|本期目录/Table of Contents|

[1]陈银霞,王志泽,冯成蒿,等.番茄抗根结线虫病Mi基因探索与WRKY转录因子参与抗病调控的研究进展[J].江苏农业科学,2025,53(4):16-22.
　Chen Yinxia,et al.Research progress of Mi gene against root-knot nematode disease in tomato and WRKY transcription factors involved in disease resistance regulation[J].Jiangsu Agricultural Sciences,2025,53(4):16-22.
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番茄抗根结线虫病Mi基因探索与WRKY转录因子参与抗病调控的研究进展(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第53卷
期数:
2025年第4期
页码:
16-22
栏目:
专论与综述
出版日期:
2025-02-20

文章信息/Info

Title:
Research progress of Mi gene against root-knot nematode disease in tomato and WRKY transcription factors involved in disease resistance regulation
作者:
陈银霞; 王志泽; 冯成蒿; 周闯闯; 聂蔚丹; 王超楠; 杜崇
新疆农业大学园艺学院，新疆乌鲁木齐 830052
Author(s):
Chen Yinxia; et al
关键词:
番茄; Mi基因; 根结线虫; WRKY转录因子
Keywords:
-
分类号:
S436.412.1+9
DOI:
-
文献标志码:
A
摘要:
根结线虫病作为世界范围内危害植物最为广泛的土传病害之一，给农作物生产造成了巨大的经济损失。番茄作为新疆红色支柱产业，其设施生产饱受线虫病的侵害。目前，生产上对根结线虫病的防治多以化学防治为主，但药剂的频繁使用给生态环境带来了巨大压力。近年来，生物防治的绿色可持续优势逐渐扩大，但生防资源少、田间防治效果差异大等缺陷也掣肘了其应用。优质基因资源的挖掘与利用，仍是从根本上解决番茄抗病的最佳策略。本文首先介绍番茄Mi基因家族中已发掘的10个成员在分子层面的研究进展，阐释番茄抗根结线虫病的现有分子机理；Mi基因可使用的抗源单一、抗病范围有限及土壤温度等条件的制衡，阻碍了优质番茄种质的抗性改良，更多非R基因的挖掘变得尤为重要。WRKY转录因子在作物生物胁迫调控方面扮演重要角色，本文综述近年来WRKY在作物防卫根结线虫病方面的研究应用，以期为后续番茄从不同层面并避免完全依赖Mi基因的抗病育种提供新思路。
Abstract:
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参考文献/References:

［1］Dong J X，Chen C H，Chen Z X. Expression profiles of the Arabidopsis WRKY gene superfamily during plant defense response［J］. Plant Molecular Biology，2003，51(1)：21-37.
［2］Pereira-Carvalho R C，Boiteux L S，Fonseca M E N，et al. Multiple resistance to Meloidogyne spp.and to bipartite and monopartite Begomovirus spp.in wild Solanum (Lycopersicon) accessions［J］. Plant Disease，2010，94(2)：179-185.
［3］Barbary A，Djian-Caporalino C，Palloix A，et al. Host genetic resistance to root-knot nematodes，Meloidogyne spp.，in Solanaceae：from genes to the field［J］. Pest Management Science，2015，71(12)：1591-1598.
［4］Aydnl G，Kurtar E S，Mennan S. Screening of Cucurbita maxima and Cucurbita moschata genotypes for resistance against Meloidogyne arenaria，M. incognita，M. javanica，and M. luci［J］. J Nematol，2019，51(1)：1-10.
［5］芦屹，魏新政，李晶，等. 新疆设施蔬菜根结线虫病调查诊断方法及绿色防控技术［J］. 中国农技推广，2020，36(6)：63-65.
［6］Forghani F，Hajihassani A. Recent advances in the development of environmentally benign treatments to control root-knot nematodes［J］. Frontiers in Plant Science，2020，11：1125.
［7］Eder R，Consoli E，Krauss J，et al. Polysulfides applied as formulated garlic extract to protect tomato plants against the root-knot nematode Meloidogyne incognita［J］. Plants，2021，10(2)：394.
［8］Veremis J C，Roberts P A. Relationships between Meloidogyne incognita resistance genes in Lycopersicon peruvianum differentiated by heat sensitivity and nematode virulence［J］. Theoretical and Applied Genetics，1996，93(5/6)：950-959.
［9］Kaur P，Shukla N，Joshi G，et al. Genome-wide identification and characterization of miRNAome from tomato (Solanum lycopersicum) roots and root-knot nematode (Meloidogyne incognita) during susceptible interaction［J］. PLoS One，2017，12(4)：e0175178.
［10］El-Sappah A H，Islam M M，El-Awady H H，et al. Tomato natural resistance genes in controlling the root-knot nematode［J］. Genes，2019，10(11)：925.
［11］Furumizu C，Sawa S. A rapid method for detection of the root-knot nematode resistance gene，Mi-1.2，in tomato cultivars［J］. Plant Biotechnology，2023，40(1)：105-108.
［12］Caplan J L，Zhu X H，Mamillapalli P，et al. Induced ER chaperones regulate a receptor-like kinase to mediate antiviral innate immune response in plants［J］. Cell Host & Microbe，2009，6(5)：457-469.
［13］Sun T J，Lu Y，Narusaka M，et al. A novel pyrimidin-like plant activator stimulates plant disease resistance and promotes growth［J］. PLoS One，2015，10(4)：e0123227.
［14］Bakker E G，Toomajian C，Kreitman M，et al. A genome-wide survey of R gene polymorphisms in Arabidopsis［J］. The Plant Cell，2006，18(8)：1803-1818.
［15］Xiao K，Zhu H F，Zhu X，et al. Overexpression of PsoRPm3，an NBS-LRR gene isolated from myrobalan plum，confers resistance to Meloidogyne incognita in tobacco［J］. Plant Molecular Biology，2021，107(3)：129-146.
［16］Bozbuga R，Lilley C J，Knox J P，et al. Host-specific signatures of the cell wall changes induced by the plant parasitic nematode，Meloidogyne incognita［J］. Scientific Reports，2018，8：17302.
［17］Nguy[KG-*2]ê~n P V，Bellafiore S，Petitot A S，et al. Meloidogyne incognita-rice (Oryza sativa) interaction：a new model system to study plant-root-knot nematode interactions in monocotyledons［J］. Rice，2014，7(1)：23.
［18］谢政文，王连军，陈锦洋，等. 植物WRKY转录因子及其生物学功能研究进展［J］. 中国农业科技导报，2016，18(3)：46-54.
［19］Russell A R，Ashfield T，Innes R W. Pseudomonas syringae effector AvrPphB suppresses AvrB-induced activation of RPM1 but not AvrRpm1-induced activation［J］. Molecular Plant-Microbe Interactions，2015，28(6)：727-735.
［20］Alam M，Tahir J，Siddiqui A，et al. RIN4 homologs from important crop species differentially regulate the Arabidopsis NB-LRR immune receptor，RPS2［J］. Plant Cell Reports，2021，40(12)：2341-2356.
［21］Ngou B P M，Jones J D G，Ding P T. Plant immune networks［J］. Trends in Plant Science，2022，27(3)：255-273.
［22］Forbes K M，Mappes T，Sironen T，et al. Food limitation constrains host immune responses to nematode infections［J］. Biology Letters，2016，12(9)：20160471.
［23］Kalaiarasan P. Biochemical markers for identification of root knot nematode (Meloidogyne incognita) resistance in tomato［J］. Karnataka Journal of Agricultural Sciences，2009，22(3)：471-475.
［24］Seah S，Williamson V M，Garcia B E，et al. Evaluation of a co-dominant SCAR marker for detection of the Mi-1 locus for resistance to root-knot nematode in tomato germplasm ［J］. Report of the Tomato Cenetics Cooperative，2007，57：37-40.
［25］Sandbrink J M，van Ooijen J W，Purimahua C C，et al. Localization of genes for bacterial canker resistance in Lycopersicon peruvianum using RFLPs［J］. Theoretical and Applied Genetics，1995，90(3/4)：444-450.
［26］Hwang C F，Bhakta A V，Truesdell G M，et al. Evidence for a role of the N terminus and leucine-rich repeat region of the Mi gene product in regulation of localized cell death［J］. The Plant Cell，2000，12(8)：1319-1329.
［27］Yaghoobi J，Kaloshian I，Wen Y，et al. Mapping a new nematode resistance locus in Lycopersicon peruvianum［J］. Theoretical and Applied Genetics，1995，91(3)：457-464.
［28］Hoseinpoor R，Kargar A. Evaluation of the effect powder and aqueous extracts of some plant species on tomato yield and reproduction of Meloidogyne incognita［J］. International Journal of AgriScience，2012，2：964-968.
［29］Yaghoobi J，Yates J L，Williamson V M. Fine mapping of the nematode resistance gene Mi-3 in Solanum peruvianum and construction of a S.lycopersicum DNA contig spanning the locus［J］. Molecular Genetics and Genomics，2005，274(1)：60-69.
［30］Jablonska B，Ammiraju J S S，Bhattarai K K，et al. The Mi-9 gene from Solanum arcanum conferring heat-stable resistance to root-knot nematodes is a homolog of Mi-1［J］. Plant Physiology，2007，143(2)：1044-1054.
［31］Jiang L J，Ling J，Zhao J L，et al. Chromosome-scale genome assembly-assisted identification of Mi-9 gene in Solanum arcanum accession LA2157 conferring heat-stable resistance to Meloidogyne incognita［J］. Plant Biotechnology Journal，2023，21(7)：1496-1509.
［32］Afifah E N，Murti R H，Nuringtyas T R. Metabolomics approach for the analysis of resistance of four tomato genotypes(Solanum lycopersicum L.) to root-knot nematodes(Meloidogyne incognita)［J］. Open Life Sciences，2019，14：141-149.
［33］Eulgem T，Rushton P J，Robatzek S，et al. The WRKY superfamily of plant transcription factors［J］. Trends in Plant Science，2000，5(5)：199-206.
［34］Rushton P J，Somssich I E，Ringler P，et al. WRKY transcription factors［J］. Trends in Plant Science，2010，15(5)：247-258.
［35］任永娟，王东姣，苏亚春，等. 植物WRKY转录因子：结构、分类、进化和功能［J］. 农业生物技术学报，2021，29(1)：105-124.
［36］Bakshi M，Oelmüller R. WRKY transcription factors：Jack of many trades in plants［J］. Plant Signaling & Behavior，2014，9(2)：e27700.
［37］Rinerson C I，Rabara R C，Tripathi P，et al. The evolution of WRKY transcription factors［J］. BMC Plant Biology，2015，15：66.
［38］Chen L G，Song Y，Li S J，et al. The role of WRKY transcription factors in plant abiotic stresses［J］. Biochimica et Biophysica Acta，2012，1819(2)：120-128.
［39］Jiang J J，Ma S H，Ye N H，et al. WRKY transcription factors in plant responses to stresses［J］. Journal of Integrative Plant Biology，2017，59(2)：86-101.
［40］Wani S H，Anand S，Singh B，et al. WRKY transcription factors and plant defense responses：latest discoveries and future prospects［J］. Plant Cell Reports，2021，40(7)：1071-1085.
［41］Kim C Y，Vo K T X，Nguyen C D，et al. Functional analysis of a cold-responsive rice WRKY gene，OsWRKY71［J］. Plant Biotechnology Reports，2016，10(1)：13-23.
［42］Mahiwal S，Pahuja S，Pandey G K. Review：structural-functional relationship of WRKY transcription factors：unfolding the role of WRKY in plants［J］. International Journal of Biological Macromolecules，2024，257(Pt 2)：128769.
［43］Ribeiro D G，Mota A P Z，Santos I R，et al. NBS-LRR-WRKY genes and protease inhibitors (PIs) seem essential for cowpea resistance to root-knot nematode［J］. Journal of Proteomics，2022，261：104575.
［44］Kaliyappan R，Viswanathan S，Suthanthiram B，et al. Evolutionary expansion of WRKY gene family in banana and its expression profile during the infection of root lesion nematode，Pratylenchus coffeae［J］. PLoS One，2016，11(9)：e0162013.
［45］Castaeda N E N，Alves G S C，Almeida R M，et al. Gene expression analysis in Musa acuminata during compatible interactions with Meloidogyne incognita［J］. Annals of Botany，2017，119(5)：915-930.
［46］Hada A，Dutta T K，Singh N，et al. A genome-wide association study in Indian wild rice accessions for resistance to the root-knot nematode Meloidogyne graminicola［J］. PLoS One，2020，15(9)：e0239085.
［47］张雅涵. 黄瓜根结线虫取食位点早期形成相关WRKY基因的分离与分析［D］. 北京：中国农业科学院，2011.
［48］陆秀红，黄金玲，覃丽萍，等. 番茄响应南方根结线虫侵染相关转录因子的初步分析［J］. 华中农业大学学报，2024，43(1)：62-69.
［49］Du C，Jiang J B，Zhang H，et al. Transcriptomic profiling of Solanum peruvianum LA3858 revealed a Mi-3-mediated hypersensitive response to Meloidogyne incognita［J］. BMC Genomics，2020，21(1)：250.
［50］Du C，Shen F Y，Li Y，et al. Effects of salicylic acid，jasmonic acid and reactive oxygen species on the resistance of Solanum peruvianum to Meloidogyne incognita［J］. Scientia Horticulturae，2021，275：109649.
［51］Warmerdam S，Sterken M G，Sukarta O C A，et al. The TIR-NB-LRR pair DSC1 and WRKY19 contributes to basal immunity of Arabidopsis to the root-knot nematode Meloidogyne incognita［J］. BMC Plant Biology，2020，20(1)：73.
［52］郑井元. 辣椒WRKY转录因子CaWRKY6和CaWRKY30基因的克隆、表达及功能分析［D］. 长沙：中南大学，2012.
［53］Zhang M，Zhang H Y，Tan J，et al. Transcriptome analysis of eggplant root in response to root-knot nematode infection［J］. Pathogens，2021，10(4)：470.
［54］Villar-Luna H，Reyes-Trejo B，Gmez-Rodríguez O，et al. Expression of defense genes and accumulation of capsidiol in the compatible interaction Cm334/Nacobbus aberrans and incompatible Cm334/Meloidogyne incognita［J］. Nematropica，2015，45(1)：9-19.
［55］Bhattarai K K，Atamian H S，Kaloshian I，et al. WRKY72-type transcription factors contribute to basal immunity in tomato and Arabidopsis as well as gene-for-gene resistance mediated by the tomato R gene Mi-1［J］. The Plant Journal，2010，63(2)：229-240.
［56］Chinnapandi B，Bucki P，Miyara S B. SlWRKY45，nematode-responsive tomato WRKY gene，enhances susceptibility to the root knot nematode；M.javanica infection［J］. Plant Signaling & Behavior，2017，12(12)：e1356530.
［57］Huang H，Zhao W C，Qiao H，et al. SlWRKY45 interacts with jasmonate-ZIM domain proteins to negatively regulate defense against the root-knot nematode Meloidogyne incognita in tomato［J］. Horticulture Research，2022，9：uhac197.
［58］Chinnapandi B，Bucki P，Fitoussi N，et al. Tomato SlWRKY3 acts as a positive regulator for resistance against the root-knot nematode Meloidogyne javanica by activating lipids and hormone-mediated defense-signaling pathways［J］. Plant Signaling & Behavior，2019，14(6)：1601951.
［59］Nie W D，Liu L L，Chen Y X，et al. Identification of the regulatory role of SlWRKYs in tomato defense against Meloidogyne incognita［J］. Plants，2023，12(13)：2416.
［60］Kiewnick S，Dessimoz M，Franck L. Effects of the Mi-1 and the N root-knot nematode-resistance gene on infection and reproduction of Meloidogyne enterolobii on tomato and pepper cultivars［J］. Journal of Nematology，2009，41(2)：134-139.
［61］Brito J A，Stanley J D，Mendes M L，et al. Host status of selected cultivated plants to Meloidogyne mayaguensis in Florida［J］. Nematropica，2007，37(1)：65-72.
［62］Wang Y，Bao Z L，Zhu Y，et al. Analysis of temperature modulation of plant defense against biotrophic microbes［J］. Molecular Plant-Microbe Interactions，2009，22(5)：498-506.
［63］Iberkleid I，Ozalvo R，Feldman L，et al. Responses of tomato genotypes to avirulent and Mi-virulent Meloidogyne javanica isolates occurring in Israel［J］. Phytopathology，2014，104(5)：484-496.
［64］de Brida A L，Correiaé C S D S，Castro B M C E，et al. Oat，wheat，and Sorghum genotype reactions to Meloidogyne incognita and Meloidogyne javanica［J］. Journal of Nematology，2017，49(4)：386-389.

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备注/Memo

备注/Memo:
收稿日期：2024-04-08
基金项目：国家自然科学基金青年科学基金(编号：32302652)；新疆维吾尔自治区自然科学基金青年科学基金(编号：2022D01B95)。
作者简介：陈银霞(1996—)，女，河南淮阳人，硕士研究生，研究方向为番茄分子遗传育种。E-mail：cyx61222@163.com。
通信作者：杜崇，博士，硕士生导师，主要从事番茄分子遗传育种研究。E-mail：godv2018@163.com。
更新日期/Last Update: 2025-02-20