|本期目录/Table of Contents|

[1]李珺,马力通.马铃薯淀粉体表达载体的构建及其转基因植物的培养[J].江苏农业科学,2013,41(08):22-24.
 Li Jun,et al.Construction of potato amyloplast expression vector and breeding of its genetically modified plants[J].Jiangsu Agricultural Sciences,2013,41(08):22-24.
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马铃薯淀粉体表达载体的构建及其转基因植物的培养(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第41卷
期数:
2013年08期
页码:
22-24
栏目:
生物技术
出版日期:
2013-08-25

文章信息/Info

Title:
Construction of potato amyloplast expression vector and breeding of its genetically modified plants
作者:
李珺 马力通
内蒙古科技大学生物工程与技术研究所,内蒙古包头 014010
Author(s):
Li Junet al
关键词:
GBSSⅠ基因启动子马铃薯淀粉体生物反应器
Keywords:
-
分类号:
Q943.2
DOI:
-
文献标志码:
A
摘要:
以pCAMBIA1305-1载体为基础,构建融合了淀粉体转运肽GB77及淀粉粒结合肽GB20编码序列的马铃薯淀粉体定向表达载体pCAMBIA1305-GB77-GB20,并通过马铃薯块茎专一性GBSSⅠ基因启动子启动其表达。将构建的载体经农杆菌介导转化蜡质马铃薯突变植株,PCR证实了融合基因已经整合到马铃薯基因组中。研究为探讨马铃薯淀粉体定向表达外源蛋白、进一步开发马铃薯淀粉体细胞器生物反应器奠定了基础。
Abstract:
-

参考文献/References:

[1]李珺. 几种新型细胞器植物生物反应器的研究[J]. 生物技术通报,2008(3):30-32.
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[3]季勤,Vincken J P,Reamakers K,等. 利用GBSSⅠ和SBD作为“锚”将荧光素酶定位到淀粉粒中效果的比较[J]. 自然科学进展,2006,16(6):679-683.
[4]Hunt H V,Denyer K,Packman L C,et al. Molecular basis of the waxy endosperm starch phenotype in broomcorn millet (Panicum miliaceum L.)[J]. Mol Biol Evol,2010,27(7):1478-1494.
[5]Tenorio G,Orea A,Romero J M,et al. Oscillation of mRNA level and activity of granule-bound starch synthase I in Arabidopsis leaves during the day/night cycle[J]. Plant Molecular Biology,2003,51(6):949-958.
[6]宋东光,黄大庆,王光清,等. 不同长度马铃薯GBSS基因启动子的块茎专一性表达的初报[J]. 复旦学报:自然科学版,1998,37(4):559-563.

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备注/Memo

备注/Memo:
收稿日期:2013-01-17
基金项目:内蒙古科技大学创新基金(编号:2011NCL005)。
作者简介:李珺(1979—),女,宁夏石嘴山人,硕士,研究方向为植物基因工程。E-mail:star1979@126.com。
更新日期/Last Update: 2013-08-25