[1]李敏敏,何小春,张继雨,等.基于全基因组重测序开发鲜食大豆通酥1号InDel分子鉴别标记[J].江苏农业科学,2025,53(22):73-80.
　Li Minmin,et al.Development of InDel molecular identification markers for vegetable soybean Tongsu No.1 based on whole genome resequencing[J].Jiangsu Agricultural Sciences,2025,53(22):73-80.
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基于全基因组重测序开发鲜食大豆通酥1号InDel分子鉴别标记()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第53卷
期数:
2025年第22期
页码:
73-80
栏目:
生物技术
出版日期:
2025-11-20

文章信息/Info

Title:
Development of InDel molecular identification markers for vegetable soybean Tongsu No.1 based on whole genome resequencing
作者:
李敏敏1; 何小春1; 张继雨2; 朱治佳3; 邱牧1; 赵杨1; 李磊4; 张正睿1
1.聊城市农业科学院，山东聊城 252000； 2.菏泽市农业科学院，山东菏泽 274000；3.黑龙江省农业科学院齐齐哈尔分院，黑龙江齐齐哈尔 161000； 4.聊城市财金能源发展有限公司，山东聊城 252000
Author(s):
Li Minmin; et al
关键词:
全基因组; 重测序; 鲜食大豆; InDel分子标记
Keywords:
-
分类号:
S188；S565.101
DOI:
-
文献标志码:
A
摘要:
为从全基因组序列水平解析鲜食大豆品种通酥1号的遗传构成，采用Illumina公司的NovaSeq测序平台进行全基因组测序，平均覆盖深度为27.08×，系统分析通酥1号单核苷酸多态性(SNP)、插入/缺失(InDel)、拷贝数变异(CNV)和染色体结构变异(SV)等序列变异。结果显示，在鲜食大豆品种通酥1号中共检测到1 744 120个SNP，其中73.66%的SNP位于基因间区，而在位于外显子区域的4.08%变异中，53.83%为非同义突变，可能导致2 883个基因蛋白质功能改变。鉴定出232 213个InDel变异，其中短片段(1~4 bp)变异占比明显高于长片段(≥5 bp)，可能是由于短片段变异对基因组稳定性的破坏较小，被自然选择容忍。在外显子区域的2 986个InDel变异中，约1.28%(39个)引起Glyma.02g123100等22个基因提前终止密码子，可能显著影响基因功能；约0.13%(5个)导致Glyma.15g187700等5个基因终止密码子丢失，可能通过延长蛋白翻译产生功能获得性突变。检测到8 732个CNV和5 749个SV，分别导致5 982、24 594个基因发生变异。最后基于14个InDel变异开发鉴别标记，其中标记InDel_1、InDel_8、InDel_12分别导致Glyma.02g290500、Glyma.10g103100、Glyma.16g194600基因发生序列变异，可能通过调节风味、影响细胞壁结构与质地等途径影响大豆鲜食特性。这些标记的进一步转化将提升育种效率，加速优质鲜食大豆品种的选育进程。
Abstract:
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备注/Memo

备注/Memo:
收稿日期：2025-05-07
基金项目：聊城市重点研发计划政策引导类项目(编号：2024YD91)。
作者简介：李敏敏(1991—)，女，山东聊城人，博士，高级农艺师，主要从事大豆遗传育种与栽培研究。E-mail：liminmin527@163.com。
更新日期/Last Update: 2025-11-20