P<0.0001、P<0.05)，上调编码叶绿素a/b结合蛋白基因Cab-1A和Cab-1D，诱导番茄抗病信号通路上FLS2和PR-1a1上调表达。田间试验结果表明，冠菌素处理降低了植株平均病情指数和发病率，并显著增加了番茄植株的开展度(P<0.000 1)。综上所述，冠菌素可以降低番茄植株叶片中TYLCV含量，影响番茄抗氧化酶活性并增加抗性相关基因的表达量。研究结果可为冠菌素在番茄抗TYLCV过程中的应用提供理论依据。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2023-07-04
基金项目：上海市科技兴农技术创新项目(编号：2021-02-08-00-12-F00799)；上海市科委一带一路国际交流合作项目(编号：19390743400)。
作者简介：孔令娟(1977—)，女，山西汾阳人，硕士，高级农艺师，主要从事蔬菜新品种、新技术的示范与推广。E-mail：31459841@qq.com。
通信作者：王鹏，硕士研究生，主要从事设施蔬菜栽培生理学研究，E-mail：2283383845@qq.com；耿雪青，博士，副研究员，主要从事番茄与不同病原物互作机制以及番茄逆境激素信号途径研究，E-mail：xqgeng@sjtu.edu.cn。
更新日期/Last Update: 2024-06-05