|本期目录/Table of Contents|

[1]汪牧西,刘朝莹,宁德刚.集胞藻PCC6803染色体上抗毒素-毒素基因ssl2749-sll1411的转录调控[J].江苏农业科学,2013,41(06):18-20.
　Wang Muxi,et al.Transcriptional regulation of antitoxin-toxin genes ssl2749-sll1411 on chromosome of Synechocystis sp. PCC6803[J].Jiangsu Agricultural Sciences,2013,41(06):18-20.
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集胞藻PCC6803染色体上抗毒素-毒素基因ssl2749-sll1411的转录调控(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第41卷
期数:
2013年06期
页码:
18-20
栏目:
生物技术
出版日期:
2013-06-25

文章信息/Info

Title:
Transcriptional regulation of antitoxin-toxin genes ssl2749-sll1411 on chromosome of Synechocystis sp. PCC6803
作者:
汪牧西;  刘朝莹;  宁德刚
江苏大学环境与安全工程学院，江苏镇江 212013
Author(s):
Wang Muxi; et al
关键词:
集胞藻; 染色体; ssl2749-sll1411基因; 转录调控
Keywords:
-
分类号:
Q75
DOI:
-
文献标志码:
A
摘要:
细菌染色体上的mnt/hepn操纵子构成毒素－抗毒素系统(TA，toxin-antitoxin system)新家族，但该家族成员的生化及遗传特征有待阐明。为了证明集胞藻PCC6803染色体上基因ssl2749/sll1411的转录调控作用，以无启动子的β-半乳糖苷酶基因(lacZ)为报告基因，构建了lacZ转录融合重组质粒，通过测定含重组质粒的大肠杆菌细胞的β-半乳糖苷酶活性，确定ssl2749/sll1411编码产物对其启动子活性的调控作用。结果表明，蛋白Ssl2749使β-半乳糖苷酶活性显著增加，提示Ssl2749可激活PTA启动子转录活性，蛋白Sll1411抑制Ssl2749的转录激活作用。因此，Ssl2749可能与PTA中的调控序列结合调控ssl2749/sll1411操纵子的转录，Sll1411对Ssl2749转录激活活性的抑制作用提示二者之间存在相互作用。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2012-11-29
基金项目：国家自然科学基金(编号：30771176)。
作者简介：汪牧西，男，湖南湘潭人，硕士研究生，主要从事环境微生物(蓝藻)方面的研究。E-mail：wang_mu_xi@126.com。
更新日期/Last Update: 2013-06-25