[1]闫博巍,常传义,隋月,等.工业大麻CsDGAT1的生物信息学及表达模式分析[J].江苏农业科学,2025,53(19):16-26.
 Yan Bowei,et al.Bioinformatics and expression pattern analysis of CsDGAT1 gene in hemp[J].Jiangsu Agricultural Sciences,2025,53(19):16-26.
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工业大麻CsDGAT1的生物信息学及表达模式分析()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第53卷
期数:
2025年第19期
页码:
16-26
栏目:
生物技术
出版日期:
2025-10-05

文章信息/Info

Title:
Bioinformatics and expression pattern analysis of CsDGAT1 gene in hemp
作者:
闫博巍1常传义2隋月1郑楠1房郁妍1张元野1张明1张利国1
1.黑龙江省农业科学院经济作物研究所,黑龙江哈尔滨 150086; 2.哈尔滨市农业科学院,黑龙江哈尔滨 150029
Author(s):
Yan Boweiet al
关键词:
工业大麻CsDGAT1蛋白质生物信息学实时定量PCR技术低温胁迫
Keywords:
-
分类号:
S184;S188;S563.301
DOI:
-
文献标志码:
A
摘要:
通过生物信息学分析软件及相关数据库,对CsDGAT1基因进行全基因组鉴定及生物信息学分析;构建CsDGAT1与GFP融合表达载体并瞬时转化烟草叶片,以确定其亚细胞定位;同时,采用实时荧光定量PCR技术检测CsDGAT1的组织特异性表达及其在低温胁迫下的表达模式。结果表明,CsDGAT1基因编码区全长1 644 bp,编码547个氨基酸,蛋白质预测分子量为 62.4 ku,不稳定系数为45.08,属不稳定蛋白质;理论等电点为8.7,属于碱性蛋白质。亚细胞定位结果表明,CsDGAT1定位于内质网。该蛋白质含有1个MBOAT结构域,以及酰基辅酶A结合域、SnRK1靶位点、DAG结合基序等保守功能区,且与双子叶植物的DGAT蛋白质亲缘关系更密切。CsDGAT1基因启动子区域包含丰富的胁迫响应元件、光响应元件、植物激素响应元件及植物发育响应元件。组织表达分析显示,该基因在雌花和种子中的表达量高于其他组织。低温胁迫条件下,CsDGAT1基因在处理中后期的表达量显著上调。综上,工业大麻中仅存在1个CsDGAT1成员,其生物信息学特征及表达模式表明该基因可能参与低温胁迫应答,该结果为工业大麻品质改良与耐低温新品种的选育提供了基因资源。
Abstract:
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备注/Memo

备注/Memo:
收稿日期:2024-09-03
基金项目:黑龙江省农业科技创新跨越工程重点项目(编号:GX23GG04);中国博士后科学基金第14批特别资助(编号:2021T140187)。
作者简介:闫博巍(1990—),女,黑龙江哈尔滨人,博士,助理研究员,主要从事工业大麻逆境响应机理研究。E-mail:yanbowei21@outlook.com。
通信作者:张利国,博士,研究员,主要从事工业大麻育种及产业化应用。E-mail:hljzhangliguo@16.com。
更新日期/Last Update: 2025-10-05