[1]陈为,张琳,张卫凯.麻风树Trihelix基因家族的鉴定及功能分析[J].江苏农业科学,2026,54(8):70-79.
　Chen Wei,et al.Identification and function analysis of Trihelix gene family in Jatropha curcas L.[J].Jiangsu Agricultural Sciences,2026,54(8):70-79.
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麻风树Trihelix基因家族的鉴定及功能分析()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第54卷
期数:
2026年第8期
页码:
70-79
栏目:
耐干旱基因鉴定
出版日期:
2026-04-20

文章信息/Info

Title:
Identification and function analysis of Trihelix gene family in Jatropha curcas L.
作者:
陈为1; 张琳2; 张卫凯1
1.洛阳职业技术学院河南省王云龙中原学者工作站，河南洛阳 471000； 2.洛阳理工学院生命科学与健康工程学院，河南洛阳 471000
Author(s):
Chen Wei; et al
关键词:
Trihelix基因家族; 麻风树; 盐胁迫; 生物信息学分析; 干旱胁迫; 表达模式
Keywords:
-
分类号:
S188
DOI:
-
文献标志码:
A
摘要:
Trihelix转录因子在植物生长发育及非生物胁迫响应中具有关键作用，目前已在拟南芥、水稻等模式植物中开展相关研究，但在麻风树中尚未见报道。为鉴定麻风树Trihelix基因家族成员，解析其生物信息学特征及表达模式，为抗逆育种提供候选基因。研究基于麻风树基因组数据，通过HMM搜索、系统发育分析等生物信息学方法，结合表达谱数据探究基因在不同组织及盐、干旱胁迫下的表达情况。结果表明，共鉴定出32个JcGT基因，可分为5个亚家族及2个孤儿基因；其中28个基因分布于9个连锁群，4个位于未定位的scaffold上，且仅存在3个片段复制事件。多数基因表达较为稳定，JcGT27在花芽中特异性表达；盐胁迫下JcGT-6、JcGT-9在根中显著上调表达，干旱胁迫下JcGT-18、JcGT-29等在根中上调表达，而JcGT-16、JcGT-26对2种胁迫均有响应。本研究首次完成了麻风树Trihelix基因家族的全基因组分析，证实片段复制是其扩张的主要驱动力，筛选出的关键候选基因可为后续功能研究及抗逆作物培育提供理论依据。
Abstract:
-

参考文献/References:

［1］Nagano Y. Several features of the GT-factor trihelix domain resemble those of the Myb DNA-binding domain［J］. Plant Physiology，2000，124(2)：491-494.
［2］Fang Y J，Xie K B，Hou X，et al. Systematic analysis of GT factor family of rice reveals a novel subfamily involved in stress responses［J］. Molecular Genetics and Genomics，2010，283(2)：157-169.
［3］Kaplan-Levy R N，Brewer P B，Quon T，et al. The trihelix family of transcription factors-light，stress and development［J］. Trends in Plant Science，2012，17(3)：163-171.
［4］Yu C Y，Cai X F，Ye Z B，et al. Genome-wide identification and expression profiling analysis of trihelix gene family in tomato［J］. Biochemical and Biophysical Research Communications，2015，468(4)：653-659.
［5］Li J M，Zhang M H，Sun J，et al. Genome-wide characterization and identification of trihelix transcription factor and expression profiling in response to abiotic stresses in rice (Oryza sativa L.)［J］. International Journal of Molecular Sciences，2019，20(2)：251.
［6］Yasmeen E，Riaz M，Sultan S，et al. Genome-wide analysis of trihelix transcription factor gene family in Arabidopsis thaliana［J］. Pakistan Journal of Agricultural Sciences，2016，53(2)：439-448.
［7］Liu W，Zhang Y W，Li W，et al. Genome-wide characterization and expression analysis of soybean trihelix gene family［J］. PeerJ，2020，8：e8753.
［8］Xiao J，Hu R，Gu T，et al. Genome-wide identification and expression profiling of trihelix gene family under abiotic stresses in wheat［J］. BMC Genomics，2019，20(1)：287.
［9］OBrien M，Kaplan-Levy R N，Quon T，et al. PETAL LOSS，a trihelix transcription factor that represses growth in Arabidopsis thaliana，binds the energy-sensing SnRK1 kinase AKIN10［J］. Journal of Experimental Botany，2015，66(9)：2475-2485.
［10］Shibata M，Breuer C，Kawamura A，et al. gtl1 and df1 regulate root hair growth through transcriptional repression of root hair defective 6-like 4 in Arabidopsis［J］. Development，2018，145(3)：dev159707.
［11］Wan C M，Li C M，Ma X Z，et al. GRY79 encoding a putative metallo-β-lactamase-trihelix Chimera is involved in chloroplast development at early seedling stage of rice［J］. Plant Cell Reports，2015，34(8)：1353-1363.
［12］Li P，Li Z X，Xie G N，et al. Trihelix transcription factor ZmThx20 is required for kernel development in maize［J］. International Journal of Molecular Sciences，2021，22(22)：12137.
［13］Yang L L，Qi S L，Touqeer A，et al. SlGT11 controls floral organ patterning and floral determinacy in tomato［J］. BMC Plant Biology，2020，20(1)：562.
［14］Li B，Jiang S，Yu X，et al. Phosphorylation of trihelix transcriptional repressor ASR3 by MAP KINASE4 negatively regulates Arabidopsis immunity［J］. The Plant Cell，2015，27(3)：839-856.
［15］Vlz R，Kim S K，Mi J N，et al. The Trihelix transcription factor GT2-like 1 (GTL1) promotes salicylic acid metabolism，and regulates bacterial-triggered immunity［J］. PLoS Genetics，2018，14(10)：e1007708.
［16］Zhang Q Q，Zhong T，E L Z，et al. GT factor ZmGT-3b is associated with regulation of photosynthesis and defense response to Fusarium graminearum infection in maize seedling［J］. Frontiers in Plant Science，2021，12：724133.
［17］Xie Z M，Zou H F，Lei G，et al. Soybean Trihelix transcription factors GmGT-2A and GmGT-2B improve plant tolerance to abiotic stresses in transgenic Arabidopsis［J］. PLoS One，2009，4(9)：e6898.
［18］Yu C Y，Song L L，Song J W，et al. ShCIGT，a Trihelix family gene，mediates cold and drought tolerance by interacting with SnRK1 in tomato［J］. Plant Science，2018，270：140-149.
［19］Magwanga R O，Kirungu J N，Lu P，et al. Genome wide identification of the trihelix transcription factors and overexpression of Gh_A05G2067 (GT-2)，a novel gene contributing to increased drought and salt stresses tolerance in cotton［J］. Physiologia Plantarum，2019，167(3)：447-464.
［20］Zheng X，Liu H P，Ji H T，et al. The wheat GT factor TaGT2L1D negatively regulates drought tolerance and plant development［J］. Scientific Reports，2016，6：27042.
［21］Weng H，Yoo C Y，Gosney M J，et al. Poplar GTL1 is a Ca2+/calmodulin-binding transcription factor that functions in plant water use efficiency and drought tolerance［J］. PLoS One，2012，7(3)：e32925.
［22］Fu M J，Li F F，Zhou S G，et al. Trihelix transcription factor SlGT31 regulates fruit ripening mediated by ethylene in tomato［J］. Journal of Experimental Botany，2023，74(18)：5709-5721.
［23］Li F F，Chen G P，Xie Q L，et al. Down-regulation of SlGT-26 gene confers dwarf plants and enhances drought and salt stress resistance in tomato［J］. Plant Physiology and Biochemistry，2023，203：108053.
［24］Lv H M，Wang X W，Dong X N，et al. CRISPR/Cas9 edited SlGT30 improved both drought resistance and fruit yield through endoreduplication［J］. Plant，Cell & Environment，2025，48(4)：2581-2595.
［25］Wang Y，Tang M，Zhang Y，et al. Coordinated regulation of plant defense and autoimmunity by paired trihelix transcription factors ASR3/AITF1 in Arabidopsis［J］. New Phytologist，2023，237(3)：914-929.
［26］Zheng X Z，Jian Y F，Long Q，et al. SlASR3 mediates crosstalk between auxin and jasmonic acid signaling to regulate trichome formation in tomato［J］. The Plant Journal，2025，121(4)：e70053.
［27］Zhao J L，Huang K W，Liu R，et al. The root-knot nematode effector Mi2G02 hijacks a host plant trihelix transcription factor to promote nematode parasitism［J］. Plant Communications，2024，5(2)：100723.
［28］Wu P Z，Zhou C P，Cheng S F，et al. Integrated genome sequence and linkage map of physic nut (Jatropha curcas L.)，a biodiesel plant［J］. The Plant Journal，2015，81(5)：810-821.
［29］Qin Y，Ma X，Yu G H，et al. Evolutionary history of trihelix family and their functional diversification［J］. DNA Research，2014，21(5)：499-510.
［30］Ma Z T，Liu M Y，Sun W J，et al. Genome-wide identification and expression analysis of the trihelix transcription factor family in Tartary buckwheat (Fagopyrum tataricum)［J］. BMC Plant Biology，2019，19(1)：344.
［31］Riechmann J L，Heard J，Martin G，et al. Arabidopsis transcription factors：genome-wide comparative analysis among eukaryotes［J］. Science，2000，290(5499)：2105-2110.
［32］Li K Y，Fan Y，Zhou G Y，et al. Genome-wide identification，phylogenetic analysis，and expression profiles of trihelix transcription factor family genes in quinoa (Chenopodium quinoa Willd.) under abiotic stress conditions［J］. BMC Genomics，2022，23(1)：499.
［33］Song A P，Wu D，Fan Q Q，et al. Transcriptome-wide identification and expression profiling analysis of Chrysanthemum Trihelix transcription factors［J］. International Journal of Molecular Sciences，2016，17(2)：198.
［34］Wang Z C，Liu Q G，Wang H Z，et al. Comprehensive analysis of trihelix genes and their expression under biotic and abiotic stresses in Populus trichocarpa［J］. Scientific Reports，2016，6：36274.
［35］Li K Y，Duan L L，Zhang Y B，et al. Genome-wide identification and expression profile analysis of trihelix transcription factor family genes in response to abiotic stress in sorghum ［Sorghum bicolor(L.) Moench］［J］. BMC Genomics，2021，22(1)：738.
［36］Shabalina S A，Ogurtsov A Y，Spiridonov A N，et al. Distinct patterns of expression and evolution of intronless and intron-containing mammalian genes［J］. Molecular Biology and Evolution，2010，27(8)：1745-1749.
［37］Wu T，Yang Q H，Zhou R，et al. Large-scale analysis of trihelix transcription factors reveals their expansion and evolutionary footprint in plants［J］. Physiologia Plantarum，2023，175(5)：e14039.
［38］Ibarra E，Reynoso M A. Plant trihelix transcription factors and their functions in development and stress［J］. Journal of Experimental Botany，2025，76(17)：4849-4868.
［39］Brewer P B，Howles P A，Dorian K，et al. PETAL LOSS a trihelix transcription factor gene，regulates perianth architecture in the Arabidopsis flower［J］. Development，2004，131(16)：4035-4045.
［40］Wang X H，Li Q T，Chen H W，et al. Trihelix transcription factor GT-4 mediates salt tolerance via interaction with TEM2 in Arabidopsis［J］. BMC Plant Biology，2014，14(1)：339.
［41］Lynch J. Root architecture and plant productivity［J］. Plant Physiology，1995，109(1)：7-13.

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备注/Memo

备注/Memo:
收稿日期：2025-10-31
基金项目：河南省科技攻关项目(编号：262102111058)。
作者简介：陈为(1981— )，男，湖北赤壁人，博士，讲师，主要从事基因挖掘与功能研究。E-mai：202332007@lypt.edu.cn。
更新日期/Last Update: 2026-04-20