|本期目录/Table of Contents|

[1]杨正久,钱静,张连英,等.野油菜黄单胞菌葡萄糖激酶基因的克隆与表达[J].江苏农业科学,2013,41(06):14-18.
 Yang Zhengjiu,et al.Cloning and expression of glucokinase gene of Xanthomonas campestris pv. campestris[J].Jiangsu Agricultural Sciences,2013,41(06):14-18.
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第41卷
期数:
2013年06期
页码:
14-18
栏目:
生物技术
出版日期:
2013-06-25

文章信息/Info

Title:
Cloning and expression of glucokinase gene of Xanthomonas campestris pv. campestris
作者:
杨正久1 钱静1 张连英1 董红梅1 陆光涛2
1.遵义医药高等专科学校医学技术系,贵州遵义 563000; 2.广西大学生命科学与技术学院,广西南宁 530005
Author(s):
Yang Zhengjiuet al
关键词:
野油菜黄单胞菌葡萄糖激酶克隆表达
Keywords:
-
分类号:
Q75
DOI:
-
文献标志码:
A
摘要:
葡萄糖激酶催化葡萄糖磷酸化生成葡萄糖-6-磷酸,是糖酵解的第一步反应,在野油菜黄单胞菌(Xcc)利用碳源代谢及其致病性中起重要作用。为了初步鉴定Xcc葡萄糖激酶基因功能,为研究其利用碳源相关代谢及致病性提供理论依据,以pQE30为表达载体,采用常规PCR技术对野油菜黄单胞菌Xcc8004基因组上注释为葡萄糖激酶的3个基因(XC_1166、XC_1223和XC_1976)进行了克隆与表达,该3个基因在大肠杆菌JM109中有较高表达量,经Ni2+金属层析柱纯化后分别得到带有3个His的融合蛋白XC_1166-His6、XC_1223-His6和XC_1976-His6,分析表明仅XC_1976的表达产物具有葡萄糖激酶活性,为Xcc8004葡萄糖激酶基因,经测定XC_1976-His6的最适反应温度为45 ℃,最适pH值为7.1,最适反应条件下比活力为(380.6±1.123) U/mg。
Abstract:
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参考文献/References:

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相似文献/References:

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备注/Memo

备注/Memo:
收稿日期:2013-01-11
基金项目:国家自然科学基金重点项目(编号:30130010);国家自然科学基金面上项目(编号:30470944)。
作者简介:杨正久(1975—),男,苗族,湖南泸溪人,硕士,讲师,主要从事生物化学的教学和研究,E -mail:yang282@163. com。
通信作者:陆光涛,博士,主要从事微生物多糖的生物合成机理、植物病原菌致病的分子机理。E-mail:lugt@gxu.edu.cn。
更新日期/Last Update: 2013-06-25