[1]吴文博,张博,王志淇,等.马铃薯GDSL95基因的克隆及表达模式分析[J].江苏农业科学,2026,54(9):45-56.
　Wu Wenbo,et al.Cloning and expression pattern analysis of GDSL95 gene in potato[J].Jiangsu Agricultural Sciences,2026,54(9):45-56.
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马铃薯GDSL95基因的克隆及表达模式分析()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第54卷
期数:
2026年第9期
页码:
45-56
栏目:
生物技术
出版日期:
2026-05-05

文章信息/Info

Title:
Cloning and expression pattern analysis of GDSL95 gene in potato
作者:
吴文博1; 张博1; 王志淇1; 刘涛1; 马凯1; 杨中敏1; 2
1.新疆农业大学园艺学院，新疆乌鲁木齐 830052； 2.新疆农业大学园艺学博士后流动站，新疆乌鲁木齐 830052
Author(s):
Wu Wenbo; et al
关键词:
马铃薯; GDSL基因家族; 生物信息学; 激素; 表达模式分析
Keywords:
-
分类号:
S188；S532.01
DOI:
-
文献标志码:
A
摘要:
GDSL脂肪酶是一类多功能水解酶，具有广泛的底物特异性和重要的生物学功能。植物GDSL脂肪酶家族包含较多的成员，但在马铃薯中报道较少。基于马铃薯DM v6.1基因组鉴定GDSL基因家族成员并对其进行生物信息学分析，挑选出GDSL95基因进行克隆并进行不同组织部位及不同激素处理情况下的表达量分析。结果表明，在马铃薯中共鉴定到96个GDSL家族基因，亚细胞定位预测StGDSL蛋白大部分存在于细胞外基质、叶绿体和细胞质基质中；共线性分析发现，StGDSL家族在马铃薯中存在15对共线性基因，与番茄和拟南芥分别存在56对和44对共线性基因。StGDSL95 ORF全长为1 110 bp，编码369个氨基酸；启动子顺式作用元件分析发现，StGDSL95基因启动子有大量光响应、激素应答等元件；互作蛋白预测发现，StGDSL95与细胞色素P450(如CYP703A2)和磷脂酶PLIP3相关；RT-qPCR分析显示，StGDSL95基因在根、匍匐茎、花药中表达量较高，在低表达的茎、叶中也积极响应赤霉素的诱导。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2025-04-28
基金项目：新疆维吾尔自治区自然科学基金(编号：2022D01B96)；国家自然科学基金(编号：32260765)。
作者简介：吴文博(1999—)，男，宁夏吴忠人，硕士研究生，研究方向为马铃薯分子遗传育种。E-mail：173522984@qq.com。
通信作者：杨中敏，博士，副教授，主要从事马铃薯分子遗传育种研究。E-mail：yangzhongmin161220@126.com。
更新日期/Last Update: 2026-05-05