[1]唐跃辉,段雨彤,邓雨涵,等.麻风树JcHDZ12基因克隆与干旱胁迫响应功能分析[J].江苏农业科学,2026,54(8):38-43.
　Tang Yuehui,et al.Cloning and drought stress response function analysis of JcHDZ12 gene in Jatropha curcas[J].Jiangsu Agricultural Sciences,2026,54(8):38-43.
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麻风树JcHDZ12基因克隆与干旱胁迫响应功能分析()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第54卷
期数:
2026年第8期
页码:
38-43
栏目:
耐干旱基因鉴定
出版日期:
2026-04-20

文章信息/Info

Title:
Cloning and drought stress response function analysis of JcHDZ12 gene in Jatropha curcas
作者:
唐跃辉1; 段雨彤2; 邓雨涵1; 程子诺1; 许嘉文1; 王圆圆1; 陈芯茹1; 李雪纯1; 包欣欣3
1.周口师范学院生命科学与农学学院，河南周口 466001； 2.周口师范学院博农科技学院，河南周口 466001； 3.周口师范学院新闻与传媒学院，河南周口 466001
Author(s):
Tang Yuehui; et al
关键词:
麻风树; HD-Zip转录因子; JcHDZ12; 干旱胁迫
Keywords:
-
分类号:
S188
DOI:
-
文献标志码:
A
摘要:
探究麻风树HD-Zip转录因子JcHDZ12在植物响应干旱胁迫中的调控功能，为麻风树耐旱分子机制研究提供基础。以根cDNA为模版，采用RT-PCR技术克隆JcHDZ12基因，借助DNAMAN软件分析其氨基酸序列特征，利用ExPASy 网站对JcHDZ12的理化特性进行分析，通过qRT-PCR技术分析其表达模式，利用拟南芥原生质体亚细胞定位技术明确其定位情况，并通过农杆菌介导的花粉浸染法构建 JcHDZ12转基因拟南芥植株，利用聚乙二醇模拟干旱法分析JcHDZ12转基因植株的抗旱性。结果表明，JcHDZ12基因全长1 531 bp，包含801 bp的开放阅读框，编码266个氨基酸；JcHDZ12在根中的表达量最高，且干旱胁迫抑制该基因的表达；JcHDZ12基因编码一个核定位蛋白；过表达JcHDZ12增加了转基因拟南芥对干旱胁迫的敏感性，且干旱胁迫下转基因植株脯氨酸含量、超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性极显著低于野生型(P<0.01)，相对电导率极显著高于野生型(P<0.01)；干旱胁迫下，非生物胁迫相关基因AtP5CS和AtAPX2的表达量极显著低于野生型，而正常条件下这些基因在转基因植株和野生型中的表达没有显著差异。本研究结果可为将来深入研究JcHDZ12基因在麻风树响应干旱胁迫中的功能提供理论依据。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2025-10-15
基金项目：河南省科技研发计划联合基金(编号：245101610087)；河南省科技攻关项目(编号：252102110274)。
作者简介：唐跃辉(1985—)，男，河南许昌人，博士，副教授，主要从事植物逆境分子生物学研究。E-mail：yhtang2005@163.com。
更新日期/Last Update: 2026-04-20