|本期目录/Table of Contents|

[1]王琳琳,陈长春,徐婷婷,等.猪圆环病毒 Ⅱ 型Cap蛋白单克隆抗体的制备及初步应用[J].江苏农业科学,2013,41(07):30-31.
 Wang Linlin,et al.Preparation and preliminary application of monoclonal antibodies against capsid protein of porcine circovirus type 2[J].Jiangsu Agricultural Sciences,2013,41(07):30-31.
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猪圆环病毒 Ⅱ 型Cap蛋白单克隆抗体的制备及初步应用(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第41卷
期数:
2013年07期
页码:
30-31
栏目:
生物技术
出版日期:
2013-07-25

文章信息/Info

Title:
Preparation and preliminary application of monoclonal antibodies against capsid protein of porcine circovirus type 2
作者:
王琳琳 陈长春 徐婷婷 戴丽红
江苏农牧科技职业学院,江苏泰州 225300
Author(s):
Wang Linlinet al
关键词:
猪圆环病毒Ⅱ型Cap蛋白单克隆抗体
Keywords:
-
分类号:
S852.65+1
DOI:
-
文献标志码:
A
摘要:
利用PCV2病毒免疫8周龄BALB/c雌性小鼠,取其脾细胞与SP2/0骨髓瘤细胞融合,原核表达的PCV2病毒Cap蛋白作为检测抗原进行ELISA检测,获得了1株PCV2单抗,命名为2E9。用PCV2全病毒免疫小鼠,能获得特异性针对PCV2病毒的单克隆抗体。为建立特异性强、敏感性高、简便快速的PCV2检测方法打下了基础。
Abstract:
-

参考文献/References:

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[2]Allan G M,McNeilly F,Meehan B M,et al.Isolation and characterization of circoviruses from pigs with wasting syndromes in Spain,Denmark and Northern Ireland[J]. Vet Microbiol,1999,66(2):115-123.
[3]Hamel A L,Lin L L,Sachvie C,et al. PCR detection and characterization of type-2 porcine circovirus[J]. Can J Vet Res,2000,64(1):44-52.
[4]Kennedy S,Segales J,Rovira A,et al.Absence of evidence of porcine circovirus infection in piglets with congenital tremors[J]. J Vet Diagn Invest,2003,15(2):151-156.
[5]McNcilly F,McNair I. Isolation and chanracterization of porcine circovrus 2 from cases of sow abortion and porcine dermatitis and nephropathy syndrome[J]. Arch Virol,2001,146(40):83-84.
[6]Mankertz A,Hillenbrand B. Analysis of transcription of porcine circovirus type 1[J]. J Gen virol,2002,83:2743-2751.
[7]Nawagitgul P,Morozov I,Bolin S,et al.Open reading frame 2 of porcine circovirus type 2 encodes a major capsid protein[J]. J Gen Virol,2000,81:2281-2287.
[8]Pogranichnyy R M,Yoon K J,Harms P A,et al.Characterization of immune response of young pigs to porcine circovirus type 2 infection[J]. Viral Immunol,2000,13:143-153.
[9]Liu Q,Tikoo S K.,Babiuk L A.Nuclear localization of the ORF2 protein encoded by porcine circovirus type 2[J]. Virology,2001,285:91-99.
[10]Lekcharoensuk P,Morozov I,Paul P S,et al. Pitope mapping of the major capsid protein of type 2 porcine circovirus(PCV2)by using chimeric PCV1 and PCV2[J]. J Virol,2004,78(15):8135-8145.
[11]Grierson S S,King D P,Wellenberg G J,et al.Genome sequence analysis of 10 Dutch porcine circovirus type 2(PCV-2) isolates from a PMWS case-control study[J]. Res Vet Sci,2004,77(3):265-268.
[12] Segales J,Olvera A,Grau-Roma L,et al.PCV-2 geno-type definition and nomenclature[J]. Vet Rec,2008,162(26):867-868.

相似文献/References:

[1]陶宏卫,郭广富,曹军平,等.猪圆环病毒Ⅱ型盐城株的全基因组克隆与序列分析[J].江苏农业科学,2018,46(16):39.
 Tao Hongwei,et al.Cloning and sequence analysis of Yancheng isolates of porcine circovirus type 2[J].Jiangsu Agricultural Sciences,2018,46(07):39.
[2]丁丽军,陈长春,夏前贤.环病毒Ⅱ型Cap蛋白单克隆抗体的制备及初步应用[J].江苏农业科学,2013,41(11):44.
 Ding Lijun,et al.Preparation and preliminary application of monoclonal antibodies against capsid protein Porcine circovirus type 2[J].Jiangsu Agricultural Sciences,2013,41(07):44.

备注/Memo

备注/Memo:
收稿日期:2013-06-14
作者简介:王琳琳(1980—),女,江苏泰州人,助教,从事动物医学研究。E-mail:54831842@qq.com。
更新日期/Last Update: 2013-07-25